n********k
发帖数: 2818 | 1 well, as I predict 4-6ys ago on this board, I think this is the best way it
could have happened; and it did happen: Sir John Gurdon does deserve a
share...for experts who know the colorful/rich and long history of cellular/
nulear reprogramming. In a way it is not fair for Yamanaka to enjoy it alone
as there is a long history of cellular reprogramming before iPS...I
specially wrote on this long time ago, and both cell chief editor and John
Graf have written beautifully on the topic about the hi... 阅读全帖 |
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l*******i
发帖数: 153 | 2 VC可以提高MEF的reprogramming的效率，不是因为其抗氧化性，而是其histone
modification的能力，因为1）VC处理的MEF与未处理的MEF相比，其ROS水平并没下降；
2）其他的ROS scavenger并不能提高reprogramming的效率。
人的ESC和小鼠的有本质的不同，这可能是VC对人的fibroblast reprogramming没有效
果的原因。
，维
reprogr
repr |
|
l**********1
发帖数: 5204 | 3 >ESC是一个动态变化的cell
population（或者说其heterogeneity非常大），即使subcloning以后，一个cell pool
里，各个细胞之间差别可能比我们想象的要大———无论是transcriptome水平，还是
epigenetic水平——但这一点，估计得靠singel cell analysis来验证的。
Sure
plus Monte Carlo Markov Chain Stochastic approch
pls refer
Hanna J et al. (2009)
Direct cell reprogramming is a stochastic process amenable to acceleration.
Nature. 462: 595-601.
link:
http://www.ncbi.nlm.nih.gov/pubmed/19898493
or
Max Flöttmann et al. (2012)
A Stochastic Model of Epigenetic Dynamics in Somatic Cell R... 阅读全帖 |
|
u**********d
发帖数: 573 | 4 我觉得细胞周期和reprogramming的关系有意思的原因如下：
还没有报道说不能分裂的细胞也可以被“iPS”的；
去除细胞周期抑制因子（好像有p53和p16）显著提高iPS效率；
随着诱导时间的延长，iPS诱导成功的比例逐渐增加。
细胞周期中chromatin会凝缩-解聚不断反复变化，对于大多数分裂间期才表达的基因来
说，也是一个关闭开放的周期，每一次分裂就是一个表达谱重设的窗口。但是
reprogramming一定需要周期吗？周期到底如何帮助reprogramming的？
既然这么多的做表观的人都用干细胞做模型，而表观研究的方法在做干细胞的人中也越
来越普及，可能很快这些问题就有答案了。
后面的玩笑就开大了吧。大家都在迷茫，哪里有金矿啊金矿？本来还是先得看对什么问
题感兴趣，就使劲研究它。可是现实太残酷了。 |
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i****g
发帖数: 3896 | 5 丁胜在Nature的采访中有评论，ms有点酸：
Sheng Ding, a reprogramming researcher at the Gladstone Institutes in San
Francisco, California, says that the study marks “significant progress” in
the field, but notes that chemical reprogramming is unlikely to be used
widely until the team can show that it can work for human cells, not just
mouse ones. Other strategies, including one that uses RNA, can complete
reprogramming with less risk of disturbing the genes than the original iPS-
generation method, and are alread... 阅读全帖 |
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l*******i
发帖数: 153 | 6 我一直很奇怪，邓在cell文章中的观点，应该是理所当然的事，为什么很多人想不到？
大概囿于development的过程吧。其实，未分化、分化初期、终末期，这些阶段，没有
孰高孰低的区别，它们都在相同水平的高度上。这样想的话，就不会觉得由分化细胞向
干细胞reprogramming是多么困难的一件事，reprogramming这一过程与
differentiation一样，并不是那张经典图展示的那样（胚胎干细胞在“山顶”，其他
分化的细胞处在“山腰”“山脚”下）。这样想的话，邓的“跷跷板”模型，也不完全
合适，其实应该类似“台球”台的样子，不同的口袋代表不同的胚层或细胞类型；只要
外力及其方向确定好了，球可以从任何地方跑进任何一个口袋里去。这样，就可以解释
reprogramming,differentaition和transdifferentiation的发生。 |
|
c*****y
发帖数: 35 | 7 首先我认为这个方法不太靠谱，其次也没有什么科学意义。如果是机制研究，mouse已
经实现small molecule reprogramming了，human也已早有单一OCT4实现Neural
progenitor cells reprogramming的案例。
如果是应用研究，那么现在主流的reprogramming都采用非整合方法了，质粒和mRNA都
很成熟方便，也已经都商业化了。用Retro和Lenti做出来的绝大多数iPS也被认为是质
量较差的细胞株，实用价值很低。 |
|
f******g
发帖数: 1003 | 8 Reprogramming human endothelial cells to haematopoietic cells requires
vascular induction
Nature
Generating engraftable human haematopoietic cells from autologous tissues is
a potential route to new therapies for blood diseases. However, directed
differentiation of pluripotent stem cells yields haematopoietic cells that
engraft poorly. Here, we have devised a method to phenocopy the vascular-
niche microenvironment of haemogenic cells, thereby enabling reprogramming
of human endothelial cells in... 阅读全帖 |
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L****T
发帖数: 169 | 9 啥意思？我怎么记得是说他那篇Science里面的nuclear transfer reprograming的细胞
是用常规囊胚分离的hESC图片代替的，并非真正的nuclear transfer reprogramming啊
。在2004年，用人的卵子或受精卵nuclear transfer reprogramming虽然理论上可行，
但还处于效率极低以致于没有人成功过吧。 |
|
r*s
发帖数: 2555 | 10 FDA approves first gene therapy in U.S. to treat leukemia
Last Updated Aug 30, 2017 7:37 PM EDT
WASHINGTON -- Opening a new era in cancer care, the Food and Drug
Administration on Wednesday approved the first treatment that genetically
engineers patients' own blood cells into an army of assassins to seek and
destroy childhood leukemia.
The CAR-T cell treatment developed by Novartis and the University of
Pennsylvania is the first type of gene therapy to hit the U.S. market — and
one in a powerf... 阅读全帖 |
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C****g
发帖数: 2220 | 11 真是“大道之行简也”。。。
Summary
Differentiated cells can be reprogrammed to an embryonic-like state by
transfer of nuclear contents into oocytes or by fusion with embryonic stem (
ES) cells. Little is known about factors that induce this reprogramming.
Here, we demonstrate induction of pluripotent stem cells from mouse
embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-
Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was
dispensable. These cells, which we desi... 阅读全帖 |
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a******s
发帖数: 141 | 12 说的是中国刚在上Nature发的文章，好像很牛。中国科学家能用老鼠的皮细胞重组干细
胞，并以此成功造出小老鼠。
Chinese Scientists Reprogram Cells to Create Mice
WSJ.com ^ | July 24, 2009 | Gautm Naik
Posted on Friday, July 24, 2009 7:47:06 PM
By GAUTAM NAIK
Two teams of Chinese researchers working separately have reprogrammed mature
skin cells of mice to an embryonic-like state and used the resulting cells
to create live mouse offspring.
A picture released by Nature magazine shows Xiao Xiao, or "Tiny" in Chinese,
the first baby mouse created from re |
|
M***s
发帖数: 8769 | 13 Occasional problems on this vehicle are failures of the Catalytic Converter,
Air Fuel Ratio Sensor and Knock Sensor. An additional problem is failure of
the Cylinder Head Gaskets. Problems with the Catalytic Converter and Knock
Sensor will cause the Check Engine Light to illuminate. Problems with the
Air Fuel Ratio Sensor can cause drivability issues and may also cause the
Check Engine Light to illuminate, in some cases the Engine Control Module (
ECM) may need to be reprogrammed as well. Cylind... 阅读全帖 |
|
g********b
发帖数: 8461 | 14 Reprogram?什么车需要reprogram阿?
他们换O2 sensor是给你换的哪个?你这个车应该有3-4个o2 sensor,其中他们应该换的
是pre-cat的bank 2的,不过如果不贵的话可以把2个bank的都换掉因为有一个坏了另一
个也离坏不远了.
建议你用vacuum gauge看下进气歧管真空,我怀疑你的进气歧管在bank2附近漏真空导致
bank2进气量大于电脑从MAP\MAFsensor测得的值 |
|
g********b
发帖数: 8461 | 15
我从来没听过电脑需要reprogram的（改装除外），除非厂家公布说之前的程序有bug，
而且很多时候reprogram不但没解决问题反而把问题加大了。
进气歧管漏真空的话一般都是因为某个衬垫、橡胶管老化的问题，进气歧管一般是不需
要换的。
你的是V6发动机，你从发动机前方（不是车前方，是发动机前方，就是由皮带轮那一侧
）看的话是左边3个缸右边3个缸，一般左边3个缸组成bank2，右边3个缸组成bank1（也
有厂家反着的），一般这2组bank share一套进气，包括进气歧管、MAF sensor（空气
流量计）、MAP sensor（进气歧管压力传感器），但是是2个排气歧管，每个排气歧管
上各有个O2 sensor。多数车这两组排气管会汇合为1根管子然后是三元催化，在三元催
化后面还有另一个O2 sensor来监控三元催化状态（这个一般不用管），当然也有用2个
三元催化的那样就是每个三元催化后面各有一个O2 sensor。电脑主要靠MAP和MAF
sensor来计算进气量，然后通过进气量计算需要的喷油量，控制喷油嘴的脉宽，然后用
O2 sensor信号来修正喷油。一般如果电脑发现... 阅读全帖 |
|
b********y
发帖数: 27 | 16 周六出去的时候，ABS灯和ESP灯中途亮过，但是重新启动一下又就没有问题，感觉车子
也没有什么症状，就没有太在意。一朋友要回国了，周日本来想去看看他。起了个大早
，启动车，发现没有什么问题。想着在上高速之前加点油。加完油之后，车子发抖，
EPC灯、ASR灯、ESP灯，ABS灯亮，踩油门不起作用，离家比较近，重新打火了好多次，
终于可以挪了，打开双闪，一步一步挪到家。到家了之后，再后来又试了几次，车就完
全没法动了，可以启动汽车，但是启动之后，车子抖几次就马上熄火（转速指针上升了
一下就马上变为零），油门完全失效，然后就是车上各种各样的指示灯都亮了。
不幸中的万幸，1，还没有上高速，我是新手，要是高速上突然这样，也许命就挂了，
想想还是后怕；2，还好周末的早晨，路上没车，不然都没法一步一步往家里挪。
车子是01年的Audi A6，13万迈。周日自己在家鼓捣了两次，不清楚是什么原因。但是
发现电池组和车载电脑的部位泡在水里（见图,掀开盖子红色部位下面有积水，三角形
部位下面为车载电脑），前几天一直在下雨，那部分的水正常的话是应该能够自动排出
去的，可能哪个地方的管子被堵死了，不过这部分和发... 阅读全帖 |
|
O*********h
发帖数: 140 | 17 我最近车窗电控有点小问题，几个gauge都说需要reprogram Body Control Module (
BCM)。回家后research BCM的结果显示，dealer reprogramming和reset BCM都是可行
方案。
我想问下，reset BCM的好处是什么？能不能保证所有的设置都会到出厂设定？
另一方面，这么做有什么坏处／隐患？
最后问下如果reset，需要disconnect多久？1小时够用吗？
谢谢先！ |
|
a**e
发帖数: 1072 | 18 key没电了，换了一个新纽扣电池（原来的是2025），我手上只有2032（32比25厚0.7mm
，电压一样的）换上以后怎么按都没反应，不会要reprogram吧？reprogram很贵的，我
这个车dealer至少要60，我这个车比较新，好像还没有自己搞的攻略，就是外面搞好像
开价也不低。还是说找一个2025再试试？ |
|
o***m
发帖数: 203 | 19
CUSTOMER SATISFACTION NOTIFICATION M38
REPROGRAM POWERTRAIN CONTROL MODULE
Dear: (Name)
At Chrysler Group LLC, you can be assured that we are changing the way we
look at quality. To prove
our commitment to quality, the company is investing in and prioritizing
improvements for every vehicle
that we build. As part of that commitment, we are also targeting existing
vehicles on the road today and
contacting our customers to provide these quality improvements, at no charge
, that will help to imp... 阅读全帖 |
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r***r
发帖数: 414 | 20 TPMS light means the TPMS system is not working properly. It doesnt mean
the tire pressure is low.
Check the sensors, not tire pressure. is any of the sensors broken? are they
reprogrammed after you changed the left front sensor? typically it is
reprogrammed by dealers. |
|
s******y
发帖数: 613 | 21 凄惨 不幸把钥匙弄丢了 去问了 dealer 一共要~430 左右配把钥匙
121(key+cuting) +276 (the removal control box)+29 (programming fee)
另一家
350（key+ controller)+90(reprogramming fee)
ebay 上的 报价 是 $135 不过不知道怎么 cutting+reprogramming
真心请版上的车神们 帮忙推荐对策或local shop（亚特兰大地区）
谢谢 |
|
s******y
发帖数: 613 | 22 凄惨 不幸把钥匙弄丢了 去问了 dealer 一共要~430 左右配把钥匙
121(key+cuting) +276 (the removal control box)+29 (programming fee)
另一家
350（key+ controller)+90(reprogramming fee)
ebay 上的 报价 是 $135 不过不知道怎么 cutting+reprogramming
真心请版上的车神们 帮忙推荐对策或local shop（亚特兰大地区）
谢谢
FCC ID: NI4TMLF-2 |
|
s********g
发帖数: 78 | 23 The carfax of this car is a little bit weird. Is it worth buying?
Thank you
05/08/2013 8,272
Maintenance inspection completed
Computer reprogrammed
Engine/powertrain computer/module reprogrammed
One tire mounted and balanced
08/12/2013 11,201
Maintenance inspection completed
Valve cover gasket(s) replaced
Timing chain/tensioner replaced
Oil and filter changed |
|
l********e
发帖数: 848 | 24 自己 reprogram 需要两把工作的钥匙。
dealer 处好像要150-200刀。
reprogram |
|
r****a
发帖数: 1212 | 25 谢谢楼上几位的回复。
Computer reprogrammed
Engine/powertrain computer/module reprogrammed
这两个是干嘛的呢？我查这个店的其他车的Carfax记录没有这两项。 |
|
l*******s
发帖数: 7316 | 26 reprogram 可能还是有点问题吧？
前几天看旧车的时候，有辆车撞了以后，airbag没出来，但灯亮了，
然后去厂家dealer 作了 reprogram。
虽然不一定有隐患，但没事不会去reporgram的。 |
|
d*******5
发帖数: 973 | 27 到dealer service center 那里，他说如果车上不放东西的话，只要reprogram remote
和key就行了。就交130做了。
现在朋友说因为我的车是钥匙打火，就算reprogram也没用，小偷照样可以进我的车把
车开走。
请问我要不要换车门的锁，谢谢 |
|
y****p
发帖数: 87 | 28 400多"reprogramming"实在是太黑了。
其实可以买一根DCAN cable, 免费下个INPA, 装好驱动，reprogramming 也就几分钟的
事儿。
我的E90开了六年了，自己喜欢DIY，觉得这车蛮好的，计划一直开下去。
一直喜欢摄影，枪械，花了不少冤枉钱。最近开始对HIFI感兴趣，发现又是一无底洞，
呵呵 |
|
d******n
发帖数: 1768 | 29 我没有电视。车库门有两个开关：
opener，按一下就开的那种
密码锁，原房主给reprogram了交给我的，我还没时间研究怎么reprogram。
你们说的，是要reset密码锁吧，那个opener也能reset么？ |
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x**8
发帖数: 4844 | 30 这个俺当年也遇到过，希望你的也是这个问题。
俺当年的情况是两台车子怎么都连不上，墙上开关work. 查看手册说是需要有一个手持
的remote（三键的那种）才能连上车子的remote。
俺先也不懂买了一个，可是运气不好，按照说明书的做法reprogram,还是怎么都不工作
，后来才发现那个liftmaster有好几代，要看车库里那个控制盒子上一个按钮的颜色去
识别是那一代，然后买正确的手持remote,再reprogram就算是分分钟的事了。
哦，不对，你这个说是以前work过，那就是另一回事了。forget it. |
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p**m
发帖数: 3876 | 31 10 break through of 2010...
Dark Genome
The Dark Genome
Since the publication of the human genome sequence in 2001, scientists have
found that the so-called junk DNA that lies between genes actually carries
out many important functions.
Precision Cosmology
Precision Cosmology
In the past decade, cosmologists have deduced a very precise recipe for the
content of the universe, as well as instructions for putting it together,
transforming cosmology from a largely qualitative endeavor to a precision... 阅读全帖 |
|
g******d
发帖数: 511 | 32 我是这么弄的：
从ebay上买的uncutted key.到Bellevue Lexus二楼卖配件的cut.到一楼找service
department reprogramming.
我那时侯人品比较好，reprogramming没有收费，cut收了20左右，最终花费小于60.
直接找Dealer配lexus key的话，可能需要$250左右. |
|
g*****9
发帖数: 4125 | 33 You can have your volkswagen throttle mapping and shift point reprogramed.
Some newer car will learn your driving style over time, but if you are not
happy with the setting, you can reprogramed.
Say if you step on the gas padle for 1 inch it is currently set to open up
30% of the throttle, you can change that to 70% of the throttle, it will
give you a feel your car is much quicker now.
Also you can move up the shift point to a higher rpm, or hold the each gear
longer. Say moving shift point from... 阅读全帖 |
|
d**********u
发帖数: 4124 | 34 ☆─────────────────────────────────────☆
CaMKII (不作postdog) 于 (Sat Nov 8 01:18:35 2008) 提到:
发信人: CaMKII (不作postdog), 信区: Biology
标 题: 北大邓宏魁发了一篇Cell Stem Cell
发信站: BBS 未名空间站 (Fri Nov 7 22:09:29 2008)
做的也是iPSC，看来和国外跟得很紧啊。
北大今年ms很牛，除了程和平之前发的一篇Cell，程还有一篇Nature被接受，另外尚永
丰也有一篇Cell被接受。
Cell Stem Cell, Volume 3, Issue 5, 475-479, 6 November 2008
doi:10.1016/j.stem.2008.10.002
Two Supporting Factors Greatly Improve the Efficiency of Human iPSC
Generation
Yang Zhao1, 4, Xiaolei Yin1, 2, 4, Han Qin1,... 阅读全帖 |
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a****d
发帖数: 1919 | 35 最新一期的Nature paper，reprogramming from fibroblasts to functional neurons
. 一样的套路，不过还是感觉很crazy！
Direct conversion of fibroblasts to functional neurons by defined factors
Thomas Vierbuchen1,2, Austin Ostermeier1,2, Zhiping P. Pang3, Yuko Kokubu1,
Thomas C. Südhof3,4 & Marius Wernig1,2
Abstract
Cellular differentiation and lineage commitment are considered to be robust
and irreversible processes during development. Recent work has shown that
mouse and human fibroblasts can be reprogrammed to a plurip |
|
r******c
发帖数: 98 | 36 本人对stem cell有些兴趣，在此也问几个比较菜的问题
为什么不把fibroblast直接reprogram成neural stem cells呢？neural stem cell 可
以扩增，而且有paper都显示transplant neural stem cells 有一定的改善疾病症状作
用。
还有一个我对iPSCs的疑问，现在好像比较成功的方法还是使用lentivirus来reprogram
(其他的方法有报道，但efficiency很低，没有被各实验室广泛使用)。既然
lentivirus 会随机插入基因组，这样会不会导致产生出来的iPSC cell line各不相同
？说不定有些可以变成neural stem cell, 有些或许分化效率极低或者就压根不能。如
果是这样，那么用这些iPS cell line去model disease 会不会得到一些误导的结论呢
？ |
|
j********r
发帖数: 156 | 37 Is it already overheated, and not worth trying to get into?
The potential experiment might be to test if non-transcription factor
factors, such as protein kinases can
facilitate reprogramming. If true, this might facilitate the use of small
molecule for induction of iPS, and help
understand the mechanism of reprogramming. (or anyone has already working on
a similar project?)
Thanks, |
|
h******y
发帖数: 1374 | 38 Scientists turn human skin into blood
Scientists at McMaster University have discovered how to make human blood
from adult human skin.
The discovery could mean that people needing blood for surgery, cancer
treatment or treatment of blood conditions like anemia will be able to have
blood created from a patch of their own skin to provide transfusions.
Clinical trials could begin as soon as 2012.
The researchers have also shown that the conversion is direct. Making blood
from skin does not require ... 阅读全帖 |
|
R******d
发帖数: 1436 | 39 我看了一下文献，我想问的其实是：epigenetic markers reprogramming，不是
epigenetic
memory。
epigenetic memory大概指的是复制的时候epigenetic信息传递，reprogramming或许就
是我想
问的平行的变化。
我说了我不是搞这个的，所以有什么基本概念不对烦请指出来。 |
|
j********8
发帖数: 21 | 40 讲的就是基因组印记？
要是这样岂不是将灌水发挥到了极至？
Paternally Induced Transgenerational
Environmental Reprogramming of
Metabolic Gene Expression inMammals
Epigenetic information can be inherited through the
mammalian germline and represents a plausible
transgenerational carrier of environmental information.
To test whether transgenerational inheritance
of environmental information occurs in mammals, we
carried out an expression profiling screen for genes
in mice that responded to paternal diet. Offspring
of males fed... 阅读全帖 |
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t**u
发帖数: 7 | 41 帮朋友贴一个---
Postdoctoral position for Microcell Mediated Chromosome Transfer in Sloan
Kettering Institution, New York, NY.Department of Cancer Biology and
Genetics Center for Cell Engineering Sloan Kettering Institute, New York
Affiliated with Weill Medical College of Cornell University
Looking for individual who has a prior research experience on microcell
mediated chromosome transfer (MMCT).
The positions will focus on mechanistic approaches to understand 1)
reprogramming of somatic cells to plu... 阅读全帖 |
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l*******i
发帖数: 153 | 43 iPS本身最大的创新可能是1）技术的简易化，使得其迅速普及；2）其本身机制的研究
促进了epigenetics等领域的发展。
reprogramming这个概念早就存在。就体细胞向ESC逆编程而言，SCNT应该比iPS更好，
可惜的是，到目前为止还无法在人的细胞上完全实现。
iPS细胞刚出来时，几乎所有人都对其临床应用充满了期待，随着研究的深入，该领域
内怀疑其clinical therapy价值的人渐渐增多，其主要弱点有：
1）iPS与ESC的identity：Yamanaka现在似乎仍然坚持iPS与ESC是非常相似的，但是他
本人也承认现在iPS还根本无法取代ESC;相反的一派认为iPS与ESC根本就是两种不同的
细胞，无论是在transcriptome水平，还是epigenetic水平——蛋白组水平的研究，我
只看到两篇文章，不知为啥这方面的研究到目前还少之又少。当然，就目前已有的ESC
株，不同实验室分离的，不同人种来源的，相互之间也有比较大的差别，甚至这种差别
影响到了其pluripotency/分化能力。我个人的看法是，ESC是一个动态变化的cell
population（... 阅读全帖 |
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a********k
发帖数: 2273 | 44 cell封面文章。犹他HHMI Cairns和中科院刘江的背靠背文章，斑马鱼精子甲基化可以
reprogram卵子基因组。
Reprogramming the Maternal Zebrafish Genome after Fertilization to Match
the Paternal Methylation Pattern p759
Magdalena E. Potok, David A. Nix, Timothy J. Parnell, Bradley R. Cairns
Sperm, but Not Oocyte, DNA Methylome Is Inherited by Zebrafish Early Embryos
Lan Jiang, Jing Zhang, Jing-Jing Wang, Lu Wang, Li Zhang, Guoqiang Li,
Xiaodan
Yang, Xin Ma, Xin Sun, Jun Cai, Jun Zhang, Xingxu Huang, Miao Yu, Xuegeng
Wang, Feng Liu, Chung-I W... 阅读全帖 |
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h******y
发帖数: 351 | 45 终于克隆人了。
Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer
Cell, 15 May 2013
doi:10.1016/j.cell.2013.05.006
Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by
somatic cell nuclear transfer (SCNT) has been envisioned as an approach for
generating patient-matched nuclear transfer (NT)-ESCs for studies of disease
mechanisms and for developing specific therapies. Past attempts to produce
human NT-ESCs have failed secondary to early embryonic arrest of SCNT e... 阅读全帖 |
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l*******i
发帖数: 153 | 46 干细胞只是众多细胞形态中的一种，并不比其他状态（如成体干细胞，終末分化细胞等
）的细胞处在高一级的状态上，还是我之前说的那样，所有形态/状态的细胞处在同一
水平面上，理论上，所有细胞形态中，任何其中两个都可以进行相互“转化”。
当然，邓的这篇文章比yamanaka的iPS还有一系列transdifferentiation的文章，更进
一步佐证了这种理论，尽管从其实验结果推出其模型，过程似乎并不solid。从这个层
面上讲，邓的“跷跷板”模型是有局限的--不是两者之间的平衡，而应该是“多态平衡
”。
众所周知，GATA3/6, GMNN，HNF4a等TF在Blastocyst之前阶段是高表达的，其对
totipotency维持有重要作用。因此，有一种可能--正如同期preview评论的--需要考虑
到，等所选用的那些lineage specifier可能通过别的pathway，间接地促进endogenous
pluripotency genes(OCT4, NANOG， SOX2等)的表达，来实现reprogramming--类比
miRNA在reprogramming中的作用。如果这种... 阅读全帖 |
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E**********t
发帖数: 56 | 47 Xuebiao Yao, Dasheng Li and Gang Pei recently wrote an essay to Nature
Review in MCB Which is entitled “In focus: molecular and cell biology
research in China”. Here is the link: http://www.nature.com/nrm/journal/v14/n9/full/nrm3638.html In this article, they discussed the past, present and future of molecular and cell biology research in China. One part which interested me is the key MCB discoveries in China. Although most of the work are nice and likely solid work, few of them are breakthrough... 阅读全帖 |
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j******i
发帖数: 939 | 48 ipcs的挑战效率倒是其次，安全的reprogram方法才是以后拿来应用的的关键。病毒
reprogram的基本是不能拿来治疗的。 |
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z*t
发帖数: 863 | 49 important tip in cell selection
Primary cells should be used. We have found that it is difficult to
reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro
, while fresh MEF are competent.
Cells from mice older than one week showed very poor reprogramming
efficiency under the current protocol.
hmmm...... |
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z*t
发帖数: 863 | 50 important tip in cell selection
Primary cells should be used. We have found that it is difficult to
reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro
, while fresh MEF are competent.
Cells from mice older than one week showed very poor reprogramming
efficiency under the current protocol.
hmmm...... |
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