x*********n 发帖数: 43 | 1 如题,该蛋白N端有一个transit peptide,想找与它互作的蛋白。用哪种方法比较好呢
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n********0 发帖数: 11 | 2 AP-MS easy and simple and u can fisher all the candidate proteins. Dont use
Y2H. |
x*********n 发帖数: 43 | 3 Thanks for your reply
Should I extract the chloroplast first and then do the IP? or just IP from
the crude extract?
use
【在 n********0 的大作中提到】![](/moin_static193/solenoid/img/up.png) : AP-MS easy and simple and u can fisher all the candidate proteins. Dont use : Y2H.
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m*******u 发帖数: 173 | 4 You will get cleaner IP if using chloroplast. |
x*********n 发帖数: 43 | 5 Do you have any protocol of coIP from chloroplast? I could not find one to
use.
Thanks very much
【在 m*******u 的大作中提到】![](/moin_static193/solenoid/img/up.png) : You will get cleaner IP if using chloroplast.
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