S***l
发帖数: 323 | 1 definitely histone protein |
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a*m
发帖数: 1217 | 2 On April 2, 2010, Dr. Bing Zhu’s lab published a paper “Partition of
histone H3/H4 tetramers during DNA replication dependent chromatin assembly
” in science. |
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x********e
发帖数: 2275 | 3 Marcu MG, et al. Curcumin is an inhibitor of p300 histone acetylatransferase
. Med Chem 2006;2:169–174. [PubMed: 16787365]
我的信箱： c**********[email protected]
谢谢！ |
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J********3
发帖数: 3151 | 4 两篇Nature，谁也没敢提根治癌症！
Nature. 2010 Apr 8;464(7290):927-31. Epub 2010 Feb 17.
Proviral silencing in embryonic stem cells requires the histone
methyltransferase ESET.
Matsui T, Leung D, Miyashita H, Maksakova IA, Miyachi H, Kimura H, Tachibana
M, Lorincz MC, Shinkai Y.
Nature. 2010 Jan 14;463(7278):237-40.
KAP1 controls endogenous retroviruses in embryonic stem cells.
Rowe HM, Jakobsson J, Mesnard D, Rougemont J, Reynard S, Aktas T, Maillard
PV, Layard-Liesching H, Verp S, Marquis J, Spitz F, Consta |
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R****n
发帖数: 708 | 6 epigenetic有几个大分支，我们做的是DNA methylation detection.其他的还有
histone acetylation/methylation，别的我也不知道。DNA methylation和microarray
/next generation sequencing 结合比较紧密.可以做为癌症早期检测的评估手段。我
现在对methylation调控机理挺感兴趣，但是我们实验室不玩cell culture.大牛来讲讲
吧 |
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c******n
发帖数: 492 | 7 我在用HDAC inhibitor处理细胞。请问如何在排除了h1和h2的情况下，看看是不是通过
h3呢？
新手。
谢谢。 |
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a***e
发帖数: 1010 | 9 can you first test whether h3 modification are altered after HDAC inhibitor
treatment by western blot, MS-spec, or ChIP? |
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O******e
发帖数: 4845 | 10 老大今天心情不错哈。这么热心回答问题。要不要发点包子表示下？
inhibitor |
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c******n
发帖数: 492 | 11 thanks for answering. I think it is a good tip. |
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C******s
发帖数: 1282 | 12 帮忙找2篇文章吧。烂学校的图书馆找不到啊。多谢多谢。请发到 w***[email protected]
1, The HTLV-1 Tax protein: Revealing mechanisms of transcriptional
activation through histone acetylation and nucleosome disassembly
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
Volume 1799, Issues 3-4, March-April 2010, Pages 266-274
2, Chapter 7 Nuclear Receptor Repression: Regulatory Mechanisms and
Physiological Implications
Progress in Molecular Biology and Translational Science
Volume 87, 2009, Pages 235-259 |
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n********k
发帖数: 2818 | 13 Altered histone acetylation is associated with age-dependent memory
impairment in mice.
Peleg S, Sananbenesi F, Zovoilis A, Burkhardt S, Bahari-Javan S, Agis-Balboa
RC, Cota P, Wittnam JL, Gogol-
Doering A, Opitz L, Salinas-Riester G, Dettenhofer M, Kang H, Farinelli L,
Chen W, Fischer A.
Science. 2010 May 7;328(5979):753-6. Erratum in: Science. 2010 Jun 25;328(
5986):1634.
Recovery of learning and memory is associated with chromatin remodelling.
Fischer A, Sananbenesi F, Wang X, Dobbin M, Tsai |
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f*********6
发帖数: 94 | 14 rat anti phospho-Histone H3 (1:500, Upstate Biotechnology) |
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a****d
发帖数: 1919 | 15 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706
cell signaling tech |
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b*****e
发帖数: 288 | 16 随便去pubmed搜了下：
A large intergenic noncoding RNA induced by p53 mediates global gene
repression in the p53 response.
Huarte M, Guttman M, Feldser D, Garber M, Koziol MJ, Kenzelmann-Broz D,
Khalil AM, Zuk O, Amit I, Rabani M, Attardi LD, Regev A, Lander ES, Jacks T,
Rinn JL.
Cell. 2010 Aug 6;142(3):409-19.
Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer
metastasis.
Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T,
Argani P, Rinn JL, Wang Y, Brzoska P, ... 阅读全帖 |
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a***e
发帖数: 1010 | 17 In S. Pombe and A. Thaliana, miRNAs are able to induce histone modification
and heterochromatin formation, which eventually inhibit transcription.
However, it is still controversial whether miRNAs translocate into the
nucleus and inhibit trasncription in metazoans.
It is big leap if you can demonstrate this. |
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s******s
发帖数: 13035 | 18 btw, histone的modification到底能不能直接遗传？哪些能？
我看了一堆paper，model倒是一大堆，但是好像看不到直接证据，
很多也可以解释成secondary的effect |
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I*****y
发帖数: 6402 | 20 make yourself clear first
binding |
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R******d
发帖数: 1436 | 23 如果有人的T细胞（CD4+算T细胞吧）以外其他细胞类型的sytem level histone
modification
data，也欢迎提供文章和数据下载地址。
包子伺候。多谢了。 |
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R******d
发帖数: 1436 | 24 再问一句吧：
Keji Zhao CD4+ T cell的数据是不是没有提交到这个数据库？
他做了那么多种histone modification，这里都没有记录。 |
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h***l
发帖数: 168 | 25 some examples:
http://www.ncbi.nlm.nih.gov/gds
GSE24165
Title Histone H3K27ac separates active from poised enhancers and
predicts developmental state
Organism Mus musculus
Lab Jaenisch
GSE24164
Title ChIP-Seq of chromatin marks at distal enhancers in Mouse Embryonic
Stem Cells and adult tissues.
Organism Mus musculus
Lab Jaenisch
GSE15519
Title Expression and ChIP-seq analyses of embryonic stem cells,
extraembryonic endoderm stem cells, and trophoblast stem cells
Orga... 阅读全帖 |
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s****z
发帖数: 50 | 28 Is histone mRNA equally distributed in nuclei and cytoplasm? |
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c**a
发帖数: 94 | 29 就是Histone acetylation/deacetylation,蛋白放一起做in vitro assay. 有没有什么
trick啊？买来的recombinant protein都不管用，positive control都做不出来。不知
道到底
哪里出了问题。 |
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c**a
发帖数: 94 | 30 遇到高手了,讨教一下. 我用的是HDAC6, 从 Enzo 买的, 拿来做tubulin deAc, 这个在
2002的paper
中就show过了, 但是我做死都做不出来. 到底是买的protein失活了,还是反应需要特殊
的条件? 也
试过用Hela nuclear extract 加purified Histone, 也做不出来. 请高手明示. 另外,
你说的 TFA
substrate是什么?
most
though. |
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m*p
发帖数: 226 | 31 那是当然。 Histone 2B has to be expressed in nucleus and binds to DNA.
Thanks though! |
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m***f
发帖数: 1622 | 32
扯淡呢，看看高最近两年发表的文章吧
CELL STEM CELL, JBC, STEM CELL, PNAS，都是很不错的
就这样还不能PROMOTION? 在美国都可以tennure了
1. Wu T., Wang H., He J., Kang L., Jiang Y., Liu J., Zhang Y., Kou
Z., Liu L., Zhang X., Gao S. (2011) Reprogramming of trophoblast stem
cells into pluripotent stem cells by Oct4. Stem Cells 2011 Feb 8.
[Epub ahead of print].
2. Kang L., Wu T., Tao Y., Yuan Y., He J., Zhang Y., Luo T., Kou Z.,
Gao S. (2010) Viable mice produced from 3-factor induced pluripotent
stem (iPS) cells through tetrapl... 阅读全帖 |
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m*****i
发帖数: 29 | 33 Keji Zhao's top 3 hottest paper in 2010 seems unknown to all of you guys.
High-resolution profiling of histone methylations in the human genome
[PDF] from bmskorea.co.kr
FindIt@Towson…, DE Schones, Z Wang, G Wei, I Chepelev, K Zhao - Cell, 2007
- Elsevier
Cited by 1271 |
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w***m
发帖数: 10498 | 34 when you are talking about Epi, what do you really refer to, DNA
modification or histone modification or something else? |
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s******y
发帖数: 220 | 35 做subcellular fractionation 提取nucleus， 我的target 蛋白应该在cytosol和
nuclear fraction 都有。
可是用kit，只看到核有，用自己找的一个protocol只看到cytosol 有，也不知道核蛋
白提出来没。
请问nuclear loading control用什么好呢？ 我借了个lamin a的抗体，居然只在
cytosol里面有，不合理啊。
作为nuclear loading control， anti－histone是不好点？ 谢谢了。 |
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s******y
发帖数: 28562 | 36 我对这个也一直很有疑问。
histone protein 我看见有的文章用过，但是我自己试验的时候就很不好使，
即使是同一批细胞分成两部分来做都不consistent. 据说是因为那个蛋白很容易
沉淀。 |
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s********r
发帖数: 312 | 37 Please send to g******[email protected]
Cell Res. 2011 Mar 22. [Epub ahead of print]
Readers of histone modifications.
Yun M, Wu J, Workman JL, Li B.
Department of Molecular Biology, UT Southwestern Medical Center, Dallas, TX
75390-9148, USA;
Thanks a lot~~ |
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Z******5
发帖数: 435 | 38 The mammalian epigenome.
Bernstein BE, Meissner A, Lander ES.
Cell. 2007 Feb 23;128(4):669-81. Review.
PMID:17320505[PubMed - indexed for MEDLINE] |
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s*********t
发帖数: 600 | 39 太多了。
比如
做植物的Steve Jacobsen, Jian-Kang Zhu
做histone variants的 Steve Henikoff, Genevieve Almouzni, Carl Wu
做干细胞的Rudolf Jaenisch
做omics的Keji Zhao, Richard Young
做polycomb的Cavalli, Pirrotta
做DNA甲基化的Peter Jones, Adrian Bird, Wolf Reik, Azim Surani
做组蛋白修饰的Jerry Workman, Thomas Jenuwein, Tony Kouzarides，Shelley Berger，Ali Shilatifard
此外还有Rosenfeld， Shiv Grewal，Anjana Rao， Robert Kingston, 瑞士的Susan Gasser 。。。
你google Gurdon Conferences， Keystone, Cold spring harbor conference的epigenetics部分，给talk的都是大牛
哎，... 阅读全帖 |
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b****n
发帖数: 311 | 40 写的很不错。几天前看到这里讨论还想有空写个Anjana Rao的介绍，幸好没有献丑。
song的文章和Rao的工作比起来说跟风并不算过分。Rao的工作才是真正给力的原创！
近十年来，Nature抢到了RNAi， Cell抢到了ips, science好像落后了，即使这次拿了
DNA demethylase, 可惜histone demethylase早被cell抢了。 |
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s**s
发帖数: 122 | 41 Anjana Rao文章的第一作者是从yujiang Shi 实验室出来的。Yujiang Shi是Shi Yang
关于histone demethylase文章的第一作者。这个工作有可能原始的思想是从Yujiang
Shi实验室出来的。
板上有没有人知道些内幕？ |
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z*t
发帖数: 863 | 42 i don't know the connection personally, but Rao's paper is not using histone
demethylase catalytic domain to blast Tet out instead they used JBP1/2,
also Yi Zhang's review assgin their eurecha to the comtemplating over
thymidine salvage pathway, which is not what they mention in their original
paper.
Yang |
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n*********m
发帖数: 38 | 43 Look his publication
Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights
from structural and biochemical studies on human RPE.
Liang W, Ouyang S, Shaw N, Joachimiak A, Zhang R, Liu ZJ.
FASEB J. 2011 Feb;25(2):497-504. Epub 2010 Oct 5.
PMID:
20923965
[PubMed - indexed for MEDLINE]
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Wu D, Li Y, Song G, Cheng C, Zhang R, Joac... 阅读全帖 |
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z*t
发帖数: 863 | 44 他现在怎么不追epigenetics?
要我说现在structral biology其实对epigenetics更有指导意义哈，比如emory的
Xiaodong Cheng，从结构里推测DNMT3L可能得结合H3K9me3，并形成DNMT scaffold，相
当有指导性，还有不能不提的Dinsaw Partel对RNAi相关蛋白的结构和一系列Histone相
关的结构，对目前epigenetics很有帮助哈 |
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s*****t
发帖数: 107 | 45 正在做一个很有趣的基因的上游控制元件的ChIP assay。 发现这个基因ATG上游从14kb
的地方有一段3-4kb的地方没有Histon H3的binding。请问各位大虾该怎么解释？ |
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g****e
发帖数: 137 | 47
很复杂
很多, gene expression, DNA damage repair and many others
有 |
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d****7
发帖数: 109 | 48 这些问题都太笼统了，不好回答啊
问题一：很多，比如各种酶
问题二：也很多，比如基因的转录水平，甚至转录后的剪切都会影响
问题三：这我就不知道了 |
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s***m
发帖数: 6197 | 49 Pre- and Postimplantation Development of Swine-Cloned Embryos Derived from
Fibroblasts and Bone Marrow Cells after Inhibition of Histone Deacetylases
刊物：Cellular Reprogramming (Formerly "Cloning and Stem Cells") - 12(1):85-
94;
Please send to x****[email protected]
Thanks! |
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f*********e
发帖数: 24 | 50 Relation between MeCP2 and Rett syndrome
1999-10 Nat Gen Huda Y. Zoghbi Rett syndrome is caused by mutations
in X-linked MECP2, encoding methyl-CpG-binding protein 2
Mouse models: whole-body knockout, brain-specific, brain-region-specific,
knock-in,..., over-expression, rescue, ...
2001-03 Nat Gen Adrian Bird A mouse Mecp2-null mutation causes
neurological symptoms that mimic Rett syndrome
2001-03 Nat Gen Rudolf Jaenisch Deficiency of methyl-CpG bindi... 阅读全帖 |
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