m******t 发帖数: 109 | 1 i pulled down chromatin with anti-acetyl H3k9 antibody and normal IgG as
control. then i did q-PCR to pick up a DNA segment in a promoter. i found,
compared with normal IgG, the specific antibody enriched hundreds -over 1
thousand times of target DNA segment. does it make sense? |
k*****n 发帖数: 323 | 2 where is your negative control? |
p******g 发帖数: 180 | 3 一般是用anti-H3,anti-acetyl H3k9 一起做,anti-H3作为control。 |
a**********s 发帖数: 8 | 4 和我前一段时间做的结果接近。IgG大约为Input的0.01%,Acetyl H3k9为10% |