b******3
发帖数: 62 | 1 I am using Varian 400MHz NMR to get a P31 signal, but I always get a flat
baseline. Is there anything I need to change for P31 NMR, like a different
probe? Thanks for any suggestions. | s*******h
发帖数: 3731 | 2 did u use the right sequence/method to run P NMR?
also check your probe to make sure it can be used for P31...
【在 b******3 的大作中提到】
: I am using Varian 400MHz NMR to get a P31 signal, but I always get a flat
: baseline. Is there anything I need to change for P31 NMR, like a different
: probe? Thanks for any suggestions.
| B*******y
发帖数: 766 | 3 看来还是我们有机千老的共同话题多阿，还有就是probe需要tune到P31的频率上
【在 s*******h 的大作中提到】
: did u use the right sequence/method to run P NMR?
: also check your probe to make sure it can be used for P31...
| b******3
发帖数: 62 | 4 Thanks for your suggestions. I wonder how to check whether the probe is
right for P31 and what specific sequence/method should be used. | u*********t
发帖数: 45 | 5 I am using Brukier. While the program is different from yours, the idea
should be the same.
In the acquisition program, you can set up what nuclear you want to look at.
after loading the program, you should hear a click at the probe head, this
switches the probe head from previous to P31. If the previous is also P21,
there is no click sound and if you want to futher check, you can, in Brukier
, type Wobb to confirm the frequence if it is of P31.
am
Usually, you can ask the NMR administrator for this.
【在 b******3 的大作中提到】
: Thanks for your suggestions. I wonder how to check whether the probe is
: right for P31 and what specific sequence/method should be used.
| c**t
发帖数: 244 | 6 听上去更像tuning/matching problem
【在 b******3 的大作中提到】
: I am using Varian 400MHz NMR to get a P31 signal, but I always get a flat
: baseline. Is there anything I need to change for P31 NMR, like a different
: probe? Thanks for any suggestions.
| c**t
发帖数: 244 | 7 Bruker
这种情况确实应该问administrator 尽量不要自己瞎鼓捣罢。
at.
this
Brukier
【在 u*********t 的大作中提到】
: I am using Brukier. While the program is different from yours, the idea
: should be the same.
: In the acquisition program, you can set up what nuclear you want to look at.
: after loading the program, you should hear a click at the probe head, this
: switches the probe head from previous to P31. If the previous is also P21,
: there is no click sound and if you want to futher check, you can, in Brukier
: , type Wobb to confirm the frequence if it is of P31.
: am
: Usually, you can ask the NMR administrator for this.
| b******3
发帖数: 62 | 8 Thank you guys for the comments. I just checked with Varian tech support and
confirmed that I have to tune the probe to the P31 frequency. But I cannot
do it automatically, I will have to tune it manually. There is no one else
in the company did that before, so I will have wait for Varian tech personel
to come. But it will take sveral month I guess. Thanks anyway.
【在 b******3 的大作中提到】
: I am using Varian 400MHz NMR to get a P31 signal, but I always get a flat
: baseline. Is there anything I need to change for P31 NMR, like a different
: probe? Thanks for any suggestions.
| m***c
发帖数: 1403 | 9 ask NMR groups/technicians in universities around your area, anyone can do
it, do not have
to wait a few months for a Varian engineer to fly over just for an one hour
job.
and
cannot
personel
【在 b******3 的大作中提到】
: Thank you guys for the comments. I just checked with Varian tech support and
: confirmed that I have to tune the probe to the P31 frequency. But I cannot
: do it automatically, I will have to tune it manually. There is no one else
: in the company did that before, so I will have wait for Varian tech personel
: to come. But it will take sveral month I guess. Thanks anyway.
| g*****n
发帖数: 36 | 10 Did you lock the signal successful?
And check you detecting nuclear, it should be 31P.
If all correct, then you should check tuning/matching.
Here is a a useful NMR website
http://bbs.chinanmr.cn | c**t
发帖数: 244 | 11 正解
其实很简单，不需要一小时那么长
do
hour
【在 m***c 的大作中提到】
: ask NMR groups/technicians in universities around your area, anyone can do
: it, do not have
: to wait a few months for a Varian engineer to fly over just for an one hour
: job.
:
: and
: cannot
: personel
| u*********t
发帖数: 45 | 12 Do not need go for Varian technician or engineer.
The frequency tune and match should be done manually with adjusting two
rodes in Bruker. One rode is labeled T, standing for Tune, and the other is
labeled M, meaning Match. I am not familiar with Varian, but feel it should
be the same as Bruker. It also depends on what probe head you have and you
have to find the right rodes for P31, in my machine, it is written as X.
I am around NYC and if it is close to you, I can take a look at your NMR and
see if I can work out for you.
【在 c**t 的大作中提到】
: 正解
: 其实很简单，不需要一小时那么长
:
: do
: hour
| u*********t
发帖数: 45 | 13 Do not need go for Varian technician or engineer.
The frequency tune and match should be done manually with adjusting two
rodes in Bruker. One rode is labeled T, standing for Tune, and the other is
labeled M, meaning Match. I am not familiar with Varian, but feel it should
be the same as Bruker. It also depends on what probe head you have and you
have to find the right rodes for P31, in my machine, it is written as X.
I am around NYC and if it is close to you, I can take a look at your NMR and
see if I can work out for you.
【在 c**t 的大作中提到】
: 正解
: 其实很简单，不需要一小时那么长
:
: do
: hour
| b******3
发帖数: 62 | 14 Thank you very much. But I am in LA area.
is
should
and
【在 u*********t 的大作中提到】
: Do not need go for Varian technician or engineer.
: The frequency tune and match should be done manually with adjusting two
: rodes in Bruker. One rode is labeled T, standing for Tune, and the other is
: labeled M, meaning Match. I am not familiar with Varian, but feel it should
: be the same as Bruker. It also depends on what probe head you have and you
: have to find the right rodes for P31, in my machine, it is written as X.
: I am around NYC and if it is close to you, I can take a look at your NMR and
: see if I can work out for you.
|
|
