g**p
发帖数: 10 | 1 有个protein，比如叫XYZ吧，在做alignment的时候它的mature protein 应该标为mXYZ
,还是XYZm? |
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r******g
发帖数: 600 | 2 有full length，untagged 的对照～ 也有full length, tagged的对照～ molecular
weight没有问题
我没有克隆 untagged的 N-ter truncated protein 或者 C-ter trucated protein,
另外，没有争对 C-ter truncated region的 抗体
唯一的抗体 是对Nter的epitope |
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M******g
发帖数: 152 | 4 关键字: protein categorization (gene ontology (GO) analysis
发信站: BBS 未名空间站 (Wed Apr 6 13:49:28 2016, 美东)
Can anybody recommend a good software or server to do protein categorization
(gene ontology (GO) analysis)?
Thank you. |
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m****a
发帖数: 85 | 5 【 以下文字转载自 SanFrancisco 讨论区 】
发信人: msexta (抠脚大汉), 信区: SanFrancisco
标 题: 求推荐湾区附近protein science相关工作
发信站: BBS 未名空间站 (Sun Aug 7 12:14:05 2016, 美东)
PhD马上毕业，有不少一二三作paper，另有公司实习相关经历，希望年底毕业后能去湾
区附近工作（朋友在湾区工作机会多，暂时未毕业，所以打算我先过去）。方向是
molecular biology and biochemistry，主要做蛋白相关的课题，包括构建，表达，纯
化，酶活力检测，质谱分析等，免疫的技术都有过相关经验。希望找protein science,
enzymology, antibody and immunology相关的工作。投过很多简历到各种大小公司，
大都杳无音讯。
湾区附近的生物公司很多，希望看帖的各位能帮帮忙！感激涕零！ |
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x****6
发帖数: 4339 | 6 来自韩在addgene提供的protocol
*Since NgAgo follows “one-guide faithful” rule, i.e. guides can only be
loaded when NgAgo protein is in the process of expression, to improve the
efficiency of gDNA loading to NgAgo, sometimes multiple transfection of gDNA
helps
"guides can only be loaded when NgAgo protein is in the process of
expression",如果真是这样，这个蛋白也是蛮奇葩的。 |
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l*****m
发帖数: 98 | 7 Hi,
I am a protein biochemist and furthermore structural biologist. I have long
experience in protein biochemistry, biophysics, and crystallography. I am
very interested in the position. Can you please let me know how I can apply
for the position? My email is [email protected]
Thanks. |
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k***i
发帖数: 662 | 8 August 9, 2007
BIOCHEMISTRY
Bent DNAs Help Probe DNA-Protein Binding
New class of artificial DNAs could help assess structure-specific DNA recognition
Celia Henry Arnaud
Many DNA-protein interactions involve structural distortions of the DNA helix. A new class of artificial DNAs, called "modular bent DNAs," could help systematically assess the role that DNA bending plays in these interactions, according to a recent study (J. Am. Chem. Soc., DOI: 10.1021/ja071335k).
2′-Deoxyuridylate dimer, n = 1 |
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m******m
发帖数: 13 | 9 A postdoctoral research position funded by a four-year NIH grant is
immediately available in Dr. Jianlin Cheng's group in the Department of
Computer Science and Informatics Institute at the University of Missouri,
Columbia. The scientist at this position will work on a project to design,
develop, and benchmark cutting-edge computational methods and tools to
predict protein structures from protein sequences. The methods and tools
will be tested in the biannual world wide Critical Assessment of Te |
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b*******g
发帖数: 1309 | 10 方法：QCM SPR 比较常见
带荧光的protein，可以搞GFP 这类的proteins
或者自己用ｄｙｅ修饰ｐｒｏｔｅｉｎ
一般用BSA比较多，便宜量有足，容易修饰 |
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f****z
发帖数: 47 | 11 可以参考NP表面修饰DNA的测定方法。跑胶，或者用带荧光的protein，然后做
bleaching。 |
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t********y
发帖数: 749 | 12 【 以下文字转载自 Biology 讨论区 】
发信人: trajectory (trajectory), 信区: Biology
标 题: 外行问一个transmembrane protein的实验问题
发信站: BBS 未名空间站 (Tue Mar 15 15:36:48 2011, 美东)
在membrane里面放某种transmembrane protein， 怎么知道放的是正的还是反的呢？
还是两种orientation 都有呢？
特别是PH sensitive channel
谢谢！ |
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x*******i
发帖数: 32 | 13 Hello there:
Trytophan fluorescence spectroscopy could be used to analyze whether protein
is compact or open. Ex at 288 nm and em around 320 nm suggests that protein
is compact (trytophan in hydrophobic environment). Em at longer wavelength
suggests an open structure. Also relative fluorescence intensity could tell
something? Anybody know why?
Thanks |
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U**********b
发帖数: 13 | 14 The Li lab in the Department of Chemistry at University of Louisville is
seeking a highly motivated postdoctoral researcher to join our lab
immediately.
Our research interests focus on applying solution NMR techniques to
understand the functional roles of protein conformational dynamics in the
deubiquitinase family. We’re especially interested in deubiquitinases that
are involved in signaling pathways implicated in cancer and autoimmune
diseases. To accomplish our research goals, we complement s... 阅读全帖 |
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m****g
发帖数: 118 | 15 实验所需，要买一些 purified soy proteins (glycinin and beta-conglycinin)。
可是用了很多方式查找(包括google),都找不到哪里有卖的信息。
Please give me some hints if you happen to know where I may get some of these
proteins, and any suggestion would be highly welcome!!!
Many thanks!!! |
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s*****y
发帖数: 27 | 16 is there anybody who is learning protein dynamics ? and protein folding and
binding something like that . |
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m***n
发帖数: 266 | 17 realeagle你的回帖我用telnet看得见，但是www方式看不见？
我的想法是：包在里面的protein release是和周围有多少组成microsphere
的分子有关．如果是质量比例，假设换了体积很大或者很小的protein，总质量
不变，那么释放速率应该会受到影响吧？ |
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r*******e
发帖数: 83 | 18 这个释放速率的问题不同蛋白间本来就很难比较，换算成体积含量似乎也不能解决问题
。再者不管是动物实验，还是后续临床应用，都会涉及剂量给药，采用质量百分数便于
换算。你如果去考虑一个蛋白分子周围polymer的分子数，只会把问题复杂化。不同分子
量或者分子体积的蛋白间释放速率的差异始终存在，不管你是否考虑质量含量还是体积
含量。
realeagle你的回帖我用telnet看得见，但是www方式看不见？
我的想法是：包在里面的protein release是和周围有多少组成microsphere
的分子有关．如果是质量比例，假设换了体积很大或者很小的protein，总质量
不变，那么释放速率应该会受到影响吧？ |
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D****r
发帖数: 102 | 19 【 以下文字转载自 Biology 讨论区 】
发信人: Doctor (PhD), 信区: Biology
标 题: Protein design
发信站: BBS 未名空间站 (Sun Jan 11 22:20:00 2015, 美东)
大家觉得protein design 这个方向怎样?
大牛有david tirrell, david baker, david Kaplan. |
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m******i
发帖数: 834 | 20 Nature, through the trial and error of evolution, has discovered a vast
diversity of life from what can only presumed to have been a primordial pool
of building blocks. Inspired by this success, a new Biodesign Institute
research team, led by John Chaput, is now trying to mimic the process of
Darwinian evolution in the laboratory by evolving new proteins from scratch.
Using new tricks of molecular biology, Chaput and co-workers have evolved
several new proteins in a fraction of the 3 billion yea |
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m******i
发帖数: 834 | 21 Here’s a pollution-control tip from nature: Deep inside a flooded mine in
Wisconsin, scientists from several institutions including the U.S.
Department of Energy’s Lawrence Berkeley National Laboratory have
discovered a world in which bacteria emit proteins that sweep up metal
nanoparticles into immobile clumps. Their finding may lead to innovative
ways to remediate subsurface metal toxins.
The research, which appears in the June 15, 2007 issue of the journal
Science, reveals that the proteins t |
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c*********o
发帖数: 1734 | 22 Contact me through mitbbs email if you are interested. The location is
South Carlifornia.
REQUIREMENTS:
Candidate must be a highly motivated, independent individual with strong
technical knowledge of protein stability, aggregation, chemical degradation
and molecular characterization. Additional practical experience in protein/
peptide formulation development is a plus.
Candidate should possess strong interpersonal and communication skills, and
should be able to communicate technical concepts an |
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r**********i
发帖数: 7 | 23 Thank you very much for your information. I am a Ph.D majoring in protein
preformulation and formulation. I have industrial experience in developing
liquid parenteral and lyophilized Insulin and Interferon formulations in
pharmaceutical industry, as well as in structure and stability
characterization of protein formulations using biophysical and analytical
techniques to understand degradation pathways and develop stabilization
approaches. Those requirements that seem to be of most importance to |
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r**********i
发帖数: 7 | 24 Thank you very much for your information. I am a Ph.D majoring in protein
preformulation and formulation. I have industrial experience in developing
liquid parenteral and lyophilized Insulin and Interferon formulations in
pharmaceutical industry, as well as in structure and stability
characterization of protein formulations using biophysical and analytical
techniques to understand degradation pathways and develop stabilization
approaches. Those requirements that seem to be of most importance to |
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s*******e
发帖数: 15 | 25 【 以下文字转载自 Biology 讨论区 】
发信人: stillhope (u i ), 信区: Biology
标 题: 面试protein biochemist一般怎样准备呀？ (转载)
发信站: BBS 未名空间站 (Thu Jan 3 11:41:45 2008)
发信人: stillhope (u i ), 信区: JobHunting
标 题: 面试protein biochemist一般怎样准备呀？
发信站: BBS 未名空间站 (Thu Jan 3 11:41:04 2008)
主要要求会用FPLC纯化蛋白。用是用过，只是不知道Industry面试时一般要考核哪些方
面。请有经验的人指点。谢谢。 |
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O***h
发帖数: 14 | 26 【 以下文字转载自 Biology 讨论区 】
发信人: OhYeh (comeon), 信区: Biology
标 题: Protein molecular modeling package
发信站: BBS 未名空间站 (Tue Mar 24 13:58:26 2009)
Anyone please recommend some low-cost (under $10 K) protein modeling package
?
Thanks a lot |
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l***d
发帖数: 1828 | 28 【 以下文字转载自 Biology 讨论区 】
发信人: lrpwd (lrpwd), 信区: Biology
标 题: protein discovery需要哪些Statistics skills ?
发信站: BBS 未名空间站 (Thu Nov 28 13:13:21 2013, 美东)
在做新protein的discovery,打算学一些基本统计，请问大家一般用到哪些Statistics
skills?谢谢。 |
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w*******y
发帖数: 60932 | 30 I passed by Costco (Gateway Dr.) in San Diego and as I was at the Checkout,
I saw a decent deal on 6 lbs. of 100% Whey Protein Powder by Muscle Milk in
one of those sealable packages. I believe it has 27 grams of protein and
they were all chocolate.
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w*******y
发帖数: 60932 | 31 LINK:
http://www.amazon.com/Optimum-Nutrition-Standard-Instantized-Chocolate/dp/B000GIQT2O/
Must use Subscribe & Save to get this price.
Product Features
Flavor: Double Rich Chocolate | Size: 10 lbs
One 10-pound bag of chocolate-flavored instantized whey protein
Packed with whey protein isolates
For maintaining and increasing lean muscle mass
Instantized to mix easily with a spoon
Each serving provides over 5 grams of BCAAs
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w*******y
发帖数: 60932 | 33 BSN Syntha-6 Ultra-Premium Sustained Release Protein Powder, Vanilla Ice
Cream, 2.91-Pound Jar
$18.61
Free shipping with Subscribe and Save. Just cancel once you get your order.
Link:
http://www.amazon.com/dp/B000GP5HJI/ref=pe_88830_16457170_pe_epc_d1
Great tasting protein, blend of casein/whey, perfect for me post workout or
just between meals.
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w*******y
发帖数: 60932 | 35 Purchase a 2lb tub of Nutrex Muscle Infusion lean mass protein for $29.95
and get a 5lb tub ($59.95 value) FREE, just pay $7.50 for shipping.
You can purchase any of the supplements in their store and get the 5lb tub
free, I just figured ^ is the best deal.
LINK for promo:
http://underground.nutrex.com/promo/
Add anything from these two pages:
http://underground.nutrex.com/shop/
and then type the following code after checkout for free 5lb tub of your
choice:
* For Chocolate Monster:
* Use ... 阅读全帖 |
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w*******y
发帖数: 60932 | 38 Maybe this will interest someone. I don't care for pre-workout NO
supplements because I don't like the side effects, but for $40 -- which is
what this protein usually goes for alone -- this is a pretty nice deal for
someone.
From website:
VERY LIMITED SUPPLIES: Receive a FREE SuperPump Max (160g) with Every
Purchase of MyoFusion!
**This AMAZING OFFER IS FOR A LIMITED TIME ONLY, ONLY WHILE SUPPLIES LAST!**
**This Offer is Only Valid With The Purhcase of This BOGO & Only Available
in These 3 Sele... 阅读全帖 |
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w*******y
发帖数: 60932 | 41 First do the $25 off $50 Facebook "like" link deal ( for that)
http:// />
then take advantage of 40% off Twinlab. You can find some good things to
mix and match if you dont need 10 LB of protein but this is a great discount
on a discount for high quality whey supplement.
$11.99 for 2LBs Whey Protein Fuel add 5 to cart to get over $50 (two flavors
to choose)
Link:
http://www.vitaminemporium.com/products/prod.asp?pid=146359
Example:
Subtotal: $ 59.95
Standard Shipping: $ 4.99
Estima... 阅读全帖 |
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w*******y
发帖数: 60932 | 42 Currently on sale for $36.99 each (not much of a sale), however, if you use
promo code BMSM you'll get 20% over $100. So along with their free shipping
promotion, it actually turns into a pretty nice deal ($88.78 for three tubs)
. This code can be used on anything in the store but I really needed protein
and from other posts I've read, I'm not alone in trying to find good
protein under $30 these days for a 5lb. Happy lifting!
Link:
http://www.gnc.com/product/index.jsp?productId=3557104
If someon... 阅读全帖 |
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w*******y
发帖数: 60932 | 43 Amazon has Optimum Nutrition 2:1:1 Recovery Formula in Flavor Rocky Road on
sale for $40.45 after Subscribe and Save. Like mentioned in countless
threads before, this subscription can be canceled at any time.
This same protein is $38.99 from BodyBuilding . com for only 1 3.73lb tub,
so this is a pretty hot deal.
Amazon:
http://www.amazon.com/Optimum-Nutrition-Recovery-Vanilla-Pound/
Personally, I use this stuff only on lifting days right after workouts and
have liked it quite a bit. I know peopl... 阅读全帖 |
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w*******y
发帖数: 60932 | 45 thinkThin Protein Bars in various flavors (10-pack) for $10.14, free
shipping from Amazon
Use Subscribe and Save and promo Code THINKMAYthinkThin Creamy Peanut Butter
, 2.1-Ounce Bar $10.14
thinkThin Dark Chocolate, 2.1-Ounce Bar $10.14
thinkThin Protein Bars, 2.1-Ounce Bars $10.14
thinkThin Chocolate Mudslide, 2.1-Ounce Bar $10.14
thinkThin Chocolate Covered Strawberries, 1.76-Ounce Bars $10.14
thinkThin Tangerine Cremesicle, 1.76-Ounce Units $10.14
thinkThin Chunky Peanut Butter, 2.1-Ounce Bar... 阅读全帖 |
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w*******y
发帖数: 60932 | 48 Twinlab Chocolate Surge Whey Protein 5lbs for $25 with Subscribe and Save
from Amazon
Link:
http://www.amazon.com/Twinlab-Protein-Chocolate-Surge-Pound/dp/
$28.85 One time delivery - 15% with Subscribe and Save = $24.52
After your order has been shipped you can cancel the subscription at anytime.
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