b******r 发帖数: 111 | 1 I need PCR 5-flanking of mouse APOE genomic DNA(9kb upstrean of exon 1),and
human APOE3,4 genomic DNA(4kb). I have tested 10 primer pairs and their
combinations.But till now,can not get the target PCR product.Maximum is non-
specific 2-4 kb Anyone knows any vectors and their vendors contain the
mouse and human APOE full length genomic DNA ? | X***n 发帖数: 366 | 2 genome.ucsc.edu
open the BAC track. Go to gene ApoE. Select the BAC clone. You can order
from Invitrogen.
and
non-
【在 b******r 的大作中提到】![](/moin_static193/solenoid/img/up.png) : I need PCR 5-flanking of mouse APOE genomic DNA(9kb upstrean of exon 1),and : human APOE3,4 genomic DNA(4kb). I have tested 10 primer pairs and their : combinations.But till now,can not get the target PCR product.Maximum is non- : specific 2-4 kb Anyone knows any vectors and their vendors contain the : mouse and human APOE full length genomic DNA ?
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