c******n
发帖数: 16403 | 1 【 以下文字转载自 Military 讨论区 】
发信人: sandvik (注册商标), 信区: Military
标 题: 印度博后暗中破坏组员的实验半年终于被捕(图)
发信站: BBS 未名空间站 (Wed Nov 24 17:14:44 2010, 美东)
Vipul Bhrigu，Toledo大学的博士，到Univ of Michigan Comprehensive Cancer
Center做博后。从09年12月开始“细致而系统”的暗中破坏同组博士女生Heather Ames
的样品。
女博士渐渐发现了问题，开始还以为是自己犯了错误，采取了一些措施防止出错，
甚至拿到未婚夫的实验室去做，总是有各种各样的问题。怀疑有人破坏，说给朋友，导
师，学校相关部门听，都劝她是不是自己多虑了，甚至认为是她自己工作不顺利，试图
归结于其他原因。
直到出现media上被洒酒精，并且接二连三的发生，终于正式由警方介入。女博士
被审问两次，测谎一次，才给实验室安装了两个摄像头，并且非常震惊（对于他们，对
于我肯定是理所当然）的发现实验室新来的，“和蔼可亲，友好，健谈”的印度博后
Vip... 阅读全帖 |
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C****g
发帖数: 2220 | 2 泽 blotting
东 east
所以，应该仿造 生物wsn 的常用工具
命名为
Mao North Blotting
Mao South Blotting
Mao West Blotting
Mao East Blotting |
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G**********e
发帖数: 11693 | 3 第一 尽量少洗 穿的时候加倍注意 别穿着到处跑 出很多汗沾很多灰
第二 可以局部洗就不要全洗
第三 可以不用任何洗涤剂就不要用。club soda最好
局部洗：衣服放平脏的地方下面垫张毛巾 在脏的地方洒一点club soda，然后用paper
towel或者毛巾blot，动作要轻一点 一定只是blot，千万不能抹，会把那块料子撑大的
，重复若干次。尽量争取只用这个方式就把衣服弄干净了。一般滴在衣服上的油如果及
时，club soda就能blot干净了。 如果club soda不够，再升级用稀释的mild soap or
shampoo同样方式blot，清也是同样方式用清水blot。垫在下面那张毛巾如果太湿了就
换到干的部分。完后用吹风机 低档冷风吹一下就可以了。
全洗 一般在穿过多次后为了清洗整体灰尘和汗的话 冷水和mild soap or shampoo就可
以。完了不要拧，凉起来 等excess water滴干但还是湿的时候放进烘干机 用heatless
airfluff一档 哄到衣服干为止 衣服需要在烘干机里一直转动不能停止 所以烘干机一
停必须马上取出衣服 否则会出褶
这样哄出... 阅读全帖 |
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h******y
发帖数: 351 | 4 我的建议是，在和老板争论是否有deletion之前，利用其他的办法证实你的PCR所发现
的结果。PCR是很容易出错的，如果你能利用Southern证实homozygous的deletion确实
存在，相信你的老板是会接受你的结论的。
Constance L. Cepko实验室最近retract了G&D上的一篇文章，很好的说明了PCR的易错
性。有兴趣的可以看看。
http://genesdev.cshlp.org.mutex.gmu.edu/content/25/12/1344.long
“We are writing to clarify the interpretation of the results from our above
-mentioned paper. In this study, we used two methods to examine the
structure of RNA from the mouse Math5 (Atoh7) locus. Our initial
characterization was an analysis of the Math... 阅读全帖 |
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r****o
发帖数: 105 | 5
blot stripping buffer:
100mM beta-Mecaptoethanol
2% SDS
625mM Tris.Hcl pH6.8
To make 50 ml stripping buffer:
41.5ml ddH2O
5ml 20% SDS
3.125ml 1M Tris.HCl pH6.8
0.352ml beta-Mecaptoethanol
To use the buffer to strip the blot:
1. incubate the blot in the stripping buffer for
30 min at 70 celcius degree
2. Wash the blot with PBST five times
Note: this protocol's condition is very hars |
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i*******n
发帖数: 48 | 6 Buffer needed:
stripping buffer:
2% SDS
100 mM beta-mercaptoethanol
50 mM Tris, pH 6.8
Procedure:
1. Heat stripping buffer to 50c in water bath.
2. Incubate blot with stripping buffer at 60c for 30-45 min with gentle
shaking.
3. Rinse blot several times in TBS.
4. Blot is now ready for re-block and blot.
always work,and also I tried stripping several times
stripping |
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Z******5
发帖数: 435 | 7 很简单，自己配就行了。
stripping buffer:
2% SDS
100 mM beta-mercaptoethanol
50 mM Tris, pH 6.8
Procedure:
1. Heat stripping buffer to 50c in water bath.
2. Incubate blot with stripping buffer at 50c for 15-30 min with gentle
shaking.
3. Rinse blot several times in TBS.
4. Blot is now ready for re-block and blot.
这个就很好用。 |
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h******y
发帖数: 351 | 8 How To Make Your Own ECL
ECL can be an expensive reagent in a lab, and what with it being involved in
the final stage of a western blot, it’s something you don’t want to have
to worry about too much. During my PhD, I was struggling with my western
blots for ages – it seemed I was doing everything right, I just wasn’t
getting any signal in the dark room. At the time I was using a pre-made ECL,
and a friend that I shared lab space with suggested I try her home-made
brew. I was sceptical at first, ... 阅读全帖 |
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L**y
发帖数: 40 | 9 有意者请寄简历到l***[email protected]
Title: Research Associate- Cell Culture
Local candidates only. PhD’s NOT being considered.
About us
Biotechnology Company locates in North Dartmouth, 40 miles south of Boston,
just minutes from University of Massachusetts-Dartmouth.
Job Description
We have an immediate opening for a part-time and possible convert to full-
time Research Associate in the Cell Biology field.
The primary role of this position includes cell culture, prepare and stock
media, maintenance of mult... 阅读全帖 |
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b***1
发帖数: 7 | 10 We are looking for an experienced research associate to join a team working
on stem cell and other technology development.
The successful candidate will have a strong background in mammalian cell
culture (ES cell experience is desirable), as well as extensive experience
in standard molecular biology techniques, including manipulation of plasmid
(vector construction, subcloning, site-directed mutagenesis), transfection,
Western Blot Southern Blot, Northern Blot, Immunofluorescence. Experience
wit |
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s******n
发帖数: 110 | 11 我帮洛杉矶另一所大学的朋友招一个技术员和一个博士后，生物医学方向。有意者请直
接与他联系。此广告六个月内有效。
Posting Details
Classification Title: Lab Technician
Working Title: Research Assistant
Campus: Charles Drew University of Medicine and Science
Region: Los Angeles, California
Job Summary:
Our lab at Division of Cancer Research and Training uses a wide range of
molecular and biochemistry approaches to understand pathogenesis of
metabolic disorder-associated breast cancer and discover novel therapies
from small molecular agents. As a ful... 阅读全帖 |
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S****S
发帖数: 293 | 12 One postdoctoral scholar and one junior scientist position are available
immediately in the Division of Gastroenterology, Hepatology and Nutrition of
the Department of Medicine at the University of Minnesota, Twin Cities.
Required/Preferred Qualifications:
Ph.D. required, or M.D.
More than three years of post-graduate research experience in the fields of
liver cancer, metabolic diseases, microRNA, and RNA drug delivery.
At least one first-author publications in top-tier journals.
Skills in molec... 阅读全帖 |
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L**y
发帖数: 40 | 13 【 以下文字转载自 JobHunting 讨论区 】
发信人: Lexy (Lei), 信区: JobHunting
标 题: 麻省南部小生物公司需part time一名，local only！
发信站: BBS 未名空间站 (Thu May 19 15:30:50 2011, 美东)
有意者请寄简历到l***[email protected]
Title: Research Associate- Cell Culture
Local candidates only. PhD’s NOT being considered.
About us
Biotechnology Company locates in North Dartmouth, 40 miles south of Boston,
just minutes from University of Massachusetts-Dartmouth.
Job Description
We have an immediate opening for a part-time and possible convert to full-
time Re... 阅读全帖 |
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q*c
发帖数: 17993 | 14 REI的这篇文章挺好，针对滑雪Goggle的选择，我转贴过来：
http://www.rei.com/learn/expert-advice/goggles.html
Goggles for Skiing and Snowboard: How to Choose
/How%20to%20Choose%20Ski%20and%20Snowboard%20Goggles.jpg
Goggles offer you better eye protection than sunglasses do when you're
snowboarding or skiing for the day.
At first glance, it may not be apparent why goggles range so widely in price
—from $25 to over $200. Here's how to make sense of the technology and find
the right pair for your needs.
Why Wear Goggles?
Th... 阅读全帖 |
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m******h
发帖数: 38 | 15 做一个Co-IP实验，检测骨架蛋白plectin(>500KD)与一个GTPase（A）的相互作用。内
源plectin,高表达A(A-flag)或 dominant negative A (DN-A-flag)。用plectin单抗做
IP，anti-flag blot 显示A-flag可以被co-IP下来，同样的co-IP GFP-negative
control 背景比较干净，而co-IP DN-A-flag有微弱信号（约为A-flag 的1/10）。
用做IP同样的antibody blot plectin,input 部分一切正常，显示相同的内源plectin;
问题出在 straight IP部分，同样的抗体同时blot同一块胶上的IP部分，没有任何信号
show out。这一结果已至少重复3次（重复性很好）。
考虑到plectin分子量巨大，试过不同的gel concentration 和transfer time。但始终
不能解决这一问题。
其它可能情况：
1. plectin与A相互作用后translocation 到膜结构中,导致常规lysis buffer不能裂解... 阅读全帖 |
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D*****r
发帖数: 6791 | 16 http://www.franknelte.net/Nelte_HTML/LUCIFER.htm
In the English version of the Bible the name "Lucifer" appears only one time
, in Isaiah 14:12. This verse reads:
"How are you fallen from heaven, O LUCIFER, son of the morning..." (Isaiah
14:12)
Now the word "Lucifer" is not an English word, but a LATIN word. And so the
question is:
WHO GAVE THE WORLD THIS LATIN NAME "LUCIFER"? AND WHY DID THEY GIVE US THIS
LATIN NAME?
HOW WE CAME TO HAVE THIS NAME "LUCIFER"
In 382 A.D. Pope Damasus commissioned ... 阅读全帖 |
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c*********r
发帖数: 181 | 17 Detecting protein–protein interactions by far western blotting
Yuliang Wu1, Qiang Li1 & Xing-Zhen Chen
Nature Protocols 2, 3278 - 3284 (2007)
Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot
Randy A. Hall
Methods in Molecular Biology, 2004, Volume 261, II, 167-174, DOI: 10.1385/1-
59259-762-9:167
大家新年快乐！
谢谢！ |
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g********0
发帖数: 6201 | 18 Cancer Postdoc on Probation for Falsifying Data
06/05/2012 Jesse Jenkins
A former postdoc at Brigham and Women’s Hospital and Harvard Medical School
who confessed to splicing together Western blots has been put on probation
by the federal government.
A former postdoc at Brigham and Women’s Hospital (BWH) and Harvard Medical
School who deliberately fabricated data in federally funded lung cancer
research has been put on probation by the government.
The postdoc, Jian Ma, fabricated images in a man... 阅读全帖 |
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a**********u
发帖数: 28450 | 19 【 以下文字转载自 WaterWorld 讨论区 】
发信人: monolith (on the road), 信区: WaterWorld
标 题: [请转生物版（第一天注册，只能发水版)问题急求] IP 问题，各位大牛请进
关键字: 生物版 IP问题
发信站: BBS 未名空间站 (Wed Apr 24 10:56:49 2013, 美东)
做一个Co-IP实验，检测骨架蛋白plectin(>500KD)与一个GTPase（A）的相互作用。内
源plectin,高表达A(A-flag)或 dominant negative A (DN-A-flag)。用plectin单抗做
IP，anti-flag blot 显示A-flag可以被co-IP下来，同样的co-IP GFP-negative
control 背景比较干净，而co-IP DN-A-flag有微弱信号（约为A-flag 的1/10）。
用做IP同样的antibody blot plectin,input 部分一切正常，显示相同的内源plectin;
问题出在 straight IP部分，同样的抗体同时blot同一块胶上... 阅读全帖 |
|
t******n
发帖数: 2939 | 20 【 以下文字转载自 WaterWorld 讨论区 】
发信人: monolith (on the road), 信区: WaterWorld
标 题: [请转生物版（第一天注册，只能发水版)问题急求] IP 问题，各位大牛请进
关键字: 生物版 IP问题
发信站: BBS 未名空间站 (Wed Apr 24 10:56:49 2013, 美东)
做一个Co-IP实验，检测骨架蛋白plectin(>500KD)与一个GTPase（A）的相互作用。内
源plectin,高表达A(A-flag)或 dominant negative A (DN-A-flag)。用plectin单抗做
IP，anti-flag blot 显示A-flag可以被co-IP下来，同样的co-IP GFP-negative
control 背景比较干净，而co-IP DN-A-flag有微弱信号（约为A-flag 的1/10）。
用做IP同样的antibody blot plectin,input 部分一切正常，显示相同的内源plectin;
问题出在 straight IP部分，同样的抗体同时blot同一块胶上... 阅读全帖 |
|
l**********1
发帖数: 5204 | 21 BMJ
Gut is not trash journal
this journal 2013 one review
web link:
http://gut.bmj.com/content/62/8/1093.extract
its pp1094 left column top
cited here stuff sentence,
>Surprisingly, Yan et al did not provide
>Southern blot data demonstrating accumulation
of HBV DNA replication intermediates,
as this electrophoretic pattern is
a signature of productive virus replication.
Only one subfigure shows some evidence
of cccDNA formation, which
defines a successful infection by HBV;
however, unlike HBV DN... 阅读全帖 |
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l**********k
发帖数: 189 | 22 是这样的。我们不在乎别人跟我们打价格战，因为不想把自己的公司做成小作坊。至少
我们的服务速度目前在国内还是最快的。为了节省成本，很多公司已经不再做southern
blot了，因为国内大家都不愿意用同位素。用Dig-probe价格太贵，很多公司也做不出
来。但我们依然认为southern blot 结果是金标准。PCR筛选大部分情况是对的，但万
一有假阳性，或是有非特异性重组，那最后还是我们的问题。所以我们依然做southern
blot。
的确象你说的，国内客户更关心速度还有质量。所以尽管我们的要价稍高，但我们在中
科院动物所，发育所，神经所，生化所，北大，清华等这些国内顶尖的科院机构，已经
有了非常好的信誉。
关于QC，我们有QC组，另外，每一个结果都有几层把关。很难说做到完美，毕竟公司还
很年轻，每碰到一个问题，就尽快建立规程，尽快解决吧。
乱。 |
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l**********k
发帖数: 189 | 23 国内做公司时间一长，各种感受都会出来。
Southern blot只是一项，再比如做核型分析等等, 经常做老鼠的比较了解一些，知道
这些非常必要。现在国内很多都是以前做其他专业开始转到小鼠的。因为从来没有做过
小鼠，所以他们一般直接比价格，很难理解为什么要做这做那。
最近还有好多在别的地方做了一两年了，因为没有进展想把课题转到我们这里来。问打
靶载体怎们设计的，southern blot探针优化过没有。结果他们都没有收到过设计方案
，只听说现在打靶载体做完了，拿不到细胞，或是拿不到老鼠。申请的基金结不了题。
我们想帮吧，载体设计方案可能跟我们的不一样，或是根本没有设计用来做southern
blot的酶切位点。即使拿到载体，如果我们去分析，去测序的话，还不如我们自己做载
体快。但让客户花两次钱又心疼他们。 |
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M*******C
发帖数: 183 | 24 以前我们用ECF底物，strip用0.2N NaOH 就好了。
现在ECF 买不着了，改用ECL plus，Thermo Fisher/Pierce （32132）的，发现用NaOH
没法strip了，第一次blot的带仍然在那里。 看了ECLplus 的说明书，他们叫用
Thermo Scientific™Restore™ Western Blot Stripping Buffer (
Product No. 21059) 或者Restore Plus Western Blot Stripping Buffer (Product
No. 46430). ， 价格 500ml/$130, 太贵了，strip 一张膜得10ml 吧，500ml 50张膜。
不知道成分是什么，想自己配点，求各位指点！谢谢 |
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g*******f
发帖数: 427 | 25 我想用一个蛋白的抗体来免疫沉淀这个蛋白，目的是要从组织中富集这个蛋白用来做质
谱看它的在体内的修饰情况。
我用抗体沉淀蛋白后，加dynabeads protein G,洗脱后跑western blot 看蛋白有没有
被沉淀下来。western blot用做IP的抗体检测，发现在55KDa有非常强的带，但是我的
目的蛋白是40KDa, 这个带应该不是protein G，因为protein G 只有17 kDa。 我想请
问这个带会是什么呢？用什么抗体做western blot 来检测这个ip 后的产物比较好？
多谢了 |
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j*j
发帖数: 5564 | 26 3k, 抱歉抱歉，我也没仔细看，受了那个blot的误导，以为收据上都是署名朱佳良呢
既然这样，那个blot的牢骚就更过分了
多谢指出！ |
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D**S
发帖数: 24887 | 27 如果你就好那口儿，无所谓，接着喝。
如果你是因为信了所谓红酒对心血管有好处的说法才饮用的，恭喜你了，丑闻来了！
University Suspects Fraud by a Researcher Who Studied Red Wine
A charge of widespread scientific fraud, involving 26 articles published in
11 journals, was leveled by the University of Connecticut today against
Dipak K. Das, one of its researchers, whose work reported health benefits in
red wine.
Many of the articles reported positive effects from resveratrol, an
ingredient of red wine thought to promote longevity in laboratory animals.
The... 阅读全帖 |
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a***k
发帖数: 1038 | 28 【 以下文字转载自 paladin 讨论区 】
发信人: atack (小军号), 信区: paladin
标 题: 红死病 by 杰克.伦敦
发信站: BBS 未名空间站 (Sat Oct 11 07:40:15 2014, 美东)
I
The way led along upon what had once been the embankment of a railroad. But
no train had run upon it for many years. The forest on either side swelled
up the slopes of the embankment and crested across it in a green wave of
trees and bushes. The trail was as narrow as a man's body, and was no more
than a wild-animal runway.
Occasionally, a piece of rusty iron, showing through ... 阅读全帖 |
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C*I
发帖数: 4736 | 29 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
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C*I
发帖数: 4736 | 30 一直在误导，现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人，必须
经过一个中间宿体性的其它动物才能传染给人。所以，病毒发生后，就故意误导全国人
民去海鲜市场找证据，找其它野生动物的麻烦。 而且还是病毒所去找的，找完了还装
模做样化验呀，分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
，一直不敢说，说了其它相关已经在人就会去早那种动物监测。 所以压根不说，打马
虎眼。
事实上，早在2013 年，就是这个武汉病毒研究所，已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒，其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体，并能够感
染人的细胞。对此新发现，武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”，以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说，从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus， 可以不经过
其它受体/宿体，而直接传染给人。 他们... 阅读全帖 |
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C*I
发帖数: 4736 | 31 Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖 |
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M*****n
发帖数: 16729 | 32 帮朋友问的，
因为data太多，必须削减一些，那么哪些可以去掉呢？
（1）knockout mice已经做出来了，是不是那targeting strategy就不需要show? 有
southern blot和western blot就足够了？
（2）有很多quatinfication data,是不是可以省掉graph, 就用文字表述？
（3）有些试验在KO MEF和siRNA knockdown cell lines都做了，是不是可以只show一
些MEF data,提一下knockdown confirm同样结果？
（4）reviewer提出某个sub-aim 缺少prelim data,目前作了一些，是把这个sub-aim全
去掉好呢，还是把data放上，justify这个sub-aim是可行的？
（5）reviewer提出过于ambitious, 怎么address这个问题？是不是应该减掉一些sub-
aim?比如上面（4）这种情况？
(6) reviewer说推荐信写得太好，没有指出candidate的缺点weakness, 这个怎么弄？难道让referee非要说几个缺点出... 阅读全帖 |
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Q******o
发帖数: 718 | 33 PAINTED MANTELS: Often, much of the soot on painted mantels can be removed
by using a dry cleaning sponge, such as Mr. Clean Magic Eraser. Just rub in
small circles until soot disappears. For stubborn soot, mix a solution of
warm water and ammonia in a bucket---about 1 cup ammonia to gallon water is
the right proportion. Soak a clean rag in the solution, wring out excess,
and wipe mantel. Let dry, then repeat if necessary.
UNPAINTED BRICK: First, lay down a drop cloth, plastic sheeting or old
t... 阅读全帖 |
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b********a
发帖数: 79 | 34 我现在怀孕21周，在生化试验室工作。自己没有做过任何放射性试验，但试验室里有很
多人做。怀孕后我都倍加小心，但是今天竟然疏忽了！在一个oven边上前后累计操作试
验一小时左右，之后才发现oven里面有人正在做northern blot！而且中间还拿放
northern blot的玻璃试管两次。
发现以后真实后悔不迭。不知道这在oven前面放射性还大不，会对宝宝有影响不？？？ |
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C*****h
发帖数: 926 | 36 【 以下文字转载自 Biology 讨论区 】
发信人: Carwash (for+free), 信区: Biology
标 题: 3T3 transfection效率太低
发信站: BBS 未名空间站 (Wed Apr 28 21:47:39 2010, 美东)
用lipofectamine 2000转染3T3细胞，但是transfection效率太低（不到1％），
不知道是怎么回事？
0.8 ug plasmid DNA + 2 ul lipofectamine
转染1x10^5细胞。
不知道大家是怎么检测shRNA的knockdown的效果？
我是把带有shRNA的plasmid，转染到3T3细胞，然后用western blot检测蛋白质，
看目标蛋白是不是减少了。
可是现在转染效率不到1％，就算shRNA把那1％的细胞内的目标蛋白全部knockdown，
总个western blot还剩下99％的蛋白质。
有什么办法提高3T3细胞的转染率？有人转染过这个细胞系吗？请帮忙指教一下。谢谢。 |
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p********n
发帖数: 27 | 37 【 以下文字转载自 Boston 讨论区 】
发信人: pureoxygen (pureoxygen), 信区: Boston
标 题: 康缘药业招聘启事
关键字: 南京 康缘 招聘
发信站: BBS 未名空间站 (Wed Apr 1 23:15:35 2015, 美东)
康缘药业招聘启事（Career Opportunity）
1. 招聘单位（Company）：
江苏康缘药业股份有限公司（中国上市企业 600557）现代中药研究院
2. 聘任岗位（Position）：
高级研究员Senior Scientist
3. 工作地点（Work Location）：
江苏南京、江苏连云港 Nanjing, Jiangsu Province; Lianyungang, Jiangsu
Province
4. 招聘人数(Number of Recruitment)： 4
5. 研究方向（Direction of Research）：
中药及天然药物、化药、生物药的研发与分子机制研究
R&D in molecular mechanism of traditional Chinese medic... 阅读全帖 |
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G****e
发帖数: 11198 | 38 如何让你的牙齿雪白--HOW TO MAKE YOUR TEETH 'SNOW WHITE'-
Put a tiny bit of toothpaste into a small cup,mix in one teaspoon baking
soda plus one teaspoon of hydrogen peroxide, and half a teaspoon water.
Thoroughly mix then brush your teeth for two minutes. Remember to do it once
a week until you have reached the results you want. Once your teeth are
good and white, limit yourself to using the whitening treatment once every
month or two.
3% hydrogen peroxide CVS/Walgreen有卖，我前天去买，一瓶0.99，buy 2 get 1
free。于是买... 阅读全帖 |
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m*********g
发帖数: 10735 | 39 只有1%浓度
“纯”的意思就是没有其他东西在里面
你看看review就知道了，特别适合acne prone skin
Not Impressed at First, but Now I'm Hooked for Life, September 4, 2011
By Celeste D. "11:11" (Boulder, Colorado) - See all my reviewsAmazon
Verified Purchase(What's this?)
This review is from: Hyaluronic Acid Serum 100% Pure 2 oz. (Health and
Beauty)
I absolutely love this serum. I have to say though, it doesn't work very
well on its own....meaning, you have to use other products for this serum to
meet its full potential.
When I first bo... 阅读全帖 |
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i*****s
发帖数: 438 | 40 不懂生物，下面这一段的两个问题是怎么回事？
方舟子真的是滥竽充数吗？
因为他得到博士靠得就是这篇论文。nile去看了一下，这篇论文中，
图三BD和图四BC western blot（蛋白印迹）没有分子量标记。western
blot 为的是检测目标蛋白在细胞中的含量。通过用特异的与这种蛋白
结合的抗体杂交显示印迹而证实蛋白的存在。这个印迹必须出现在一个
正确的分子量标记的位置，否则要么抗体是错误的，要么显示出来的是
非特异条带。
更加严重的图四B和C居然是五次实验的结果拼接而成。如果印迹杂交
结果允许拼接，任何人间奇迹都可以创造出来。 |
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a***k
发帖数: 1038 | 41 I
The way led along upon what had once been the embankment of a railroad. But
no train had run upon it for many years. The forest on either side swelled
up the slopes of the embankment and crested across it in a green wave of
trees and bushes. The trail was as narrow as a man's body, and was no more
than a wild-animal runway.
Occasionally, a piece of rusty iron, showing through the forest-mold,
advertised that the rail and the ties still remained. In one place, a ten-
inch tree, bursting through... 阅读全帖 |
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l**********t
发帖数: 5754 | 42 chapter 8.2 Faith Is Again The Key
As for forgiveness, so equally for the coming upon us of the Holy Spirit,
the whole question is one of faith. As soon as we see the Lord Jesus on the
Cross, we know our sins are forgiven; and as soon as we see the Lord Jesus
on the Throne, we know the Holy Spirit has been poured out upon us. The
basis upon which we receive the enduement of the Holy Spirit is not our
praying and fasting and waiting, but the exaltation of Christ. Those who
emphasize tarrying and ... 阅读全帖 |
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l**********t
发帖数: 5754 | 43 chapter 8.2 Faith Is Again The Key
As for forgiveness, so equally for the coming upon us of the Holy Spirit,
the whole question is one of faith. As soon as we see the Lord Jesus on the
Cross, we know our sins are forgiven; and as soon as we see the Lord Jesus
on the Throne, we know the Holy Spirit has been poured out upon us. The
basis upon which we receive the enduement of the Holy Spirit is not our
praying and fasting and waiting, but the exaltation of Christ. Those who
emphasize tarrying and ... 阅读全帖 |
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s*****o
发帖数: 22187 | 44 【 以下文字转载自 Military 讨论区 】
发信人: DSJS (DSJS), 信区: Military
标 题: 喜欢喝红酒的小心了
发信站: BBS 未名空间站 (Thu Jan 12 16:07:02 2012, 美东)
如果你就好那口儿，无所谓，接着喝。
如果你是因为信了所谓红酒对心血管有好处的说法才饮用的，恭喜你了，丑闻来了！
University Suspects Fraud by a Researcher Who Studied Red Wine
A charge of widespread scientific fraud, involving 26 articles published in
11 journals, was leveled by the University of Connecticut today against
Dipak K. Das, one of its researchers, whose work reported health benefits in
red wine.
Many of the articles reporte... 阅读全帖 |
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h******n
发帖数: 493 | 45 This approach is good in theory but whether it will work or not
largely depends on your luck and how well all these experiments
are performed.
I guess you have the sequence of this protein and you have some
idea of which try residues may be phosphorylated.
First thing you want to do is to pufify your protein and then
probe your protein with antibody specific to pY on western blot.
If you see it lights up on the western blot, most likely you
have some pY residues in your protein.
Then you proceed |
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m**********3
发帖数: 706 | 46 "However, currently, when I used these cells (several
passages cell culture) for examining phospho-AKT, upstream signals or
downstream signals such as phospho-GSK,phospho-S6K and some others in
western blot, it is difficult to obtain the signal from western blot"
Do you mean you can not get any signal for all these antibodies? Or there is
no difference between your stable cell line and the parental cell line? |
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C****1
发帖数: 89 | 47 新手我最近苦于纠结一个关于IP实验的问题，特发此帖向大家求教。
我的目的蛋白A如果在肿瘤细胞（此蛋白已证实该细胞里正常水平不表达）里过度表达
会含有FLAG tag（通过adenovirus vector infection）。我用此蛋白来pull down 细胞
里的另外一个蛋白B。我用FLAG 抗体IP, Western blot 检测此两种蛋白A和B。结果在
正常对照的样品发现anti－Flag 抗体也能非特异性的结合蛋白B，即在blot也能检测到
B的条带。
请问如何消除抗体非特异性结合其他蛋白，还是我在那些方面需要改进？谢谢。 |
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s******r
发帖数: 2876 | 48 换一个Flag抗体。
新手我最近苦于纠结一个关于IP实验的问题，特发此帖向大家求教。
我的目的蛋白A如果在肿瘤细胞（此蛋白已证实该细胞里正常水平不表达）里过度表达
会含有FLAG tag（通过adnovirus vector infection）。我用此蛋白来pull down 细胞
里的另外一个蛋白B。我用FLAG 抗体IP, Western blot 检测此两种蛋白A和B。结果在
正常对照的样品发现anti－Flag 抗体也能非特异性的结合蛋白B，即在blot也能检测到
B的条带。
请问如何消除抗体非特异性结合其他蛋白，还是我在那些方面需要改进？谢谢。 |
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c*********r
发帖数: 1312 | 49 和大家讨论一些实验的问题
背景：老板要一个融合蛋白，本来在海胆（Sea Urchin）里表达的，分子量~85KDa，叫
Dishevelled，至少有磷酸化的修饰，在Wnt pathway里很重要一蛋白。想和一个tag连
在一起，其实最主要的是想得尽可能多的有正确修饰、折叠的天然蛋白用来做Far-
western blotting研究蛋白相互作用。可是我们都没有表达真核蛋白的经验，用E.
coli肯定不行。in vitro转录可能表达量不够，做一个Far-Western Blotting要1~50
μg的蛋白用量，而且指不定得做多少次来摸条件和重复呢。
要求：1.正确的修饰、折叠等等；2. 表达量大
问题：1. 用什么kit，细胞可以达到以上要求？有人推荐老板用昆虫细胞。
2. 自己做还是找公司做？我倾向于找公司或lab合作做但不知道有哪些公司可以做。大
家推荐一下吧。想合作也可以，嘿嘿。
多谢！ |
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