y****6
发帖数: 196 | 1 Does anyone know why Trizon reagent can separate DNA from RNA?
Thanks | K**R
发帖数: 193 | 2 definitely yes
meddle layer including DNA | n******e
发帖数: 110 | | c******r
发帖数: 3778 | 4 trizol的意思就是可以分离dna，rna，和protein。具体步骤看说明书
【在 y****6 的大作中提到】
: Does anyone know why Trizon reagent can separate DNA from RNA?
: Thanks
| D**********e
发帖数: 41 | 5 My understanding is in acidic pH, because RNA is single stranded, the bases
of RNA ionize with H+, and is soluble in aqueous phase. While DNA is double
stranded, it's base is not exposed, it's not soluble in either aqueous or
organic phase. In basic pH, the phosphate backbone of DNA form salt with Na+
, and become soluble in aqueous phase. | y****6
发帖数: 196 | 6 This sounds reasonable to me. Thanks.
bases
double
Na+
【在 D**********e 的大作中提到】
: My understanding is in acidic pH, because RNA is single stranded, the bases
: of RNA ionize with H+, and is soluble in aqueous phase. While DNA is double
: stranded, it's base is not exposed, it's not soluble in either aqueous or
: organic phase. In basic pH, the phosphate backbone of DNA form salt with Na+
: , and become soluble in aqueous phase.
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