x*********n
发帖数: 43 | 1 如题，该蛋白N端有一个transit peptide，想找与它互作的蛋白。用哪种方法比较好呢
？ |
n********0
发帖数: 11 | 2 AP-MS easy and simple and u can fisher all the candidate proteins. Dont use
Y2H. |
x*********n
发帖数: 43 | 3 Thanks for your reply
Should I extract the chloroplast first and then do the IP? or just IP from
the crude extract?
use
【在 n********0 的大作中提到】
: AP-MS easy and simple and u can fisher all the candidate proteins. Dont use
: Y2H.
|
m*******u
发帖数: 173 | 4 You will get cleaner IP if using chloroplast. |
x*********n
发帖数: 43 | 5 Do you have any protocol of coIP from chloroplast? I could not find one to
use.
Thanks very much
【在 m*******u 的大作中提到】
: You will get cleaner IP if using chloroplast.
|
