v********a
发帖数: 646 | 1 想做RNA/Protein的共定位
用ISH检测RNA,其中有一步是蛋白酶消化；
用IHC定位蛋白质, 其中有一步可能用到RNAse A消化；
请问哪位可以提供一个reliable的protocol，可以兼容RNA FISH/Protein IHC, 以此可
以看到RNA-Protein的co－locatization？
谢谢！ |
|
v********a
发帖数: 646 | 2 想做RNA/Protein的共定位
用ISH检测RNA,其中有一步是蛋白酶消化；
用IHC定位蛋白质, 其中有一步可能用到RNAse A消化；
请问哪位可以提供一个reliable的protocol，可以兼容RNA FISH/Protein IHC, 以此可
以看到RNA-Protein的co－locatization？
谢谢！ |
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x*******i
发帖数: 32 | 3 Hello There,
We have a protein (pI = 8.9, 10 mg/mL, MW 100 K) precipitated in 10 mM
phosphate buffer (pH around 7) after overnight storage at 4 degree. The
precipitate will be soluble again when warming up solution to room
temperature. Addition of NaCl will prevent precipitation. Other common
buffers are OK not causing precipitate.
Hypothesis is ionic cross-linking. Phosphate ion has intense negative charge
density and working as the cross-linking agent, its multiple negative
charges interact wi... 阅读全帖 |
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p****y
发帖数: 23737 | 4 【 以下文字转载自 Wisdom 讨论区 】
发信人: purity (purity), 信区: Wisdom
标 题: Protein: quality, not quantity, rules
发信站: BBS 未名空间站 (Wed Dec 2 02:42:36 2009, 美东)
http://www.penguin.ca/static/cs/cn/0/microsites/thrivediet/pdf/protein.pdf
The most alkaline-forming foods are those with chlorophyll, the green
pigment in many plants. Hemp is an excellent example in that it is contains
complete protein, yet the fact that it is not isolated and that it contains
chlorophyll helps maintain a more alkaline pH.
Dark types |
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j*******g
发帖数: 6 | 5 Hi guys,
I am working on establishing the model for two proteins based on one known
X-ray structure. I was able to create the model based on the primary sequence
of each target protein.
However, firstly I couldn’t use the known X-ray structure as template
directly, which might be because it contains 21 HETEROATOM. I don’t know how
to remove them. Alternatively I got the two proteins’ templates from EXPDB,
and merged them from two different layers to one layer using SPDBV, which
formed a heterodi |
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s****e
发帖数: 2934 | 6 If you just want to immobilizate protein in hydrogels, there are many
methods. Just search with protein and hydrogel, you can find many paper
about this area.
Basically,I guess you can mix protein together with the monomer and
hydrolysis. |
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m***n
发帖数: 266 | 7 想问一下: 这个microspheres和protein的质量或者体积比例
和protein release profile的关系,有什么已知的经验关系吗?
或者有已经文献?
一般都用microspheres和protein的质量比例,但是我感觉好象
真正关键的是体积比例, 是吗? |
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q*****8
发帖数: 23 | 8 诚心请教,有关peptide/protein 药的几个问题
1。原理？peptide/protein药不会被酶水解掉么?
2. 制药公司做这方面的多吗？
3。peptide/protein药前景如何？
谢谢了！！！！！！！！ |
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a***a
发帖数: 40617 | 9 【 以下文字转载自 Fitness 讨论区 】
发信人: anoia (暂时解决温饱,坚决不进器材), 信区: Fitness
标 题: 推荐一个牛奶的替代品（早餐，冲protein shake）
发信站: BBS 未名空间站 (Mon Jul 27 19:25:41 2009, 美东)
Almond Breeze
就是杏仁露了，一般美国超市卖牛奶的旁边就有
unsweetened的一个240ml只有40cal（同样体积的skim milk有90 cal）
缺点是protein含量比牛奶低很多（1g vs 10g）
但是热量低了一倍多，口感比skim milk好（我加splenda喝或者对protein shake）
而且其他乱七八糟营养成分都比牛奶多，比如
（1 serving，240ml）
Vit A 10%
Calcium 30%
Iron 4%
Vit D 25%
Vit E 50% （妇女同胞最爱？）
Riboflavin 2%
Magnesium 4%
Zinc 2%
Copper 4%
如果不想喝没甜味的，那么有甜味的，每个servin |
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p****y
发帖数: 23737 | 10 【 以下文字转载自 Wisdom 讨论区 】
发信人: purity (purity), 信区: Wisdom
标 题: Protein: quality, not quantity, rules
发信站: BBS 未名空间站 (Wed Dec 2 02:42:36 2009, 美东)
http://www.penguin.ca/static/cs/cn/0/microsites/thrivediet/pdf/protein.pdf
The most alkaline-forming foods are those with chlorophyll, the green
pigment in many plants. Hemp is an excellent example in that it is contains
complete protein, yet the fact that it is not isolated and that it contains
chlorophyll helps maintain a more alkaline pH.
Dark types |
|
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w*******y
发帖数: 60932 | 12 Hey guys, i have been looking for some good, cheap WHEY protein and have
come across some EAS Myoplex 5lb whey protein for the BEST price of $20.14
Shipped w/ Freebie (i chose vics cough drops)
Okay, so here are the steps to getting the protein, its Chocolate or Vanilla
...
1) Go to this link: Link:
http://www.soap.com/product/productdetail.aspx?productid=62151
2) Add the Quantity of either chocolate / Vanilla to your order
3)(a) add anything else over 1 cent to your cart that you would like to |
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w*******y
发帖数: 60932 | 13 Amazon
Worldwide Pure Protein High Protein Bar, Chocolate Peanut Butter, 2.75-Ounce
Bars (Pack of 12)
$14.09 - 15% off Sub & Save
= $11.98 with free shipping
Note, you may cancel your subscribe & save subscription any time after your
order ships.
|
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w*******y
发帖数: 60932 | 14 I bought this about two weeks ago. I saw that someone else had posted a
pretty good deal for whey protein from somewhere else and I realized that
the deal I got a few days ago beat that one (I think).
You get (3) 2lb tubs of whey protein and you get to mix and match the
flavors, all for $35 and FS.
Link:
http://www.walmart.com/ip/Body-Fortress-Whey-Protein-Powder-Val
Enjoy!
|
|
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w*******y
发帖数: 60932 | 16 Special K Protein Meal Bar, Strawberry, 6-Count 1.59 oz. Bars (Pack of 3) $6
.37 free shipping from Amazon
Use Subscribe and Save to get price and Free shipping
Special K Protein Meal Bar, Strawberry, 6-Count 1.59 oz. Bars (Pack of 3):
http://www.amazon.com/Special-Protein-Meal-Strawberry-6-Count/d
$6.37
|
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w*******y
发帖数: 60932 | 17 First post for me. Didn't find this deal posted.
Amazon.com has 2x2lbs of Optimum Nutrition 100% Natural Whey Protein (
Natural Chocolate) for $20.56 after S&S.
You may cancel your subscription at any time or after order has been shipped.
This comes to a cost of $0.32 / oz per order. Very good for natural whey
protein isolate / concentrate mix. No extra fillers, etc.
Optimum Nutrition 100% Natural Whey Protein:
http://www.amazon.com/Optimum-Nutrition-Standard-Natural-Chocol
|
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w*******y
发帖数: 60932 | 18 Special K Protein Meal Bar, Strawberry, 6-Count 1.59 oz. Bars (Pack of 3) $6
.37 free shipping from Amazon
Use Subscribe and Save to get Price and Free shipping
Special K Protein Meal Bar, Strawberry, 6-Count 1.59 oz. Bars (Pack of 3):
http://www.amazon.com/Special-Protein-Meal-Strawberry-6-Count/d
$6.37
|
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w*******y
发帖数: 60932 | 19 Link:
http://www.amazon.com/Optimum-Nutrition-Casein-Protein-Vanilla/
Amazon has Optimum Nutrition 100% Casein Protein in the French Vanilla
flavor available as a 2-pack of 2 lb. containers for $31.81, and then it
drops down to $27.04 after Subscribe and Save. Shipping is free, so it comes
out to $6.76/lb. of casein protein, which is a good deal.
|
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w*******y
发帖数: 60932 | 20 Link:
http://www.amazon.com/Twinlab-Protein-Chocolate-Surge-Pound/dp/
$30 for 5 lbs of any protein is good, but I'm currently using this exact
protein and it taste good. Dymatize is my favorite, but I can't find it for
this price.
I also have a bag of Mustletech chocolate here and it tastes like shit.
|
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w*******y
发帖数: 60932 | 21 It is not the best price ever for this whey protein but the lowest recently.
Cheap but quality protein. I tried ON and this, both chocolate favor. I
like this more than ON personally.
Link:
http://www.amazon.com/Twinlab-Protein-Chocolate-Surge-Pound/dp/
Click subscribe and save to get the discount price.
|
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w*******y
发帖数: 60932 | 22 **READ! STARTS 11/16, I'm pretty sure there'll be a coupon code posted by
them by the morning for this deal so this is a heads up only till it goes
live.
Once its up i'm sure someone will post it.
Deal is for 2 * 5lb Muscle Gauge whey protein powder! They always got pretty
good reviews so unless ur real picky, definitely sounds like a hot buy for
protein these days. Recession price...shipped free, no brainer. I'm
personally getting a couple tubs to store as my emergency stash.
Link posted for r... 阅读全帖 |
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w*******y
发帖数: 60932 | 23 5.04-Pound Tubs BSN Syntha-6 Protein Powder Strawberry Milkshake $33.47,
Chocolate Peanut Butter $34.42 Free shipping from Amazon
Use Subscribe and Save to get Price and Free shipping
BSN Syntha-6 Protein Powder Strawberry Milkshake $33.47
BSN Syntha-6 Protein Powder Chocolate Peanut Butter $34.42
|
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w*******y
发帖数: 60932 | 24 Vitacost has their "Vitacost Whey ISO Protein Isolates Chocolate -- 3 lbs:
http://iway.org/9587711
" on sale for buy one get one 50%, just add it to cart and another one will
be added for half the price.
If you do self-referral through vitacostrewards.com [vitacostrewards.com]
you get a $10 off coupons.
Here is the math...
Vitacost Whey ISO Protein Isolates Chocolate -- 3 lbs (1,362 g) 1 $
29.99 $29.99
Item No: NSI 3003914
Vitacost Whey ISO Protein Isolates Chocolate -- 3 lbs... 阅读全帖 |
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x****6
发帖数: 4339 | 25 . The binding free energy between the Wuhan CoV S-protein and human ACE2 was
–50.6 kcal mol–1, whereas that between SARS-CoV S-protein and
ACE2 was –78.6 kcal mol–1.
基于计算的 测量，绝对值没有相对值有意义 |
|
ik
发帖数: 167 | 26 之前学者研究新冠肺炎的致病机理，得出的结论是 S-protein和人类ACE2 蛋白特异性
结合，造成感染
但从目前 10万例感染病例，和将近 3千死亡病例看，不同人的感染程度，症状轻重
截然不同。
因此我推测，新冠病毒的致病机理，可能不只 ACE2一个受体，或者 S-protein+ACE2
受体一个通路，可能还存在其它没有被发现的途径，有待证实。
------------ |
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f*******0
发帖数: 62 | 27 有幸得到一个AP position， 现需要估算个 startup package。 因为我主要是用E.
Coli express and purify membrane proteins，特向有经验前辈们请教几个主要用到
的仪器大概价格 （有个区间就行）。多谢多谢！
1. High performance liquid chromatography
2. 1L bacteria incubation shaker
3. OD spectrometer (for bacteria concentration measurement)
4. Allegra type centrifuge (for protein solution centrifuge／concentrate)
5. Bacteria cell pelleting, harvesting centrifuge
6. ultracentrifuge
7. Benchtop microcentrifuge
8. mechanical bacterial cell breaker
9. UV absorption spectr... 阅读全帖 |
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f*******6
发帖数: 665 | 28 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: flora6866 (Flora), 信区: Pharmaceutical
标 题: 推荐个protein formulation summer intern职位
发信站: BBS 未名空间站 (Fri Apr 23 17:51:57 2010, 美东)
我一个哥们儿直接招summer intern
最好是chemical engineering, biochemistry, chemistry or related 在校学生
summer intern，有可能会延长，公司提供travel和住房补助，decent salary
希望有hplc, UV, particle sizing, HIAC 等 hand-on experience，能有protein的背
景加分.
有兴趣的发信到t*******[email protected]
机会宝贵，抓紧时间哦！ |
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m****a
发帖数: 85 | 29 【 以下文字转载自 SanFrancisco 讨论区 】
发信人: msexta (抠脚大汉), 信区: SanFrancisco
标 题: 求推荐湾区附近protein science相关工作
发信站: BBS 未名空间站 (Sun Aug 7 12:14:05 2016, 美东)
PhD马上毕业，有不少一二三作paper，另有公司实习相关经历，希望年底毕业后能去湾
区附近工作（朋友在湾区工作机会多，暂时未毕业，所以打算我先过去）。方向是
molecular biology and biochemistry，主要做蛋白相关的课题，包括构建，表达，纯
化，酶活力检测，质谱分析等，免疫的技术都有过相关经验。希望找protein science,
enzymology, antibody and immunology相关的工作。投过很多简历到各种大小公司，
大都杳无音讯。
湾区附近的生物公司很多，希望看帖的各位能帮帮忙！感激涕零！ |
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t*******n
发帖数: 4445 | 30 Most protein supplements come from soybeans or whey. The major factors are
* inclusion of all essential amino acids
* matching of amino acid composition to normal bodily need
* taste, digestibility
In addition, soy protein isolates have some additional reported health
benefits (control of cholesterol and osteoprosis)
It's important not to overdose or become dehydrated. Your brother should
probably consult his doctor first, too. |
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R******d
发帖数: 1305 | 31 what about protein powder or protein bar? |
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R******d
发帖数: 1305 | 32 enn I had a big protein bar today 30g of protein haha |
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c****c
发帖数: 169 | 33 实在是不够可以吃PROTEIN SHAKE.注意要TOTALLY ORGANIC的. JAY ROBB 的是孕妇可以
吃的. 稍微贵一点. 补充PROTEIN很重要.不要听中国OB说50-60就好. |
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t****r
发帖数: 7 | 34 求审稿，
protein physical chemistry，食品方向的protein，抗菌，delivery system
站内联系。
：） |
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t*******y
发帖数: 4 | 35 办绿卡用
关键词： chemical biology, protein chemistry, heme protein, enzymatic
catalysis, bioinorganic/biophysical chemistry, bio-organic chmistry
已经审过2篇杂志，站内联系，提供cv
大家多帮帮忙！ |
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k******e
发帖数: 8870 | 36 Updated: a recommendation has been sent.
a RSC journal invited me to review a manuscript on Hsp90, especially the n-terminal domain. Because i am so busy recently, i would like to recommend someone here to review it. you will need to have some background on protein protein interactions,enzymology, as well as computational structural modeling. if you have the expertise in this field and are interested in reviewing the manuscript, please pm me with a brief description of your background (includin... 阅读全帖 |
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s***y
发帖数: 24 | 37 guest editor机会.
Journal: Biochemistry insights journal supplement (Libertas Academica)
Scope: Protein binding and protein interactions
Tasks: 1. Solicit five papers
2. Compose an editorial
如果有兴趣，请站内联系。 |
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i*n
发帖数: 9793 | 38 我们公司正在招人，要求本科或者硕士学位，有两年做protein HPLC (SEC, RP-LC)工
作经验，掌握基本的protein/antibody实验室技术。如果哪位有兴趣，请先站内联系。
谢谢！ |
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k****h
发帖数: 944 | 39 I argued in an earlier post that protein should not be taken right before
sleeping and was countered by another post. Here comes my understanding.
Protein metabolism generates ammonia, a by-product that is toxic to our
body. Our body must detoxify it to a form that removed from the body. Thus,
ammonia is converted to urea, which is water-soluble and is readily
excreted via the kidneys in urine. To flush out this byproduct necessiates
the consumption of a lot water, which helps excrete the ammon |
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d*i
发帖数: 9453 | 40 ☆─────────────────────────────────────☆
wildMerth (Rapunzel! Let down your hair!) 于 (Sat Nov 17 22:54:13 2007) 提到:
只跑了6唛, 大约是因为中午只吃菜没吃饭的缘故. 跑前加了一块protein bar, 按说明
, 也有330大卡 (其中110大卡来自fat), 也喝了足够多的水, 但就是没力气. 不知道怎
么回事. 汗水倒是掉了快两磅.
☆─────────────────────────────────────☆
mark (花生，微爷远爷的爸爸) 于 (Sat Nov 17 22:56:15 2007) 提到:
跑步前应该carb. not protein/fat .
☆─────────────────────────────────────☆
hawking (丝绸之路～月牙泉水) 于 (Sat Nov 17 22:57:22 2007) 提到:
bar很好吃。我很喜欢吃巧克力和花生酱
☆────────────────────── |
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c*****9
发帖数: 26 | 41 我一直吃GNC自己店出的 WHEY PROTEIN 巧克力味道的 还不错
6LBS 35.99
大家还有性价比更好 或者 价格别太高的 好点的WHEY PROTEIN推荐吗??
谢谢了 Orz |
|
d*i
发帖数: 9453 | 42 ☆─────────────────────────────────────☆
freelong (不够时间好好来恨你) 于 (Tue Feb 19 11:23:24 2008) 提到:
For the normal daily food we take,
how much we get from them?
50g, 60g?
I want to make sure how much protein shake I need to supply. (1 serving, 24g
protein)
☆─────────────────────────────────────☆
ostrichgege (空心菜) 于 (Tue Feb 19 11:28:57 2008) 提到:
我记得是每天要35g....Normal daily food这个很难说啊,一哥的Normal跟俺的Normal
差的远了去了....你说说你每天都吃啥了.
24g
☆─────────────────────────────────────☆
hillcrest (5K) 于 (T |
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s*****w
发帖数: 2201 | 43 calorie ， carb 、 protein 每天应该吃多少？
应该按什么标准吃？
分开说说
eg：、
要减fat的怎么吃，
维持体重的？
增加muscle的？
听说是
protein要吃：g数是 体重磅＊（１－body fat％）
不知道对不对？这个是针对什么情况下的呢？
其他的呢？
谢谢 |
|
S*********g
发帖数: 7653 | 44 我感觉没事
我买了一箱Protein bar,每次长距离跑（5mile-7mile）之前吃一块，250卡，既然是
Protein bar,碳水就是比较少的，好像是10g，蛋白质32g，脂肪也很少，2g以内。这个
东西比较抗饿
我一个学生推荐我Clif bar, 这个碳水比较多45g，蛋白质22g，不同口味的都有，减肥
的话还是要控制碳水吧
40分钟有氧：40分钟跑步？大概能跑6mile吧，差不多消耗快800卡（150lbs) |
|
e*****e
发帖数: 2233 | 45 Age 18个月以上的, 否则不够硬, 狂鲜(想象一下放很多味精的粥的味道)
Protein高达40%快赶上Whey Protein了(52%左右), 但是味道一个
天上一个地下
不好的是fat多偏咸(cheese通病, 不敢当饭吃.
Party常常当点心serve
今天去costco找前两天版上提到的cottage cheese,
greek yogurt都没有找到但是找到了这个, 也不知道是否正宗Italy import,
但我吃不出区别来
睡前再来一口, wahaha... |
|
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d*******d
发帖数: 1341 | 47 我以前吃过一种蛋白含量比较高的Whey Protein，还是有口味的，已经腥气很大，吃下
去挺困难了。不知道UNFLAVOURED Whey Protein的味道会不会因为制作工艺什么的还可
以忍受？有哪位吃过，能不能分享一下经验 |
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D*D
发帖数: 20 | 48 costco里基本就三大种，cytosport的muscle milk，cytosport的whey protein和
optimum nutrition的whey protein。大家一般吃的哪种？ 我有点担心cytosport的产
品据说heavy metal都超标，大家有啥经验和建议吗？谢谢 |
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b********1
发帖数: 308 | 49 Vascular effects of a low-carbohydrate high-protein diet
Communicated by Bruce M. Spiegelman, Harvard Medical School, Boston, MA,
July 17, 2009 (received for review May 15, 2008)
The cardiovascular complications of obesity have prompted interest in
dietary interventions to reduce weight, including low-carbohydrate diets
that are generally high in protein and fat. However, little is known about
the long-term effects of these diets on vascular health. We examined the
cardiovascular effects of a lo |
|
s*****w
发帖数: 2201 | 50 应该按什么标准吃？
分开说说
eg：、
要减fat的怎么吃，
维持体重的？
增加muscle的？
听说是
protein要吃：g数是 体重磅＊（１－body fat％）
不知道对不对？这个是针对什么情况下的呢？
其他的呢？calorie ， carb 、 protein 每天应该吃多少？
谢谢 |
|
