r*******1
发帖数: 2026 | 1 我爸妈,
这岁数有点肌肉挺好的
但我爸有糖尿病。好像有服药。
我妈劲颈椎不好,没服药
因为不懂,protein powder不知道有啥注意不,对于老人 |
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F******a
发帖数: 562 | 3 吃多了都胖，nuts一小把200卡，protein bar你可以看看quest bar |
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b*****l
发帖数: 8603 | 4 belvita biscuits
newtons fruit thins
builder's 20g protein bar(chocolate mint is the best)
主要是口味好，热量都不低，吃什么吃多都会胖，少吃就不会。 |
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b********r
发帖数: 620 | 5 本人奔4大叔，比较胖，而且有家族糖尿病史，因此今年4/5月开始减肥。2月左右减去
了20磅，BMI从29+减到了25+，有少许反弹，现在BMI在26左右，还没有完全正常。血糖
正常。主要是跑步加控制饮食。现在大概每周跑2/3次，跑步机上大概7.2m/h，大概跑4
~5mile。
现在想进一步减肥增肌，主要是考虑肌肉可以更好的帮助控制血糖，自己小的时候也比
较喜欢健身。不知道这种情况推荐用那种meal replacement蛋白粉不？http://www.gnc.com/Protein/Meal-Replacements/family.jsp?categoryId=12950051。就是有一定卡路里，每次锻炼前（我一般午饭前跑）2杯，之后不再吃饭。这种东西会让你跑步哑铃耐力更好吗？也就是说可以让你跑更快些更久些？这样同样的时间锻炼效果更好？
大家都用这些东西吗？有没有可以推荐的牌子？ |
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a******1
发帖数: 1519 | 6 60% carbo, 35% protein and 5% fat. |
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c****d
发帖数: 1347 | 7 我上星期刚买了一大盒granola bar
一个140卡，carb 20g，fat 5g, protein 6g
回家才发现sugar 6g.............极其不爽，
哎，不过这几天每天都有吃一个。。。 |
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y**d
发帖数: 2993 | 8 比Pure Protein bar味道好多了，就是贵了点 |
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j*********r
发帖数: 24733 | 9 靠我刚order了Pure Protein bar. |
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j*********r
发帖数: 24733 | 10 谢谢, 我得先把Pure Protein bar吃完再说. |
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w**********g
发帖数: 2077 | 11 Naked Protein Juice Smoothie
效果责任自负，也许俺看到Naked就喜欢，心理作用 |
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h****w
发帖数: 1363 | 12 谢姚明信息，接双黄包子。
我以前就是比赛后抓几个protein bars留着慢慢吃，吃完就没了。实际应该每次hard
run后都吃(?)。
这就去Amazon买一盒Quest。 |
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R*s
发帖数: 2041 | 14 【 以下文字转载自 Biology 讨论区 】
发信人: biospiner (biospiner), 信区: Biology
标 题: Re: 请问protein sequence alignment怎么做？
发信站: BBS 未名空间站 (Sun Nov 5 00:16:09 2006)
http://www.ebi.ac.uk/clustalw/index.html |
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a**o
发帖数: 1470 | 15 给我家这个8月大的puppy挑粮食哩。protein不能太高，不知道30%算不算太高？ |
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d*******s
发帖数: 15155 | 16 哪些是中国狗粮啊？坚决不买，怕protein都是三聚氰胺抬上去的 |
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g***m
发帖数: 465 | 17 hehe, it is a basic strategy via accurate MS for proteomic level's screening
and identifying.
The precision is really dependant on how you construct the peptide map
database. Generally, they take cDNA translated amino acid sequence as actual
proteins, then do a in silica proteolysis with trypsin, input the results into
the database.
This approach is really database dominating. Not mention possible
post-translational modification and signal peptides and so on that could bring
a big trouble. |
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g***m
发帖数: 465 | 18 a group from Zurich found protein phosphotase 1 (PP1) is involved in forgetting.
quite interesting, their experiments are mainly based on hehavior research using
transgenically PP1-inhibited mice.
It tells, longer breaks give better memory. As a result, have a regular break
when you are cramming something is really good for memorizing, hehe. |
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m*******h
发帖数: 896 | 19
160 Da difference ?
N-terminal phosphogluconoylated: (178 Da 258 Da difference)
Anal. Biochem. 1999, 267 169-184
M-terminal mythylation:
Protein Sci. 1995 , 4, 2616-2618 |
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r****o
发帖数: 105 | 20 1.比较容易纯化，用protein A agarose即可
2.有利于分泌表达。
3.稳定被表达的蛋白，Fc可以起类似分子伴侣的作用。
4.如果这种蛋白是药用，Fc可以提高蛋白在人体内的半衰期。 |
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r****o
发帖数: 105 | 21 I guess it is due to the residue of ethanol. Use a speed-vac to
dry down the protein pellet completely or incubate the tube with
the sample at 37C for hours. |
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l*****m
发帖数: 98 | 22 i am looking for postdoc position. is protein crystallography a good area?
thanks! |
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s******y
发帖数: 28562 | 23 I think Protein A binds antibody naturally. |
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a****o
发帖数: 1786 | 24 IgG.
That's why protein A-agarose beads are used to pull down IgG antibodies in
ChIP experiment. |
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S***l
发帖数: 323 | 25 definitely histone protein |
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l*******6
发帖数: 128 | 26 Hello, everyone,
I have difficulty in Co-IP two chromatin-bound proteins (in vivo) using
regular IP protocol. Could anyone
recommend a protocol that is optimized for this purpose, or give some
suggestion?
Thank you so much! |
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n**********1
发帖数: 251 | 27 最近要做mRNA-Protein co-Immunoprecipitation，对于大致的步骤和原理大概知道
，不知道有没有相应的试剂盒，还有在沉淀下来后，为什么要加入yeast tRNA，或者
linear acrylamide 和 glycogen，据说作用是carriers，我不太知道具体是什么作用
？ |
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w******e
发帖数: 1187 | 28 有没有价格便宜量又足的地方？要用很大量的protein，查了下millipore，每
25ug 300刀，太贵了。。。 |
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w******e
发帖数: 1187 | 29 thx for the input. I did that too. but when I tried to verify it
by directly measuring the proteins on beads, the result didn't add up... |
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p******n
发帖数: 61 | 30 try Bio-Rad affi-gel-10 or affi-gel 15 (dependent on protein pI). |
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w******e
发帖数: 1187 | 31 要把买来的purified protein从一种buffer换到另一种buffer
现在用amicon YM-10，recovery rate70％左右。算正常吗？有没有
recovery 更高的column？多谢！ |
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s******y
发帖数: 28562 | 32 It's normal that the protein order from the company may have precipitation
or denatured fractions during storage. Those will stuck in the resin.
Therefore, a 70% recovery rate is not bad.
P.S....."千老之王"...Me Ft... |
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s******y
发帖数: 28562 | 33 Do you mean the primary structure (protein sequence)?
Or the exact crystal structure? (3rd structure)?
Either way you can find them on NCBI database |
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p*****c
发帖数: 1506 | 34 在purified protein上做biotin或streptavidin标记,非anti-his抗体的
欢迎提供相关信息
多谢 |
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F*****y
发帖数: 23 | 35 Hi Is there any one going to attend the workshop of protein immunogenicity
and aggregation at Colorado from Jul 19 to Jul 22? I hope to find a female
to share a hotel room as there are two beds for each room. People can only
book one whole room as reservation.
Please contact j*******[email protected]
Thank you! |
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u*****e
发帖数: 357 | 36 请问什么PROTEIN 浓度可以直接用ABSOBANCE 读出来? 谢谢! |
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m**z
发帖数: 787 | 37 depending on the Trp and Tyr content of the protein sequence. you can use
protparam to calculate the extinction coefficient, then calculate what
concentration will give you an OD above 0.1 |
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s*********r
发帖数: 13 | 38 第一次设计fusion protein,想给自己的蛋白加个mcherry tag,没有现成的载体，不知
道中间需要加个linker呢，还是直接按ORF接上就好呢？pcDNA3.1的vector。没有现成
的linker给我用，如果要加的话，是不是得设计到primer上去，那这primer也太长了吧
，能P出来吗。。。。
谢谢指导^^ |
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n***w
发帖数: 2405 | 39 我正在做一个fusion protein，我就是设计引物，然后p。。。引物大概35个碱基吧。
。。overlap。。。还是比较好p的。。。 |
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s******y
发帖数: 28562 | 40 NO, I mean using 2M tris to disrupt the binding of protein but not the
binding of DNA or biotin-avadin
1M |
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a****o
发帖数: 1786 | 41 Did you use agarose or polyacrylamide gel?
Try to add some BSA and tRNA as nonspecific competitors.
Does your protein tend to form oligomer?
You can also try to add some DTT. |
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w******e
发帖数: 1187 | 42 Thanks for the info. but protein alone wouldn't have signal since it's
not labeled |
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p******n
发帖数: 20 | 43 用cell lysate然后IP不能说明是不是direct interaction吧？ 是不是只能说明两个
protein在一个complex里？如果要证明direct interaction有什么办法呢？谢谢！ |
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b**j
发帖数: 415 | 44 putattive sequence, XXKXKXKRKR, or something about that?
you can also find some NLS seq from other protein to do an alignment |
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g***1
发帖数: 24 | 46 Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences! |
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i********e
发帖数: 50 | 47 I use 6% separation gel and transfer without methanol for one hour at 100 V
. My protein is 550 KDa. |
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s******r
发帖数: 2876 | 48 invirogen 有 Tis-acetate gel，据广告适合大蛋白。
Does anyone have experiences in western blot handling high molecular weight
(250~360 kDa) proteins? I met difficulties in transfer, thank in advance for
sharing your experiences! |
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