g*****3
发帖数: 107 | 1 最近在提纯一个GST-tag的蛋白，蛋白表达在pellet需要denature condition提纯， 纯
化后需要做活性检测。怎样才能让蛋白的活性在提纯过程中不丢失呢，需要用
refolding kit吗？谢谢 | a***e
发帖数: 1010 | 2 yeah, you need refolding most of time.
An alternative method is adding some sarkosyl during purification, which
solublizes your protein into the supernatant. |
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