z****e
发帖数: 2024 | 1 class Base {
public:
virtual Base* clone() =0;
};
class Derived : public Base {
public:
virtual Derived* clone(){
return new Derived(*this);
}
};
int main() {
Base* b = new Derived;
Derived* d2 = b->clone();
return 0;
}
error: invalid conversion from ‘Base*’ to ‘Derived*’ |
|
c*********e
发帖数: 16335 | 2 什么是directive?
project的关键是，要能同时选n行，然后clone这n行的数据，显示在grid里，之后能编
辑一下这些新数据，然后存进数据库。
你说的那些plugin里面，哪些能这么做？
以前见过别人用jqgrid来clone一行数据。现在可能我再加几行code就能同时clone多行
数据了。
angular js做这个容易吗？如果容易上手，我就用angular js做了。
用angular js的同时，不能用jquery,ajax,对吗？ |
|
h****l
发帖数: 7290 | 3 If your two harddrive is not the same. You may damage the hard disk by ghost!
(Of course you can repair the harddisk later)
Be careful!
Three years ago I use ghost to clone from a 500Mb disk to a 1 Gb disk, It used
my 2 days to repair the later one :(
The corroct step is:
clone the old disk to a file, then clone the file to the new disk.
Am I wrong somewhere? |
|
M****e
发帖数: 70 | 4 with my experience, the suggestion is to check the PCR product
first. i would rather not digest the PCR product, actually, it
is better if you can subclone it first. for double digest with
NheI and XhoI, if you are using NEB buffer, use buffer2. digest
the insert and vector for enough time, and make sure your enzymes
are good (i think so from your description). unless the cloned
gene is harmful to the bacteria host, the cloning problem often
come from incomplete digest for such cloning strategy. |
|
M****e
发帖数: 70 | 5 this is a BIG problem. so what is the gene? and from what
kind of organism would u like to clone it? i have only a
couple of suggestions. first of all, check the RACE primers.
have you tried several different primers? 2nd, check the
known EST database and see whether there are severa image
clones available with which you can actually assemble the
clone. this gene might have very long 5'UTR. you may not
need the whole sequence for expression, though the UTR may
have regulatory sequences. 3rd, you |
|
c*******n
发帖数: 27 | 6 IMHP, for overlapping PCR, 10bp overlop is too short. Previous I tried
around 30bp overlap, it's ok, but sometimes takes a while. Then I always use
~50bp overlap, it always works with one or two tries. If your PCR not
working very good, try gradient PCR to find the best conditions.
You also can do it another way. After PCR, first try TA cloning, after
sequencing, cut it out and clone into your destiny plasmid. It always works
for me if having trouble cloning it directly into the destiny plasmid. |
|
n********k
发帖数: 2818 | 7 unless in special occasions/needed/desired(say u don't want the high
expression etc), what's the point making stable cell lines if you use
retroviral approach...I(many if not most) rarely keep them as stable lines..
..Nolan's lab is an excellent place for viral stuff...Yes, u can keep them
as stable cell lines,but you would have to take much precautions...single
clones is an old old(updated too) approach...of course u cannot do one
clones, u need at least dozens of clones intially and then narro |
|
s****l
发帖数: 395 | 8 pET系列载体的问题就是不能保证每个clone都能表达，一般一次挑5-6个clone然后看看
哪个能诱导吧，有些难表达的蛋白大概只有50%左右的clone可以诱导。 |
|
X***n
发帖数: 366 | 9 I cloned 400-500 bp miniarms cloned into ptdTomato-C1, and then linearized
it with EcoRI, so that the backbone was flanked by miniarms on both ends. I
electroporated the linearized plasmid into the induced Red recombinases in
SW102 carrying my BAC. All the clones turns to be empty. I succeed in the
other two using pKD46 system but failed in this one. And now I also fail
using SW102 system. What's the most possible reason? Thanks in advance. |
|
s******s
发帖数: 13035 | 10 你的细胞也不是clone, 测序也测得不是clone，还有啥jjww的。
先clone了你的片段再测序
吗？ |
|
n********k
发帖数: 2818 | 11 Joke of the day or what?
there is not a most shameless but more shameless!
Results: 33
1.
Viable fertile mice generated from fully pluripotent iPS cells derived from
adult somatic cells.
Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Wang X, Wang L, Zeng F,
Zhou Q.
Stem Cell Rev. 2010 Sep;6(3):390-7.PMID: 20549390 [PubMed - in process]
Related citations
2.
Production of mice using iPS cells and tetraploid complementation.
Zhao XY, Lv Z, Li W, Zeng F, Zhou Q.
Nat Protoc. 2010;5(5):963-71. Epu... 阅读全帖 |
|
n********k
发帖数: 2818 | 12 simply modify the vector first by fusion PCR (about anywhere 200bp-1kb)
introducing the desired cut site at
the desired position...and then do whatever you want...I would't trust any
PCR with a 13KB template without
sequencing...if u are luck, everything might be alright but u could end up
in dark having no clue what's gone
wrong...If you want to do the cloning in a single step which I won't
recommend if not an experienced one, just
go three piece or even four piece ligation... that way, u mig... 阅读全帖 |
|
v***a
发帖数: 1242 | 13 谢谢！看了不错，打算买了试试。
不知道你用过EMD（Calbiochem）的没？好多的clone啊，不知道怎么样？clone与clone
之间有什么区别吗？ |
|
m***f
发帖数: 1622 | 14
扯淡呢，看看高最近两年发表的文章吧
CELL STEM CELL, JBC, STEM CELL, PNAS，都是很不错的
就这样还不能PROMOTION? 在美国都可以tennure了
1. Wu T., Wang H., He J., Kang L., Jiang Y., Liu J., Zhang Y., Kou
Z., Liu L., Zhang X., Gao S. (2011) Reprogramming of trophoblast stem
cells into pluripotent stem cells by Oct4. Stem Cells 2011 Feb 8.
[Epub ahead of print].
2. Kang L., Wu T., Tao Y., Yuan Y., He J., Zhang Y., Luo T., Kou Z.,
Gao S. (2010) Viable mice produced from 3-factor induced pluripotent
stem (iPS) cells through tetrapl... 阅读全帖 |
|
b******s
发帖数: 1089 | 15 我做三片段连接。entry clone都测过序。但是三片段重组之后总是有一些问题。首先
，每次都有colony。我用不同片段和载体上的primers检测，有时候有些primer组合出
带，有些不出。有时候带还不错，但是size有些移动。最后只好又去测序。有些克隆的
峰乱糟糟叠在一起。有些有clean的峰，但是sequence又不完全match。克隆重复划板，
liquid culture都非常正常。
我一般程序是三片段entry clone加载体每个大约1-1.5ul，LR clonase 1ul，25度反应
18-24h, heat shock之后培养2-3h再涂板。反应和培养时间是否太长？重组反应时间太
长会有问题吗？
另外，我所有的东西都是从同实验室日本博后那要来的。entry clone我自己后来测过
序，但是载体没有。原本来怀疑是载体，就用酶切检测没成功。日本博后告诉我载体是
改造过的，真实序列和他给我的sequence可能不一样。我用几对primers检测，都是对
的。
有经验的能否指教一下可能问题在哪？谢谢 |
|
w*******i
发帖数: 119 | 16 两篇，谢谢
10. Nakayama K, Kelly SM, & Curtiss R, 3rd (1988) Construction of an Asd+
expression-cloning vector:
Stable maintenance and high level expression of cloned genes in a Salmonella
vaccine strain.
Bio/Technology 6:693-697.
11. Galan JE, Nakayama K, & Curtiss R, 3rd (1990) Cloning and
characterization of the asd gene of
Salmonella typhimurium: use in stable maintenance of recombinant plasmids in
Salmonella vaccine
strains. Gene 94(1):29-35.
请发d*****[email protected] 非常感谢 |
|
x********u
发帖数: 430 | 17 Position 1
The USDA-Agricultural Research Service, Soybean Genomics and Improvement
Laboratory in Beltsville, Maryland, is seeking a POSTDOCTORAL RESEARCH
ASSOCIATE for a TWO YEAR APPOINTMENT. Ph.D. is required. Salary is
commensurate with experience (GS-11+locality adjustment and benefits).
Citizenship restrictions apply http://www.afm.ars.usda.gov/hrd/jobs/visa/countries.htm. Veteran preferences apply. USDA/ARS is an equal opportunity provider and employer. The incumbent will use synth... 阅读全帖 |
|
s********r
发帖数: 312 | 18 佩服楼主的精神，但这篇文章让我读的悲从中来。WB应该算是生物实验中倒数第二简单
直白的了，倒数第一的是常规的subclone。
当时我在国内读硕士的时候也是一样，什么试剂都没有，自己配buffer，洗玻璃板，配
胶，那破玻璃板还漏，一上午有时候都配不好一块胶，用那种几百块钱的破抗体，买来
之后马上分装好，每个人分那么一丁点，自己的用完了要到处看人脸色借，自己配显影
液定影液，有时候要在暗室里等半个多小时才能看到条带。cloning也是一样，稍微稀
有点的酶都要去隔壁借个几微升回来，感受态细胞自己做，PCR也是连个kit都没有，提
质粒自己配buffer。
楼上有些人说的对，试剂钱是省不下来的。我整个硕士都在纠结这些trouble shooting
，各种做不出来跑不出来，老板指着鼻子说你们这些人分子生物学都是弱智。后来拿到
master赶紧跑路来美国读PhD,前几个月做这些都有心理阴影,洗膜曝光的时候都神经兮
兮的，生怕做不出来。结果WB和cloning怎么做怎么有，慢慢的才对自己恢复了信心。
现在看的那些细胞分子方面的paper，western都是一堆一堆的，cloning更是直接带... 阅读全帖 |
|
n********k
发帖数: 2818 | 19 a couple of questions:
1. Are you very experienced with molecular biology? no offense, u sounds a newbie to me...So a control question here: can you successful do other
short-ones say1-2kb or even less easily with the protocol...
2. do you know the exact sequence of the isoform(s), or you are trying to
use primers on the two extremes to fish out any possible isoforms(known or
unknow)?
3. With that, check your sequence complexity bioinformatic...anything
peculiar with your sequence?
4. if 1 and... 阅读全帖 |
|
n********k
发帖数: 2818 | 20 1. u sure u are using correct polymerase?
2. I don't recall I would purify my PCR product with PCR-cloning kit..I
might have, just don't remember now...but would be a control for you if you
concern about that step...
3. Ditch TA-cloning kit from invitrogen, expensive and not that good, esp
with >3kb...I use blunt PCR cloning kit from strategen, half price and
double-performance, no problem with up to 5kb or more...and work well with
most of HF polymerases...
,
题？ |
|
n********k
发帖数: 2818 | 21 Nothing much....from the one you asked about the reaction to adding 3'-A...
that's a very basic one for any experienced cloner...Anyway, trust me, ditch
TA-cloning kit if you still need to do lots of PCR-cloning...there are many
better ways to do cloning now... |
|
C*******e
发帖数: 4348 | 22 同不喜欢TA cloning
都用Blunt PCR cloning
Strategen的blunt PCR cloning kit跟invitrogen的pTOPO-BluntII相比如何？
invitrogen真是很贵很抠门
尤其他们家的感受态细胞
越来越稀有木有
you |
|
n********k
发帖数: 2818 | 23 First of all, I could be wrong but I am not sure you are on the right track
trouble-shooting so far...
As some said, this RNA extraction is not that good...That said, for a
cloning work, this may not be a problem at all...different goals require
different quality...As for protein or organic solvent etc, it may not be
that good but it is presumably fine, not horrible I would say..with today's
enzymes(very powerful), it is likely not a problem either...it would be very
bad if you were try to deter... 阅读全帖 |
|
c******r
发帖数: 3778 | 24 OK，所以目的是clone a gene，right？
那就分段clone啊，中间找几个unique的enzyme cutting site，分段clone了一拼，一
个月吧最多了。比你一次5kb容易多了
的。 |
|
n********k
发帖数: 2818 | 25 ditch TA cloning, go for blunt-end cloning, much better; or other
recombination-based cloning methods, even better. |
|
l**********1
发帖数: 5204 | 26 LZ pls try
XL-TOPO@ zero blunt kit
3kbp < insert size < 10 kbp
//www.invitrogen.com/1/1/17320-topo-xl-pcr-cloning-kit.html
and
//www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/
Cloning/PCR-cloning.html
original hint was from
http://www.mitbbs.com/article_t/Biology/31695611.html
3th floor |
|
r*******y
发帖数: 48 | 27 1. floxed 300 bp should not present any problem for recombination;
2. introducing a disgestion site is a good and reasonable design for future
southern verification, if there is no endogenous restriction site(s)
available for southern strategy;
3. you should pay more attention to the design of the arm length. Based on
my experience, over 5000bp/arm is recommended; shorter than this will reduce
the specific recombination efficiency, although some reports also suggested
that a shorter arm also wor... 阅读全帖 |
|
C********4
发帖数: 308 | 28 @新药研发观察
史上最苦B 的生物学家：Douglas Prasher 号称最先发现绿色荧光蛋白(GFP)，但是不
但没有得到诺贝尔奖，反而经历多次换实验室和下岗，最终离开科学领域，成了汽车司
机。最新进展：在61岁又回实验室做实验了。 http://t.cn/zYl8TMG 这是作为好消息来报导的，请勿做为反面宣传材料。
不知道钱永健给他什么title？ 61岁的老博后？？
What Ever Happened to Douglas Prasher?
The first researcher to clone the gene for green fluorescent protein, but
who was passed over for the 2008 Nobel Prize in Chemistry, is back in
academic science.
By Bob Grant | February 26, 2013
3 Comments
Print
1
Link this
Stumble
Tweet this
Prasher's profile picture fr... 阅读全帖 |
|
d****7
发帖数: 109 | 29 数据库里的序列不对？去看看IMGAGE clone，看看里面的plasmid，你的蛋白到底是S还
是T
我们组曾经研究过一个蛋白，我们买的IMAGE clone里的cds编码出来的蛋白有一个AA和
Entrenz/ensemble里的不一样，然后我们blast了一下，发现IMAGE clone的那个AA是
evolutionarily conserved，Entrez/ensemble里的并不保守，所以可能是数据库错了 |
|
s******c
发帖数: 331 | 30 您真有逻辑，您这这样是要比啥？比哪个专业更没落更没人学吗？我给你你一篇甲骨文
写的东西和一篇数学的论文，你会选哪一个？这样有可比性吗？真不理解还有这样比较
不同专业的，高中毕业的吗？我换个角度，学数学的有多少人，学生物的有多少人，在
这个基础上，学数学成功的比例有多大，学生物成功的比例有多大？难道不是应该人数
多竞争激烈成功比例小的才是更难学的要求更高的吗？
即便我follow你根本不知所谓的逻辑:
首先，你肯定选生物，是因为你没读过生物顶级论文，你只是拍脑袋想当然这个很容易。
其次，什么叫读懂？你看过生物的相关材料吗？你知道一篇top生物论文引用多少
references吗？你去看看那些annual review里的文章，你告诉我你可以用多长时间可
以把那些reference全部读一遍？我随便举个简单的例子，ribosome，给你一篇讲yeast
ribosome晶体结构的文章，你知道你需要读多少文献了解多少相关技术细节才能把它
搞清楚吗？你知道几十年的不同的biochemistry和biophysics的手段去研究得到的结论
，哪些可以被这个晶体结构解释以及哪些不能被这个晶体结构解... 阅读全帖 |
|
m*********D
发帖数: 1727 | 31 你的stable结果是单一clone还是一个selection pool？因单一clone涉及genome里的插
入位点，一般是作两个不相关的clone,或者一个pool。这个在早期的transgenic mouse
试验里，法国的一个实验室作的老鼠有特别的现象，回追出插入位点导致一个重要基因
的knockout.
transient的结果有时是不可靠。上面有人提到的表达量是一个问题。 |
|
l*******i
发帖数: 153 | 32 random integration确实是一个不容忽视的问题。别的细胞没做过，就人胚胎干细胞而
言，用CRISPR做knock-in, 基本上成功knock-in的clone中至少20%的clone含有一个以
上的random integration。所以，建议做knock-in时，尽量降低donor vector的用量，
所有knock-in的clone必须用southern blot筛一遍，确保没有random integration。 |
|
C****n
发帖数: 79 | 33 最近要将一个 3k+1k+2k的片段分别克隆到一个3k和8k 的载体上。试了两周Infusion
clone (Clontech, TAKARA）没弄出来。 Trouble-shooting过， 也用过overlap PCR
先assemble两个片段。但是仍然得到的是阴性结果（基于Colony-PCR和Restriction
enzyme digestion）。
之前一直用Infusion clone，虽然效率有高有低，但是这次彻底挂了。
理论上说Infusion比Gibson要效率高。当然其他方法如Golden Gate cloning， 还有
yeast recombination也许能行。不过似乎都有点麻烦。
请问各位大虾，对于我的这种情况，有没有其他类似高效快速的方法，或者克隆效率低
的可能性是什么呢？
不甚感谢。 |
|
j**u
发帖数: 1325 | 34 先装VMware，然后在里面装一个xp or 7，再make a clone of the xp or 7，最后安装
Mxroad。30天到期，delete the cloned xp or 7, re-cloning, re-install mxroad,
you get another 30 day。 |
|
m********k
发帖数: 69 | 35 Answer should be D.
Please notice this sentence: The nonresponsers have an increased frequeny of
one HLA type. This does not mean the lack of MHCII molecule expression ,
but means that this MHC II molecule can not bind and present the recombinant
polypeptide to Th cell and no immunologic reaction is induced. Meanwhile,
the responsers express other HLA subtypes instead in the same gene locus,
which can present the polypeptide to Th cell and then induce the production
of Ab.
As for negative sel... 阅读全帖 |
|
A*******s
发帖数: 9638 | 36 我们从小就学习马克思主义， 相信所有来自大陆的留学生都接受了正宗的马克思主义
教育， 万众一心就是这么达到的。 作为其中的一员， 小学到大学的政治课教育，让
我相信物质决定意识：先有人脑，才有意识。先有客观存在，才有对客观存在的认识。
到美国后， 开始接触宗教， 认识到很多人信神， 意识在先， 物质是上帝创造的。
我对两者不置可否。 说得通就行， 信不信由你。
两个星期前，我看了一个病人， 她对我说， 有一天早上， 她觉得她不是她自己， 而
是她妹妹， 一个上午都这样。 她说， 她很希望她是她妹妹， 可以一直保持下去。
我知道有些精神病人会这样， 但这个病人的说法让我多了个想法。 我怎么从来没有觉
得我是别人啊？ 好像这个自我意识是天生的， 所有人都take it for granted。
人的自我意识如果是物质决定的，这个物质每天都在变化， 昨天的我应该与今天的我
不同。 不错， 今天的我可能更成熟， 更知识化， 但我的自我意识是一样的， 我不
觉得昨天的那个我不是今天的这个我。 1%的不同都没有！
所以我提到了基因， 双胞胎和cloning。 大多数双胞胎长得像，80%相似应该不过... 阅读全帖 |
|
|
w*******y
发帖数: 60932 | 38 Star Wars Walkie Talkies $4.99 free shipping with Prime (FSSS) from Amazon
Set of two working walkie-talkies based on the Jedi Communicators
Communicate just like your favorite Jedi characters. These two walkie-talkie
-style communicators are modeled after the ones seen in The Clone Wars
animated series and theyre just as important to your missions as they are
in the show. Switch your communicator on and pivot the antenna to get in
touch with other Jedi warriors or issue commands to a clone tro... 阅读全帖 |
|
|
w*******y
发帖数: 60932 | 40 I just thought this is a great deal for an excellent HTPC keyboard.
I do not know if ebay buy it now auctions are allowed as deals.
I do not have any association with the ebay seller. I just bought one to
try out and bought another one recently because I liked the first one so
much.
I will give a mini review of this keyboard. I have owned a few HTPC
keyboards, the Lenovo with the trackball, the iPazzPort white one, the Rii
mini bluetooth, and this one the iPazzPort Rii.
LENOVO Trackball
I had ... 阅读全帖 |
|
g******0
发帖数: 1165 | 41 Wall Street Networking
Business - Investing
Description:
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Find over 100 links in this group to help you with jobs/internships,
Economics, Bloomberg tutorials, excel spreadsheets, fund manager interviews,
interview questions, learning about the industry (investment banking or
trading? You decide), developing contacts & learning how to make money in
the markets.
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Blackberry Addicts group
http:/... 阅读全帖 |
|
b*z
发帖数: 48 | 42 nature 416, 3 (2002), by erika check
[WASHINGTON] The United States and its European allies are once again on
a collision course over an international agreement. This time, the bone
of contention is a proposed global ban on human cloning, under
consideration at the United Nations (UN).
The UN committee began deliberating the proposal last week. But on the
first day of the talks, the American delegation said that any
anti-cloning agreement should also ban the 'therapeutic' cloning to form
human e |
|
c****e
发帖数: 279 | 43 【 以下文字转载自 Fujian 讨论区 】
发信人: clonee (CLONEE), 信区: Fujian
标 题: 厦门PX抗议事件居然由虎鲸套亲自强硬收场！
发信站: BBS 未名空间站 (Fri Feb 19 22:40:55 2010, 美东)
漳州杯具了，所有海鲜2年后不能吃了。。。。。。。
PX的老板是陈由豪
陈由豪打通了江泽民儿子江绵恒 的关系：
陳由豪，旗下翔鷺集團總裁俞新昌是台灣留美的高科技精英，當年在美國惠普任職時，
是江澤民之子江綿恆上司，是陳打通北京高層的主將。陳在中國布建政商關系，各級官
員都難避免他的銀彈“柔性斬首”。
俞新昌在台灣出生，祖籍浙江，在美國UIUC獲得計算机博士學位。一九八七年至九二年
幵始擔任中國惠普公司的總裁兼總經理。
江綿恆作為中國頂級“太子党”的先鋒人物，一向与台灣高層政經圈子的新一代緊密接
触，他曾与台灣“經營之神”王永慶之子王文洋合作，要打造IT業的新高峰，一度成為
媒体焦點。
陈由豪是台湾政府的「十大通緝要犯」第六，逃税十亿，台湾百姓对之恨之入骨
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福建古雷拟建台石化园区 年产值3000亿
2010年02月1 |
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d*****g
发帖数: 441 | 44 Just How Hot Is China Venture? Very!
Apr. 27 2011 - 6:22 pm
By REBECCA FANNIN
You don’t need a meter to gauge that after a two dry years, funds, deals
and IPOs are springing up in the busiest season yet. How long will it last?
Hard to tell, but I do hear echoes of the dotcom boom here.
Let’s count up the IPOs among the leading Chinese venture investment firms.
Qiming Ventures has five Chinese portfolio companies in the cue to go
public soon: ramped up car rental service eHi from Shanghai, dating... 阅读全帖 |
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c***s
发帖数: 70028 | 45 美国情色从业者“Clone-A-Willy”出价100万美金（约人民币635万元），要按照“小小贾”真实比例制作情趣玩具。
贾斯丁比伯独家被狗仔拍裸照
据台湾媒体报道，加拿大籍歌手贾斯汀·比伯（Justin Bieber）日前和女伴去小岛上别墅度假，全裸戏水的模样却被狗仔拍下来发上网，虽然经纪公司已经做紧急处理，警告大家不要再继续流传，但还是挡不住许多人的好奇心点开来看。现在就连情色从业者都看好这块大饼，要复制他下体1：1模型。
美国情色从业者“Clone-A-Willy”出价100万美金（约人民币635万元），要按照“小小贾”真实比例制作情趣玩具。据《heat》杂志报导，该公司写给小贾的信如下，“他是个知名的、有才华的人，而现在全世界都知道…他有个大X。”并表示这无损于对方的约会生活，反而还会增加唱片销量。
事实上，小贾斯汀一家对于下体流传在网络上的事不太在意，不仅爸爸杰瑞米（Jeremy Bieber）在个人推特上转发裸照并问：“你喂那个东西吃了什么？”他在受访时还大方聊“小小贾”的新称号，“我的粉丝有帮忙取名字，叫做Jerry。
新闻链接
比伯与卡戴珊的姐姐约会 对方大15岁有仨... 阅读全帖 |
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g*****1
发帖数: 666 | 46 常家蹲还是重复他的念咒一般的老生常谈，根本不值一提。
可读是读者的评论。
http://www.foreignpolicy.com/articles/2011/12/29/the_coming_col
MESS_MEDIA
9:18 PM ET
December 29, 2011
Yeah, I was wrong when I
Yeah, I was wrong when I predicted that the world would end on May 21, 2011.
It should end on October 21.
-Harold Camping
REPLY
JOESMITHII
2:44 AM ET
December 30, 2011
I guess, sooner or later,
I guess, sooner or later, Gordon will get used to admitting wrong
predictions. Fortunately, 2012 is around the corner, we don't need to wa... 阅读全帖 |
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n*****3
发帖数: 94 | 47 http://en.wikipedia.org/wiki/Fake_memoirs
(Fake memoirs form a category of literary forgery in which a wholly or
partially fabricated autobiography, memoir or journal of an individual is
presented as fact)
-------------------------------------------------------------------------
List of fake memoirs and journals
Matt McCarthy, Odd Man Out: A Year on the Mound with a Minor League Misfit
Viking (a division of Penguin Group USA) (February 2009) is a memoir
describing McCarthy's summer as a minor le... 阅读全帖 |
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e*****s
发帖数: 7359 | 48 观云图有感。
Every individual, no matter what species you are, is part of a larger game.
intra/interspecies competition, eating or being eaten, it's all about the
evolution of genetic information. You make it, you pass your copy of genetic
information to future generations which again will be playing the same game
, no guarantee for continuous winning. You lose; you still contribute to the
game by providing food, raw materials and lessens. The glory of any
advancement in this evolutionary process bear... 阅读全帖 |
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r*******g
发帖数: 32828 | 49 我的2011年版找工简历
王利民
2014年8月16日
（现注：当时，未列出2010年在Fox Chase Cancer Center的经历；没列出2005年在
Memorial Sloan-Kettering Cancer Center的经历；没列出2006年在陕西师范大学、
2009年在江南大学任教的经历。）
Career Summary:
PhD degree on Chemistry and post-doctoral research on protein
structure in the USA; Teaching (assistance) experience in the USA and
China; Customer service experience in the USA.
Work/ Research:
Jan 2006 - : various work in the USA, China, and home.
Sept. 2003- June 2005: New York State Department of Health
Post-doctorate
Re... 阅读全帖 |
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