V******t
发帖数: 444 | 1 我想测试某个reagent，能引起细胞apoptosis的。是用IC50还是LD50为好，怎么计算？
比如，处理24小时。
浓度 细胞凋亡率
0 0.1％
2 5%
6 15%
18 34%
54 57%
怎么计算？
多谢！ | h*******o
发帖数: 4884 | 2 I would use EC50 in this case. | A******y
发帖数: 2041 | 3 The correct name is LD50 or ED50, but the mathematical equation is the same.
I hate to tell you that you don't have enough point to determine your LD50
. You need at least two log scale, but it look like your LD50 is around 50
(unit?). If it is microM, you are wasting your time. | j******n
发帖数: 941 | 4 不严格来说你用以上任何一个都说得过去 不过权衡一下我也最推荐用EC50
至于是用C（Concentration）还是D（Dose）这个也没严格规定
按我的习惯，体内毒理实验用D，体外细胞实验用C | V******t
发帖数: 444 | 5 没有，数据是我随便写了当例子的。
能否告知具体如何算？
浓度设计要用有log scal？比如呢，0nM， 10nM，然后呢？
太谢谢了。
same.
LD50
50
【在 A******y 的大作中提到】
: The correct name is LD50 or ED50, but the mathematical equation is the same.
: I hate to tell you that you don't have enough point to determine your LD50
: . You need at least two log scale, but it look like your LD50 is around 50
: (unit?). If it is microM, you are wasting your time.
| A******y
发帖数: 2041 | 6 Btw, I made a mistake, the name should be EC50 (effective concentration).
LD (lethal dose) is usually used in vivo for animal studies.
This is how my lab does EC50: We do 12 point titration either (1/2 or 1/3)
dilution per dilution. For example, for single digit microM compounds, we
do 200 micorM, 100 micorM, 50 microM, for 12 (Log[concentration] will be
your X-axis) points...After that we determine the viability of the cells (Y
axis). Then, you use a non-linear fit program (Prism is the most popular
one I think) to determine the EC50.
【在 V******t 的大作中提到】
: 没有，数据是我随便写了当例子的。
: 能否告知具体如何算？
: 浓度设计要用有log scal？比如呢，0nM， 10nM，然后呢？
: 太谢谢了。
:
: same.
: LD50
: 50
| V******t
发帖数: 444 | 7 谢谢！！
)
Y
【在 A******y 的大作中提到】
: Btw, I made a mistake, the name should be EC50 (effective concentration).
: LD (lethal dose) is usually used in vivo for animal studies.
: This is how my lab does EC50: We do 12 point titration either (1/2 or 1/3)
: dilution per dilution. For example, for single digit microM compounds, we
: do 200 micorM, 100 micorM, 50 microM, for 12 (Log[concentration] will be
: your X-axis) points...After that we determine the viability of the cells (Y
: axis). Then, you use a non-linear fit program (Prism is the most popular
: one I think) to determine the EC50.
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