r**********1
发帖数: 221 | 1 https://www.zerohedge.com/health/man-behind-global-coronavirus-pandemic
His bio (source):
Peng Zhou, Ph.D., researcher, team leader of bat virus infection and
immunity. He successively obtained bachelor's and doctoral degrees from
Henan University (2004) and Wuhan Institute of Virology, Chinese Academy of
Sciences (2010). During his doctorate, he was sent to the Australian Animal
Health Laboratory for study. He then carried out research work at Duke-Nus
Medical College in Australia and Singapore... 阅读全帖 |
|
h*********r
发帖数: 10182 | 2 黄茶代子现在傻眼了。白主子都变卦了。
Sean Hannity said Thursday he has “evolved” on immigration and now
supports a “pathway to citizenship.”
Hannity told his radio listeners Thursday afternoon that the United States
needs to “get rid of the immigration issue altogether.”
“It’s simple to me to fix it,” Hannity said. “I think you control the
border first. You create a pathway for those people that are here — you don
’t say you’ve got to go home. And that is a position that I’ve evolved on
. Because, you know what, ... 阅读全帖 |
|
l****z
发帖数: 29846 | 3 Report: Price tag for bill that provides amnesty is $6.3 trillion
May 6, 2013 | 10:42 am | Modified: May 6, 2013 at 10:50 am
Granting a pathway to citizenship for the nation’s 11 million illegal
immigrants would cost the country $6.3 trillion, according to a report
issued Monday by the conservative Heritage Foundation.
Authors Robert Rector and Jason Richwine argue that the massive influx of
newly legalized immigrants who are given so-called amnesty will strain the
system by creating millions mo... 阅读全帖 |
|
l****z
发帖数: 29846 | 4 McClintock: If Senate Amnesty Bill Passes, ‘We’ll Never Secure the Border’
July 25, 2013 - 11:54 AM
By Alissa Tabirian
Rep. Tom McClintock
Rep. Tom McClintock (R-Calif.)
(CNSNews.com) – “The Senate pathway [to citizenship] . . . is going to
make it very difficult to secure the border,” Rep. Tom McClintock (R-Calif.
) said during a “Conversations with Conservatives” panel discussion
Wednesday at the U.S. Capitol.
Insisting that border security remain a separate issue from immigration
reform, McCl... 阅读全帖 |
|
c*******4
发帖数: 1874 | 5 看得懂什么叫pathway to citizenship吗？
可以交豁免之后排队入籍也叫pathway，墨西哥130万人在排队，至少得排20年
：President Donald Trump says he’s open to a pathway to citizenship for
：hundreds of thousands of young people who were brought to the country as |
|
m****s
发帖数: 18160 | 6 【 以下文字转载自 Biology 讨论区 】
发信人: Suave911 (911), 信区: Biology
标 题: Post-doc Position Available in Cancer/Signal Transduction
发信站: BBS 未名空间站 (Fri Jun 21 10:21:47 2013, 美东)
Post-doc Position Available in Cancer/Signal Transduction
Molecular Cell Biology, Leiden University Medical Center, The Netherlands
A Post-Doctoral position is available in a dynamic, international and
interdisciplinary research team to study the molecular basis of subverted
TGF-β signal transduction in cancer.
Focus of the res... 阅读全帖 |
|
m****s
发帖数: 18160 | 7 【 以下文字转载自 Biology 讨论区 】
发信人: Suave911 (911), 信区: Biology
标 题: Post-doc Position in Vascular Biology/Signal Transduction
发信站: BBS 未名空间站 (Fri Jun 21 10:22:51 2013, 美东)
Post-doc Position in Vascular Biology/Signal Transduction
Molecular Cell Biology, Leiden University Medical Center, The Netherlands
A Post-Doctoral position is available in a dynamic, international and
interdisciplinary research team to study the molecular basis of subverted
TGF- signal transduction in cardiovascular biolog... 阅读全帖 |
|
a*******s
发帖数: 117 | 8 Yan, X.; Li, F.; Dozmorov, I.; Frank, M. B.; Dao, M.; Centola, M.; Cao, W.;
Hu, D., External Qi of Yan Xin Qigong induces cell death and gene expression
alterations promoting apoptosis and inhibiting proliferation, migration and
glucose metabolism in small-cell lung cancer cells. Molecular and Cellular
Biochemistry 2012, 363 (1-2), 245-255.(IF 2.329)
Yan, X.; Shen, H.; Jiang, H. J.; Hu, D.; Wang, J.; Wu, X. Q., External Qi of
Yan Xin Qigong Inhibits Activation of Akt, Erk1/2 and NF-kappa B and
I... 阅读全帖 |
|
发帖数: 1 | 9
Download pdf published by SfN (Chapter from 'The History of Neuroscience in
Autobiography, Volume 8' edited by Larry R. Squire)
Yuh-Nung Jan's CV
Lily Jan's CV
Yuh-Nung Jan and Lily Jan
Birth
Family History and Growing Up
National Taiwan University
The Hiking Trip to Shitou in the Spring of 1967
Graduate School Application
Graduate Study at Caltech (1968锟974)
Seymour Benzer Lab (1974锟977)
Steve Kuffler锟絪 Lab at H... 阅读全帖 |
|
m****s
发帖数: 18160 | 10 【 以下文字转载自 Biology 讨论区 】
发信人: Suave911 (911), 信区: Biology
标 题: Post-doc Position Available in Cancer/Signal Transduction
发信站: BBS 未名空间站 (Fri Jun 21 10:21:47 2013, 美东)
Post-doc Position Available in Cancer/Signal Transduction
Molecular Cell Biology, Leiden University Medical Center, The Netherlands
A Post-Doctoral position is available in a dynamic, international and
interdisciplinary research team to study the molecular basis of subverted
TGF-β signal transduction in cancer.
Focus of the res... 阅读全帖 |
|
m****s
发帖数: 18160 | 11 【 以下文字转载自 Biology 讨论区 】
发信人: Suave911 (911), 信区: Biology
标 题: Post-doc Position in Vascular Biology/Signal Transduction
发信站: BBS 未名空间站 (Fri Jun 21 10:22:51 2013, 美东)
Post-doc Position in Vascular Biology/Signal Transduction
Molecular Cell Biology, Leiden University Medical Center, The Netherlands
A Post-Doctoral position is available in a dynamic, international and
interdisciplinary research team to study the molecular basis of subverted
TGF- signal transduction in cardiovascular biolog... 阅读全帖 |
|
|
|
k**n
发帖数: 6198 | 14 其实中医的原理和西医是不矛盾的，和现在西医研究提倡的系统生物学不谋而合。
中药熬出来的就是一复合剂，是各种小分子，natural product的混合溶液。而西药一
般是一个靶向药物，高浓度，调控的是主要的几个基因pathway，就所谓头疼医头，脚
痛医脚。治疗癌症的药，oncogene被低调控了，但是可能其他的重要人体pathway也受
到影响，所以就有副作用
相对西药来说，中药混合液每种分子的浓度很低，所以副作用小，如果其中某一个分子
出来，高浓度给人吃，就可能是毒药，前面炒得很热的紫杉酮就是一个例子。最近中国
姚亿搞出来的那个中草药里面提出来的什么素，治疗痢疾最为有效，就是又一个
natural product可以作为西药典型的例子。而现在国内很多的这样那样的中药制剂，
其实有效成分是西药。比如一般的感冒清热冲剂，就是加了Acetaminophen,这个是
tylone的主要成分，所以就减轻感冒症状的效果。
就我个人来说，我比较倾向于中药制剂里面是有主导分子，然后很多别的小分子在细调
很多其他的基因pathway，这就是现在西医里面讲的gene interaction netwo... 阅读全帖 |
|
s***n
发帖数: 2366 | 15 http://www.npr.org/2012/06/20/155431944/asian-americans-on-the-
On Tuesday, the Pew Research Center released a study that found Asian-
Americans have surpassed Hispanics as the fastest growing racial group in
the U.S. Plus, the report showed that they're the highest earning and best-
educated. Host Michel Martin discusses the findings and the implications of
these demographic changes with three experts.
Copyright © 2012 National Public Radio®. For personal,
noncommercial use only. See ... 阅读全帖 |
|
t*******r
发帖数: 22634 | 16 我觉得 pathway A 首先是保证娃能坐下来做数学，否则理想再丰满、现实也是
骨感。
pathway B 不知道，但 pathway B 娃，很多是既懒惰又不切实际的骄傲，在
这个叛逆的 pre-teen 年龄也不好整。
有时候我觉得，学校数学老师的 function，也不仅仅是教数学。另一个重要
的 function 是确保娃娃们在车上、车车们在轨道上。这世界上靠谱的神娃
是极少数（反正我是一只神猪也没亲眼见过），大多数娃娃们教育面对的第
一个问题，并不是 academic excellence，而是咋对付这么不靠谱的娃娃们。 |
|
a*****g
发帖数: 19398 | 17 More school districts use no-cost online resources to supplement classroom
lessons and textbooks
When Tullahoma City Schools administrators started shopping for new social
studies textbooks in 2013, they were disappointed to find only a few options
aligned to the new Tennessee state standards. Rather than wait for newer
textbooks to be released, the district embarked on the ambitious project of
creating its own.
Two years later, the result is a series of social studies and math textbooks
that st... 阅读全帖 |
|
|
p********y
发帖数: 557 | 19 One Postdoctoral fellow in Epigenetic Modulation of Pain Signaling (
available immediately)
Senior faculty member in the Department of Medicine/Gastroenterology is
seeking a highly motivated post-doctoral fellow who is interested in
investigating the role of epigenetic pathways in regulating pain pathways.
Work will involve animal models and cultured cells, both neurons and
epithelial cells. Research efforts include performing small animal surgery,
developing molecular probes, and conducting im... 阅读全帖 |
|
z*h
发帖数: 773 | 20 Protein engineering and Innovative Biomanufacturing
Our mission is to integrate engineering design principle with biology and
chemistry to solve the most challenging tasks in the sustainability
revolution, such as, terminating the age of crude oil by using renewable
energy, feeding the world with minimum environmental footprints, powering
smart phones by metal-free batteries, etc. We propose to use in vitro
synthetic enzymatic pathways for low-cost biomanufacturing of a lot of
products from biof... 阅读全帖 |
|
发帖数: 1 | 21 你讲的有些方面很有道理，也很感谢你的建议，尽管有些观点我不能同意。
我做的是理论生物物理，因为觉得国内做这个的人太少了，所以我就把我的方向归为计
算生物了。
具体来讲，应该属于你说的第二种，但是我们更关注基因调控网络实现功能的物理机制
，而不是简单的解微分方程（解微分方程应该是与实验合作的做计算的人常用的手段）
。你说的一点很对，我的东西如果发给生物的评委，很可能他们不会喜欢。他们可能会
觉得没什么用。我的理解是，生物和物理是两种思维。我看到很多Cell上的文章讲的就
是他们实验发现了一个新的pathway 或者新的起作用的基因（对于某种功能，如癌症，
干细胞）。我不能说这样的文章不重要，因为从生物的角度，这都是第一次的发现。但
是从物理的角度，生物中得pathway 有很多，新的基因不断的在被发现。举个可能不太
恰当的例子，P53是癌症中得重要发现，但是人们能通过操控P53就解决癌症的问题吗？
不能，因为影响因素太多了，癌症的物理机制，它是为什么实现的并没有得到回答。这
个本质的问题是不能通过各种新的pathway，发现新的生物学细节得到回答。我们需要
生物中的“牛顿定律”。强调一下... 阅读全帖 |
|
d****5
发帖数: 202 | 22 回报本版，有意者请发信（附简历）到l***[email protected]联系。我也只能推荐，不能保证
能拿到审稿机会。
Title: Features of Large Hinge-Bending Conformational Transitions.
Prediction of Closed Structure from Open State
Abstract:
We performed a detailed analysis of conformational transition pathways for a
set of ten proteins, which undergo large hinge-bending-type motions with 4-
12 Å RMSD (root mean square distance) between open and closed crystal
structures. ANM-MC algorithm generates a targeted pathway between two
conformations, where ... 阅读全帖 |
|
n******i
发帖数: 374 | 23 您好，希望能把这次机会给我，谢谢了
Yarong Liu
2623 Ellendale Pl.
Los Angeles, CA 90007
(213) 249-2419
y*******[email protected]
www.linkedin.com/pub/yarong-liu/2b/a36/65b/
PUBLICATIONS
1. Yarong Liu, April Tai, Kye-Il Joo and Pin Wang. “Visualization of DC-
SIGN-mediated entry pathway of engineered lentiviral vectors in target cells
” 2013, PLoS One 8(6): e67400.
2. Liang Xiao, Kye-Il Joo, Yarong Liu, Jinxu Fang and Pin Wang. “
Injectable thermo-sensitive hydrogel as an adjuvant: in vivo modulation of
dendritic ... 阅读全帖 |
|
i********n
发帖数: 171 | 24 有审稿意愿的请发送你的CV到[email protected]/* */并告知你希望审稿的文章编号
请有时间愿意多审几篇的也可以，只要你能保证质量，之前有些审稿人有些敷衍，希望
大家能认真对待。谢谢合作。
1. Comparative assessment of cytotoxic effect of Cuban and Brazilian
propolis in human laryngeal epidermoid carcinoma cells
2. Role of PI3K/AKT/mTOR signaling pathway in ostearthritis by regulating
articular chondrocytes autophagy in rats
3. CREB knockdown inhibits growth and induces apoptosis in human pre-B
acute lymphoblastic leukemia cells through inhibition of prosurvival sig... 阅读全帖 |
|
l*********m
发帖数: 16971 | 25 WASHINGTON -- President Barack Obama said Wednesday that conversations
between the White House and members of Congress have begun over immigration
reform, adding that lawmakers would introduce a bill "very soon" into his
second term.
"This has not historically been a partisan issue," he said in his first
press conference since being reelected. "We've had President Bush, John
McCain and others who have supported comprehensive immigration reform in the
past. So we need to seize the moment."
It is ... 阅读全帖 |
|
b*****d
发帖数: 61690 | 26 Seven in 10 Americans don’t think Congress will be able to pass
comprehensive immigration reform, although Democrats and Hispanics are
slightly more confident, according to a poll released Friday.
Only 24 percent of registered voters think a comprehensive immigration law
will be signed this year, according to the Quinnipiac University poll, with
71 percent expecting negotiations between Democrats and Republicans to fail.
The full Senate is set to start considering the bipartisan Gang of Eight
le... 阅读全帖 |
|
b*****d
发帖数: 61690 | 27 Wisconsin Gov. Scott Walker (R) says he supports a pathway to citizenship
for immigrants living in the country illegally as part of an overhaul of the
nation's immigration system.
"If people want to come here and work hard and benefit, I don't care whether
they come from Mexico or Ireland or Germany or Canada or South Africa or
anywhere else," Walker said Tuesday during an interview with the Daily
Herald Media Editorial Board of Wisconsin. "I want them here."
Walker was then asked about the esti... 阅读全帖 |
|
b*****d
发帖数: 61690 | 28 Former President George W. Bush waded ever so gently into the fierce debate
in Washington over immigration reform, urging lawmakers to reach a "positive
resolution" on the issue, and warning against disparaging immigrants.
"We can uphold our tradition of assimilating immigrants, and honoring our
heritage of our nation built on the rule of law. But we have a problem. The
laws governing the immigration system aren't working; the system is broken."
Former President George W. Bush delivers remarks W... 阅读全帖 |
|
F*********n
发帖数: 2914 | 29 A good sign?
http://news.yahoo.com/top-us-republican-39-time-deal-39-immigra
Cambridge (United States) (AFP) - US House Speaker John Boehner gathered his
congressional Republicans Thursday to debate the party's future, saying the
time had come for his party to help pass immigration reform.
Secluded at a bayside hotel on Maryland's Eastern Shore for a three-day
closed-door retreat, the House of Representatives' majority party was
thrashing out policy principles, guidelines for the 2014 mid-term e... 阅读全帖 |
|
y******0
发帖数: 8807 | 30 ☆─────────────────────────────────────☆
oakleaf000 (George) 于 (Thu Jan 23 13:08:45 2014, 美东) 提到:
本人NIW批准在排队中。在MITBBS上感觉对移民改革讨论得不多，似乎是一件跟中国人
没啥关系的事情。有人认为移改是为非法移民度身订造的，就目前排队的一万多人来说真
是和1千多万非移没法相提并论，不过很显然，无论过去还是现在都不可能出现一千多
万合法非法的亚裔移民的情况的，所以，很奇怪为什么现在的EB2为啥不是很关注这趟
移改直通车，都能走EB1A吗？那还叫EB1A吗？现在的移改形势似乎趋于明朗，也略呈乐
观，估计非移公民之路绝了，但是身份可以合法，倒是即给共和党在西裔人前卖了好，
还坏了民主党票仓的大计，而民主党也没有铁定的理由完全否定共和党的做法，毕竟移
民法律的现状是崩坏的，美国经济复苏，用人在即，不能不算现实的压力。来自保守派
的势力很强，要保饭碗，谁都不想别人来抢饭碗，但是这个理由也不是很站得住脚，国
家发展总是要取最大利益的，就像上海当年想阻止外来人员抢饭碗，后来还是搞了开... 阅读全帖 |
|
y******0
发帖数: 8807 | 31 ☆─────────────────────────────────────☆
oakleaf000 (George) 于 (Thu Jan 23 13:08:45 2014, 美东) 提到:
本人NIW批准在排队中。在MITBBS上感觉对移民改革讨论得不多，似乎是一件跟中国人
没啥关系的事情。有人认为移改是为非法移民度身订造的，就目前排队的一万多人来说真
是和1千多万非移没法相提并论，不过很显然，无论过去还是现在都不可能出现一千多
万合法非法的亚裔移民的情况的，所以，很奇怪为什么现在的EB2为啥不是很关注这趟
移改直通车，都能走EB1A吗？那还叫EB1A吗？现在的移改形势似乎趋于明朗，也略呈乐
观，估计非移公民之路绝了，但是身份可以合法，倒是即给共和党在西裔人前卖了好，
还坏了民主党票仓的大计，而民主党也没有铁定的理由完全否定共和党的做法，毕竟移
民法律的现状是崩坏的，美国经济复苏，用人在即，不能不算现实的压力。来自保守派
的势力很强，要保饭碗，谁都不想别人来抢饭碗，但是这个理由也不是很站得住脚，国
家发展总是要取最大利益的，就像上海当年想阻止外来人员抢饭碗，后来还是搞了开... 阅读全帖 |
|
h*******g
发帖数: 994 | 32 谢谢热心同学的提供，继续上天入地的网上搜索收集资料中~ 我们一定会有个与众不同
的感恩节旅程的。
Sedona聖多娜，美麗的地理景觀比多數國家公園要亮麗壯觀許多，小鎮街道散佈精緻的
美國Old West傳統西部或是Adobe泥磚建築。Sedona曾被US Weekly的三百萬人問卷調查
中，票選為No. 1 Most Beautiful Place in America美國最美麗的地方。
Sedona 在New Age Movement新世紀運動扮演重要角色，是種新的生命觀哲學思想，New
Age導師Jose Arguelles在Sedona舉辦Harmonic Convergence聚會，認為Sedona有
Power Vortexes能量漩渦聚集，散發神祕磁場，此後眾多心靈療養場所蓬勃而生。
Sedona的Airport Mesa、Bell Rock、Cathedral Rock、Boynton Canyon四個紅岩地形
被New Age Adherents新世紀追隨者認定有Power Vortex能量漩渦，前二是Masculine
Energy男性能源，增加Spiritual S... 阅读全帖 |
|
a******o
发帖数: 16625 | 33 Business, Information Management and Marketing Career Pathway
Are you organized, accurate and self-motivated? Are you able to write and
speak well to communicate ideas to others? Do you enjoy being a leader,
organizing people, planning events and working with numbers or ideas? Do you
like operating computers or other business machines? This may be a career
pathway for you!
The Business, Information Management and Marketing Pathway includes the
following occupational clusters: administration and ... 阅读全帖 |
|
l*********m
发帖数: 16971 | 34 【 以下文字转载自 EB23 讨论区 】
发信人: lovefreedom (happy), 信区: EB23
标 题: lawmakers would introduce an immigration reform bill "very soon"
发信站: BBS 未名空间站 (Wed Nov 14 20:04:18 2012, 美东)
WASHINGTON -- President Barack Obama said Wednesday that conversations
between the White House and members of Congress have begun over immigration
reform, adding that lawmakers would introduce a bill "very soon" into his
second term.
"This has not historically been a partisan issue," he said in his first
press conference sinc... 阅读全帖 |
|
d**********u
发帖数: 4124 | 35 ☆─────────────────────────────────────☆
CaMKII (不作postdog) 于 (Thu Aug 20 12:39:18 2009, 美东) 提到:
发信人: CaMKII (不作postdog), 信区: Biology
标 题: 最新一期Cell两篇北大的文章（尚永丰&汤超）
发信站: BBS 未名空间站 (Thu Aug 20 12:38:55 2009, 美东)
http://www.cell.com/abstract/S0092-8674(09)00710-7
Cell, Volume 138, Issue 4, 660-672, 21 August 2009
doi:10.1016/j.cell.2009.05.050
LSD1 Is a Subunit of the NuRD Complex and Targets the Metastasis Programs in
Breast Cancer
Yan Wang1,Hua Zhang1,Yupeng Chen1,Yimin Sun1,Fen Yang1,Wenhua ... 阅读全帖 |
|
a*****n
发帖数: 40 | 36 我在加州，要申请license，发现还要一个CPA sponsor。
这个sponsor一定要是自己的supervisor么？还是其他认识的CPA就可以？
谢谢
Pathway 2
If you are licensed under this pathway, the majority of other states will
recognize your license. If you think that someday you might want to practice
in another state, Pathway 2 may provide you the professional mobility you
desire. Requirements:
A bachelor’s degree;
24 semester units in accounting-related subjects;
24 semester units in business-related subjects;
150 semester units (or 225 quarter units) o |
|
l**k
发帖数: 419 | 37
我反对的是你说的V1神经元可以用F变换来描述。因为V1结构比较清楚，
当然研究得是比较多。
研究LIP，是因为LIP是可能的注意中枢，而不是感觉和motor的整合。
另外，我认为你对视觉的研究理解有一定偏差。对ventral pathway是
modularity的确定。对dorsal pathway的研究是空间信息如何影响ventral
pathway和注意。其中眼动是一个热点。额叶主要是working memory。
你比我乐观啦。 |
|
l**k
发帖数: 419 | 38
我反对的是你说的V1神经元可以用F变换来描述。因为V1结构比较清楚，
当然研究得是比较多。
研究LIP，是因为LIP是可能的注意中枢，而不是感觉和motor的整合。
另外，我认为你对视觉的研究理解有一定偏差。对ventral pathway是
modularity的确定。对dorsal pathway的研究是空间信息如何影响ventral
pathway和注意。其中眼动是一个热点。额叶主要是working memory。
你比我乐观啦。 |
|
r****r
发帖数: 379 | 39 我很奇怪为什么连fly的凋亡都没有mitochondria pathway,而且mitochondria
pathway在体内的生理和病理情况下作用是否如culture cells里显示的那样重要
还很难说,比如bid,05年发现可以在核内起作用,有理由相信以后会有更多类似的发现.
所以,在更多的in vivo data出来之前,我对mitochondria pathway的重要性还带着疑问.
不过,尽管如此,我完全同意你的看法,XD的工作还是非常非常重要的,得不得nobel
prize已经显得不那么重要了. 这年头没得nobel prize的重要工作海了去了,得了
却不怎么让人觉得重要的东西也不少,前有mRNA的发现,后有EGF的发现.
Not sure I got ur points fully:))). But As far as I am thinking, there is
virtually no doubt whoever discover the mysteric enzyme(family?) will be a
very competitive contender |
|
n********k
发帖数: 2818 | 40 Yes, you are right, that's why I say specifically mammal(BTW, need to say
upfront, I am much less familar with non-verterbrate systems). Additionally,
although it doesn't seem Mitochondrial plays a central role but that doesn'
t deny MT pathway cause it is also called intrinsic pathway. Many players
are still there but may not reside in MT. And some people even suggest maybe
in fly, you don't need to cytochrome C release from MT cause it can
initiate the death pathway right at MT.
Regarding the |
|
r****r
发帖数: 379 | 41 我完全同意你的看法. MT的作用,应该不限于release pro-apoptotic factors,XD的主要
贡献在于release pathway的发现,当然还有extrinsic和intrinsic pathway之间的
crosstalk,不过我更欣赏MT作为一个unit在apoptosis中的作用.这样来说的话,release
factors只是
其中一种作用方式,XD如果当时接着去挖掘更多的MT作用,可能地位还会更高.
我记得他说过他当年思考过为什么进化会选择MT来调控apoptosis,可惜当时人们对MT
在apoptosis中的作用估计的太简单了,以为XD的工作一出来,就差不多搞定了,XD自己
估计也是这么认为,从inverterbrate animals来看,既然这些物种里面MT上很多蛋白在
不需要cytoc的情况下就可以调控apoptosis,那么有理由相信mammalian system里面这
些pathway
也会很重要,如果XD当时把这个也一举搞定,那就太牛了.
现在有些晚了,概念已经有了,剩下的就是细枝末节了.
Additionally,
doesn' |
|
p*****m
发帖数: 7030 | 42 呵呵 果蝇的behavior给整个neuro field贡献了学习记忆的cAMP pathway; 贡献了
circadian rhythm的基本整个mechanism; 贡献了几个重要signaling pathway的entry
point(e.g. MAPK-pathway)；甚至membrane potential的分子原理也可以算作behavior
贡献的 你再嘲笑
fly behavior很不公平的说 |
|
s******r
发帖数: 2876 | 43 MAPK难道不应该是yeast贡献的?
呵呵 果蝇的behavior给整个neuro field贡献了学习记忆的cAMP pathway; 贡献了
circadian rhythm的基本整个mechanism; 贡献了几个重要signaling pathway的entry
point(e.g. MAPK-pathway)；甚至membrane potential的分子原理也可以算作behavior
贡献的 你再嘲笑
fly behavior很不公平的说 |
|
r****r
发帖数: 379 | 44 现在aging领域的问题在于对aging的认识，actually aging is regulated rather
than controlled。这也是现在很多pathway做到下游就没法再按传统通路A--B--C的方
式做下去的原因。DR,我同意你的看法，算是一个影响因素，但不是什么多激动人心的
东西。reproductive system非常非常有意思，不过不抱什么期望怎么帮助aging研究摆
脱困境。这涉及到进化的问题了，到底aging是不是受进化选择。
有些人依旧按照传统pathway的方法往死了做，包括gary和canthia目前都有一部分工作
属于这类，其实意义已经不大了，这俩的徒弟，大部分都如此，可想而知就更惨了。
所以，我觉得andy很聪明，其实个人认为他最重要的发现不是他那些好看的cns通路文
章， 而是他几年前的science以及最近的cell的工作，涉及怎么将insulin 通路和
aging相关的疾病联系到一起，其实这些工作反应的是对aging的认识，自然也会影响到
genetic pathway在其中到底起什么作用：aging不是一个development |
|
t*d
发帖数: 1290 | 45 我现在在做这个，也在摸索。所以随便讲讲。
我们现在做的是比较treatment以后，基因表达的变化。microarray找出了好几百个基
因。所以我先用 GSEA 做了一个pathway/geneset analysis，看看那个pathway/
geneset 显著的变化了。然后从几个感兴趣，又显著的pathway/geneset中，找出那些
表达变化比较大的基因，用q－PCR，western，immunohistochemistry验证。然后和已
经发表的文献联系联系，扯上几句。比如A基因和细胞增殖有关，我的处理也和增殖有
关，但是A基因和我的处理的关系没有被报道，这样我在discussion里讨论讨论，把他
们联系起来。当然，如果条件许可，进一步做一些 gain of function 和 loss of
function 的实验，就更好了。做 GOF 和 LOF 比较费时，所以多看看文献，再决定哪
个基因的变化更可能有表型。这个时候你自己的 microarray 数据帮不上太多的忙，需
要你的专业知识，别人的报道，别人的 microarray 数据 （比如有人做过的有临床数
据 |
|
g**********y
发帖数: 423 | 46 我现在分析的是胃癌microarray gene expression data.
虽然胃癌本身具有多样性，但是和正常的对比，癌症样本区别还是很大的。
如果作为预测模型的话，准确率大概95%。
做pathway分析的话，有个奇怪的现象，那些oncogene, tumor suppressor相关的
pathway，并不是最高的或最低的存活率最低，反而是pathway activity在中间的样本
存活率最低，这个如何解释？ |
|
s*****t
发帖数: 71 | 47 我用一个动物模型，做了蛋白质组和代谢组，蛋白质组用的是metabolic label来定量
的，之后做了pathway的分析，但是得到的pathway基本之前都有报道和此病相关，但我
这里等于整合了一下，就是好多个pathway都显示发生了调控。
请问目前手里这些数据投molecular systems biology这个杂志是否合适？或者有更合
适的杂志可以推荐的？谢谢同学们。 |
|
g**********y
发帖数: 423 | 48 是这篇：A Genomic Strategy to Elucidate Modules of Oncogenic Pathway
Signaling Networks
另外，我总觉得他们用binreg(svd+probit reg)来预测pathway activity有个问题，就
是他们选的gene signature based on the correlation coeff between phenotype
and the gene exp。如果你仔细看看Bild 2006 的Nature paper，可以看出每个
pathway选出的gene list数目都在变，有时候75，有时候200，300等等，然后再作svd(
效果等同PCA)。这样的feature selection 到底对不对？
就像我们做clustering，只选top highly diff expressed genes做clustering可能有
问题，也可能没事？ |
|
y***i
发帖数: 11639 | 49 家族性的AD的变异一半以上是在r-secretae (Psn)上。而这个酶对amyloid plaque的
影响也非常清楚，所以这个酶和AD显然强烈相关，针对这个酶动手，就致病机理上是最
自然的事情，所以这不是个随便乱蒙的例子。现在没多少drug target比r-secretase更
清楚。也许你的总的论点是有道理的，但具体到这个r-secretae上，我想你举了错误的
例子。
唯一的问题是r-secretae是最重要的Notch pathway上必须酶之一。这个基因一旦彻
底敲除（把卵子mRNA也去掉），基本上什么都发育不出来，流产都免了。在果蝇胚胎发
育时大部分细胞都会变成神经细胞，是CPU级别的pathway。Notch pathway在成人中的
功能不完全清楚，但已经知道的部分也相当重要，比如免疫系统的维持。所以针对r-
secretae的毒性估计就从此而来。
amyloid
过。
plaque |
|
l**********n
发帖数: 240 | 50 Josef Martin Penninger
Most significant scientific contributions:
Our basic approach is to genetically manipulate and change genes in mice and
to
determine the effects of these mutations in development of the whole
organism and in
diseases. From these mutations we are trying to establish basic principles
of
development and basic mechanisms of disease pathogenesis. My laboratory
focuses on
heart diseases, autoimmune diseases and cancers, and bone diseases. On all
the listed
contributions below I ... 阅读全帖 |
|
