k***x
发帖数: 34 | 1 看了Nature上那篇Inuk的原始人genome的paper，整个genome sequence出来之后，也只找到几个特征性的SNP，比如眼睛的颜色，earwax，门牙什么的。
是不是可以找很多种特征基因，只是这篇paper没专注这方面？还是特征性SNP就是不好找？
读了一下full genome sequencing的wiki，现在有这么多家公司都在开发，而且眼看成本就要到$1000以下了。 即使不提sequence出来的depth和false positive rate，就是质量极高的sequence，如果不知道怎么解释data，还是没有什么意义吧。有没有朋友了解的给介绍一下，谢谢了。 |
|
M***7
发帖数: 2420 | 2 Hi there.
I was trying to collect some HIV whole genome sequences as many as possible.
The only thing I found is this HIV database http://www.hiv.lanl.gov/content/index. However, I did not get any useful HIV whole genome sequence from NCBI.
Dose anyone know is there any other public HIV whole genome sequences
available?
Thanks |
|
b******r
发帖数: 111 | 3 I need PCR 5-flanking of mouse APOE genomic DNA(9kb upstrean of exon 1),and
human APOE3,4 genomic DNA(4kb). I have tested 10 primer pairs and their
combinations.But till now,can not get the target PCR product.Maximum is non-
specific 2-4 kb Anyone knows any vectors and their vendors contain the
mouse and human APOE full length genomic DNA ? |
|
a*****g
发帖数: 543 | 4 Just my two cents:
1. 我不反对也不支持CSC的model.但是光从mutation的普遍性来说并不能否定CSC. 我
们现在还没法确定这些genetic heterogeneity是driver 还是passager.大多数只是ca
ncer的result而不是cause。Moreover, it is possible that CSC and Non-CSCs all
have genetic changes;remember the so-called CSCs don't have to follow the
biology of typical stem cells.
If cancer strictly follows evolution model, should one imagine certain gene
tic lesions gain dominance?
As for the genomic stability, why should we believe it is promoting tumor?
As far as I know,... 阅读全帖 |
|
b****n
发帖数: 110 | 5 Marie Curie POST-DOC fellowship application
University of Barcelona, Department of Genetics
Our new group on the field of Functional Evo-Devo and Genomics on Chordates
is looking for candidates to apply for the forthcoming FP7 POST-DOC
FELLOWSHIP from the Marie Curie program (Opening call March 13)
Candidates have to be enthusiastic, and need to have a competitive CV to
successfully apply for the fellowship. Experience in Molecular Genetics,
Transgenesis and Developmental Biology, and (or) Backg... 阅读全帖 |
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w****i
发帖数: 23 | 6 What is the future of the next-generation sequencing? More importantly, what
kind of jobs can it
create for Biologist? and how many?
http://www.fiercebiotech.com/story/buzz-genomics-startup-halcyo
quote:
"Many groups rushed into the next-generation sequencing game over the past
decade, so it's unsurprising to see news about some thinning of the genomics
herd. Halcyon Molecular has shut down after other groups leapt ahead in
bringing to market new technologies for decoding DNA quickly and
inexpen... 阅读全帖 |
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s*****0
发帖数: 357 | 7 A few comments on the NGS impact in the future:
1. The sequencing cost is dramatically decreasing, outpacing the Moore's law
prediction. Exome sequencing as part of patient profile will take off very
soon, and whole genome sequencing will not be far away. The cross-referenced
data will be highly valuable to healthcare industry players (insurance,
pharma, EMRs, etc), and in the long run the cost of data will be much higher
than that of sequencing.
2. So far the market is highly fragmented, with a... 阅读全帖 |
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b******r
发帖数: 111 | 8 These days, almost make me desperate to death. As a 2-year post-doc,who did
lots of cloning, I fail to do such an easy work. Isolate mouse genomic DNA
by phenol/chloroform,75%ethanol precipitation. Then digest by HindIII
restriction enzyme as follows:
genomic DNA: 2 ug,1ug,and 0.4ug;
HindIII enzyme: 70 units(3.5ul,and different units);
Buffer: 5 ul
add water to 50ul
37 degree for 10 hours. Run gel.Genomic DNA is still a strong band, not
smear at all. I have try HindIII from NEB and Roche com... 阅读全帖 |
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x*******6
发帖数: 6 | 9 小弟最近在申请博后，博士在一个偏理论组做的是comparative genomics的数据分析，
主要是细菌的，博士组里的强项是phylogenetic network，个人的生信背景偏
phylogenetics和pathway modeling多些。最近看着好多在sequencing center做NGS的
同学发了一水的牛paper，很心动。想博后往sequencing上靠靠不知有否可能，比如
assembly，resequencing，structural variation或者Chip-seq, RNA-seq之类的数据
分析。现在对这方面了解甚少。
个人觉得comparative genomics还是有很多可做，但是至今个人的paper档次很低，都
是bmc之类。而且comparative genomics做多了，觉得不是很有motivation，NGS感觉可
是说出不少临床的应用来，觉得工作更有价值些，呵呵。
一直在单枪匹马干（老板是放羊型），觉得特别吃力。工作不系统，打一枪换个地方。
老板也推荐了几个博后的组，方向都和博士比较接近，中等学校（top 10~20），4，5... 阅读全帖 |
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l*********1
发帖数: 351 | 10 外行小白请教下：
RNA-programmed genome editing in human cells
RNA-Guided Human Genome Engineering via Cas9
Multiplex Genome Engineering Using CRISPR/Cas Systems
“It’s too early to declare total victory” over TALENs and zinc-fingers,
Church said, “but it looks promising.”
是不是说TALEN engineering的坑就不用跳了啊？多谢指教。 |
|
G*****h
发帖数: 320 | 11 Design several pairs of primers and then run BLAST searches of these primers
against the host genome sequences (and closely related species) if they are
available.
Rule out these primers with high identities, particularly those with
relatively long identical sequences at 3' ends.
Make sure that 3' ends of your primers have at least one base (the more, the
better) that does not match any host genome sequences.
Test your primers using host genome DNA alone to ensure there are no non-
specific ampl... 阅读全帖 |
|
x********6
发帖数: 986 | 12 最近计划要开始做一个project是用几个deep sequencing方法来检测一种先天遗传疾病
（已被辨认是由一个控制染色体重构的单基因突变造成的）的基因组层面的缺陷（包括
甲基化，乙酰化，长距离转录等等）， 刚从其他搞临床的实验室获得了三个不同病人
（分别具有该基因的不同突变位点）的细胞样品。
这几天阅读了几篇发表在genome research和Nucleic Acids Research的几篇类似的文
章发现这些工作都测了大概几十个病人的样品。一方面由于我们实验室是一个basic
research lab，无法获得更多的人类临床样本，另外一方面目前的funding也不能支持
做太多样品的deep sequencing。
因此想请教一下各位大侠想genome research， Nucleic Acids Research， genome
biology或者Human Mutation这些期刊对于这种疾病基因组学的研究有没有相关的要求
一定要多少病人的样本才可以接受，或者用我们手上这三种细胞做就可以有足够的代表
性了？ 先谢谢大家啦！ |
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l**********1
发帖数: 5204 | 13 Pls refer one review
By Madrigal P and Krajewski P. (2012).
Current bioinformatic approaches to identify DNase I hypersensitive sites
and genomic footprints from DNase-seq data.
Front Genet. 3:230.
cited from its pp2:
>With sufficiently deepsequencing,the
>so-called“digital genomic footprinting”
>technique can reveal single protein-
>binding events(Hesselberth et al.,2009).
>Unlike ChIP-seq,which is specific for the
>protein under study,footprints identify
>narrow DNA regions that can be bound
>... 阅读全帖 |
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n*******l
发帖数: 190 | 14 我一直用qiagen的kit.没有发现什么genomic dna的污染。请问你是通过什么发现
genomic dna污染的？
另外，现在qiagen有一种genomic dna elumination column,不需要DNase treatment,
不知道怎么样。 |
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a**m
发帖数: 184 | 15 phd 第三年，主要做cancer genomics method development
前两年大概自己攒了$1000-$2000的travel funds
另外老板的travel funds自己用不掉，说phd第四年开始要我travel till I get sick
of it
求大牛推荐一下cancer genomics 相关的 conference/workshop
bioinformatics领域的也可以（副业搞得是chip-seq, structural variants
detection）
我现在打算要去的是：
1. AACR, Philadelphia April 2015
2. ASHG, Baltimore Oct 2015 (都花不了多少钱，学校在东北部)
3. EMBL cancer genomics, Germany November 2015 http://www.embl.de/training/events/2015/CAN15-01/index.html, 老板okay了这个会，但是具体咋样？)
4. JSM和ENAR其实是老板的领域，但是我总觉... 阅读全帖 |
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r**********e
发帖数: 587 | 16 PhD第五年了要张罗毕业考虑未来了，所以来请教下各位前途选择问题。
我研究方向以及未来的兴趣倒是蛮确定的，就是medical genomics。 我具体是在
neurology系做神经疾病的基因组学，生物信息；简单来说就是寻找neurological
disorder的致病相关的genetic variants；以及找到的variants的深层的分子机制。
所以我具体的training，一方面是bioinformatics相关的计算机的东西以及human
genetics/genome science; 另外一方面则是由此引申出的epigenetics, splicing等等
基因调控的基础生物的东西，所以也做了很多传统的实验。当然了，老板是MD，实验室
主题是neurology，所以自然也了解一些神经相关的东西。
所以我的优势是方向比较热，而且我涉及的范围挺广的。但优势也是劣势，那就是我不
精通某个小方向，比如，相对于专业的bioinformatician我算半路出家远不如他们，当
然对于一般zero 计算经验的同学我还是要强的多，至少有很多分析NGS数据的经验。还
比如，我做实验的... 阅读全帖 |
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m*********D
发帖数: 1727 | 17 他science文章的protocol里是puromycin七天，然后是drug treatment七天或十四天。
要整合的话（stable cell line)，时间是该差不多了。
你说的先作Cas9的stable我要考虑一下。就是作stable cell line太花时间。Cas9的表
达silence问题该不大。一般stable在我手里是三个月之后才出现基因表达走下坡。
Lentivirus感染效率高的话，一个vector省很多事啊。上次作点突变，我用普通的
lipofectamine 3000作transfection, 效率太差，少于10%的细胞能在三天的puromycin
selection后活下来。也许我用的puromycin量太高，2.5ug/ml。当时作了一个三天的
killing，找到能三天杀完host cell的最低量，就用上了。
再向你请教一下：vector整合进genome后，拿到genomic DNA，再用primers来扩增整合
的guide Sequences。这对PCR primers 就是直接从vector来的吗？我想象这对primers
正好在gu... 阅读全帖 |
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m*********D
发帖数: 1727 | 18 你看我昨天来问的时候就是这个上面有些糊涂。我现在也还担心：如果CRISPR
function很快，virus进细胞后表达gRNA/Cas9，马上ko了一个pathway上的基因，3天的
puromycin selection期间，没整合进genome,这个细胞也可能survive的。所以，我以
为sequencing整个genome来找呢。
既然是比较两个pool,遗漏一些或false positive是难免的。估计搞的几个就好。现在
的signal transduction的“网络”已经很成熟了，找到几个估计就会有谱了。看了
Feng zhang的method section,细胞用量很大，估计是不是考虑了整合进genome的效率
不是很高这个因素。
我这个因是新的pathway,自己有potency到了drug级别的small molecule inhibitor，
所以，就想自己试试。
谢谢！
gRNA |
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w********2
发帖数: 632 | 19 3D genome seems to focus on the spacial structure of the genome. It is not
easy, because spacial structure can change during sampling and extraction.
Even totally in natural form, dynamics happens because the genome is too big
too long. Once into the structural biology field, advanced instruments are
needed, EM, X-Ray, NMR, FT-MS, etc. Good research field, but high risk and
long term. |
|
n********b
发帖数: 27 | 20 There is a genomics scientist/research specialist position opening at the
Center for Genomics at LLU, Loma Linda, CA. Please refer if you know anyone
that is looking for a genomics position (see link for the job description).
Please have the CV send to Dr. Wang ([email protected]/* */). Thanks!
https://www.healthcaresource.com/lomalinda/index.cfm?fuseaction=search.
jobDetails&template=dsp_job_details.cfm&cJobId=111748 |
|
x****e
发帖数: 55 | 21 PhD Student and Postdoc Positions in Genomics, Bioinformatics
Center for Human Identification
University of North Texas Health Science Center
Fort Worth, TX, USA
The University of North Texas Center for Human Identification (UNTCHI) is an
accredited forensic laboratory which is globally recognized as a leader in
forensic genetics. With funding support from the State of Texas and various
Federal Government agencies, UNTCHI is a resource for international and
national legal systems, provides foren... 阅读全帖 |
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b**d
发帖数: 18 | 22 【 以下文字转载自 Biology 讨论区 】
发信人: bald (小颓), 信区: Biology
标 题: 新泽西公司找有genomics背景的PhD Scientist
发信站: BBS 未名空间站 (Wed Nov 3 21:06:35 2010, 美东)
公司在找有genomics背景的PhD Scientist.我们是service company,做大量的SNP/
mutation analysis.希望你熟悉 how to look for genomic sequence in the
database, PCR primer design, PCR sequencing data analysis and call mutation/
insertion/deletion etc.如果你有分子生物学的背景(mutagenesis, cloning etc)会
更好。我们希望招PhD with 0-2 years postdoc experience. |
|
a****o
发帖数: 1786 | 23 >***Save the Date!***
>
>The new Program in Genetics and Genomics of BBS invites you to attend
>the Inaugural Symposium in Genetics and Genomics, featuring past and
>current graduate students of Harvard Medical School.
>
>Inaugural Genetics and Genomics Symposium Monday, December 14, 2009
>1:00pm-5:00pm, reception to follow Joseph B. Martin Conference Center,
>Rotunda Room (third floor)
>
>Speakers:
>
>Leonard Guarente, Professor of Biology, MIT "Sirtuins, Aging, and
>Disease"
>
>Rachel Dutton, |
|
a*m
发帖数: 1217 | 24 这个是不用注册就可以参加的么 看起来很好
>***Save the Date!***
>
>The new Program in Genetics and Genomics of BBS invites you to attend
>the Inaugural Symposium in Genetics and Genomics, featuring past and
>current graduate students of Harvard Medical School.
>
>Inaugural Genetics and Genomics Symposium Monday, December 14, 2009
>1:00pm-5:00pm, reception to follow Joseph B. Martin Conference Center,
>Rotunda Room (third floor)
>
>Speakers:
>
>Leonard Guarente, Professor of Biology, MIT "Sirtuins, Aging, and
>Disea |
|
a****o
发帖数: 1786 | 25 【 以下文字转载自 Biology 讨论区 】
发信人: nextgenlab (ox), 信区: Biology
标 题: Position Opening-Research Associate II-Genomics-Maricroarray Core-South CA Area
关键字: Genomics,Microarray,RA-II
发信站: BBS 未名空间站 (Sun May 2 10:58:02 2010, 美东)
There is a Research Associate II position opening in a Genomics-Microarray
Core in a Medical Center in southern CA-Los Angeles Area. Please send your
resume/CV to c*****[email protected] and/or o****[email protected], if you are interested
in it. Thanks.
See job descriptions: |
|
|
发帖数: 1 | 27 【 以下文字转载自 Computation 讨论区 】
发信人: Sp2018 (为什么我这么多不懂), 信区: Computation
标 题: Re: tools for merging assembled genome (转载)
发信站: BBS 未名空间站 (Sat Apr 21 19:18:54 2018, 美东)
发信人: Sp2018 (为什么我这么多不懂), 信区: Biology
标 题: Re: tools for merging assembled genome
发信站: BBS 未名空间站 (Sat Apr 21 19:16:42 2018, 美东)
interesting.
如果没有现成工具，应该可以叫CS 的人做出来。。 |
|
发帖数: 1 | 28 【 以下文字转载自 Biology 讨论区 】
发信人: capry (IA), 信区: Biology
标 题: RNA-seq data assembler vs genomic shot gun data assembler
发信站: BBS 未名空间站 (Sat Jan 6 09:17:47 2018, 美东)
版上有人能介绍下它们之间的区别嘛？
想用cufflinks to assemble shot-gun genome data, 不知可行否
多谢！ |
|
n********b
发帖数: 27 | 29 There is an opening of a Research Specialist-Genomics in the Center for
Genomics of a University. Please see the link for detail. Please send your
CV to [email protected]/* */ if you are interested in. Thanks!
https://www.healthcaresource.com/lomalinda/index.cfm?fuseaction=search.
jobDetails&template=dsp_job_details.cfm&cJobId=111748 |
|
m****s
发帖数: 18160 | 30 【 以下文字转载自 Faculty 讨论区 】
发信人: silent (年年), 信区: Faculty
标 题: Vacancies in BioEnergy Genomics Centre,Qingdao Institute of (转载)
发信站: BBS 未名空间站 (Thu Oct 18 06:14:38 2007)
发信人: silent (年年), 信区: Postdoc
标 题: Vacancies in BioEnergy Genomics Centre,Qingdao Institute of Bioenergy and Biomass Technology, CAS
发信站: BBS 未名空间站 (Thu Oct 18 06:13:05 2007)
中国科学院青岛生物能源与过程研究所生物能源基因组中心招聘
一、中国科学院青岛生物能源与过程研究所生物能源基因组中心简介：
中国科学院青岛生物能源与过程研究所（筹） （Qingdao Institute of Biomass
Energy and Bioprocess Technology, Chinese Aca |
|
b*p
发帖数: 242 | 31 Commercializing Full Genome Sequencing (Genome Bubble 2.0?)
While service is the future (GNOM), currently it is still about equipment R&
D (PACB), though.
Any Comments? |
|
i********f
发帖数: 206 | 32 【 以下文字转载自 Biology 讨论区 】
发信人: iamicewolf (icewolf), 信区: Biology
标 题: 求审稿机会NGS, Genomics, Bioinformatics
发信站: BBS 未名空间站 (Wed May 1 00:44:39 2013, 美东)
博士学的生物信息,主要做植物的基因组和表达的分析.有统计的硕士学位,熟悉常用的
统计分析.对建生物的数据库,以及网页工具比较熟悉.
现在工作主要是做人的exome sequencing部分,用于癌症以及遗传疾病的检测.
Keywords:
NGS
exome sequencing
genomics
bioinformatics
谢谢 |
|
i*******i
发帖数: 145 | 33 【 以下文字转载自 SanFrancisco 讨论区 】
发信人: ilikepili (ilikepili), 信区: SanFrancisco
标 题: 生物信息求内推(bioinformatics/genomics/NGS/algorithms/softw (转载)
发信站: BBS 未名空间站 (Mon Apr 29 16:18:36 2013, 美东)
发信人: ilikepili (ilikepili), 信区: JobMarket
标 题: 生物信息求内推(bioinformatics/genomics/NGS/algorithms/software/)
发信站: BBS 未名空间站 (Mon Apr 29 14:48:42 2013, 美东)
小弟我今年8月份博士计算机科学（计算生物学和生物信息方向）毕业，9月份可以上班
（OPT）。
现在最希望是找一些生物技术或者制药公司。我博士期间主要的研究方向是做算法设计
和生物信息软件开发。我自以为编程还是不错的，C, C++, Java, Perl, Python,
Matlab, R 都没有问题。另外在一个医学研究所做了... 阅读全帖 |
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S**********l
发帖数: 30 | 34 【 以下文字转载自 Biology 讨论区 】
发信人: SaddenedSoul (未收到激活码), 信区: Biology
标 题: 默克 Boston postdoc position, Bioinformatics/Genomics
发信站: BBS 未名空间站 (Fri Oct 16 17:42:34 2015, 美东)
有意者请直接和我联系， 把 CV 和 cover letter (optional) 发 给我：x i n w e i
_ s h e r @merck.com (去掉空格）
https://merck.taleo.net/careersection/merck_external_career_section/
jobdetail.ftl?job=IT+000402&lang=en
Responsibilities include but are not limited to:
• Develop computational methods and pipeline for vaccine design using
NGS data
R... 阅读全帖 |
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S**********l
发帖数: 30 | 35 几个月前在这里登过。Offer被(国)人拿去做了备胎。耽误了几个月，现在从新再来。
Responsibilities include but are not limited to:
• Develop computational methods and pipeline for vaccine design using
NGS data
• Apply the established methods on Merck data and public data and
identify vaccine candidate genes.
• Identify biomarkers associated with response to treatment and
patients stratification using population genomics approach.
Qualifications:
Ph.D. in related areas.
Demonstrated experience and knowledge on bioinfo... 阅读全帖 |
|
C******n
发帖数: 941 | 36 From Fortune Feb. 19 2001
By Brian O'Keefe
Technicians are working to find drug applications from Celera's database.
Brian O'Keefe
Last year Celera Genomics and its president, J. Craig Venter, shook up the s
cientific world by successfully sequencing the human genome faster than anyo
ne—even Venter—had predicted. But when the hype faded, investors were left
with one big question: How could Celera turn its accomplishment into financ
ial gain?
The answer wasn't immediately clear. Venter insisted t |
|
r***g
发帖数: 6 | 37 (FROM SCIENCE)
THE RICE GENOME:
Beijing Genomics Institute: From Standing Start to Sequencing Superpower
Dennis Normile*
Good timing and determination have helped geneticist Yang Huanming create an
institute that has catapulted China into the front ranks of sequencing
BEIJING AND HANGZHOU--In August 1998, geneticist Yang Huanming led a skeptic
al crowd of scientists from around the world through a new, two-story brick
building in the northern reaches of Beijing. As the scientists trooped throu
g |
|
|
s*r
发帖数: 2757 | 39 http://www.pnas.org/content/early/2010/04/28/0914771107
Comparing genomes to computer operating systems in terms of the topology and
evolution of their regulatory control networks
The genome has often been called the operating system (OS) for a living
organism. A computer OS is described by a regulatory control network termed
the call graph, which is analogous to the transcriptional regulatory network
in a cell. To apply our firsthand knowledge of the architecture of software
systems to understa |
|
K**4
发帖数: 1015 | 40 没看懂
两个不相关的东西做个对比
难道明天我比较下genome和语言结构,也能发?
后天别人比较下genome 和 天体结构?
and
termed
network
software
( |
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e****l
发帖数: 204 | 41 The biggest differences between a genome and a computer program is that a
computer program can not tolerate very small mistakes while a genome can
have mutations. Mutation drives evolution. Can we design a computer program
that
has the ability to change itself but still functional? |
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n********b
发帖数: 27 | 42 There is position opening in a Genomics Core-Staff Scientist (supported by
hard-money) in a medical center/research institute in S CA area with good
retirement/benefit package. Please send you CV to o****[email protected], if you
are interested in. Thanks.
See job descriptions:
Staff Scientist
Functional Genomics Core
1. Perform bench work related to research projects & services using
different DNA microarray technologies/platforms including Affymetrix
GeneChip, Agilent, Roche NimbleGen and Illuman’s |
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s*r
发帖数: 2757 | 44 http://www.spiegel.de/international/world/0,1518,709174,00.html
In a SPIEGEL interview, genetic scientist Craig Venter discusses the 10
years he spent sequencing the human genome, why we have learned so little
from it a decade on and the potential for mass production of artificial life
forms that could be used to produce fuels and other resources.
SPIEGEL: Mr. Venter, when the elite among gene researchers undertook the
decoding of the human genome, you were their greatest enemy. They called you |
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n******u
发帖数: 418 | 45 我直接把chromatin pellet用SDS loading buffer去boil几分钟然后用来跑胶，但是可
能是因
为里面含有genomic DNA的原因，会比较sticky，虽然跑SDS-PAGE胶的时候sample不是很
sticky，但是跑出来之后做western发现整条lane城smear状，特别是大size的protein
，有人建
议我做一下sonication把genomic DNA震碎，我做了但是貌似不起作用，还有人建议我
treat
with DNase，但是我从来没做过，不知道这个会不会起作用，这里有没有人做过类似的
实验，如果
DNase treatment有作用的话，要加多少量并且在什么条件下treat多长时间呢？
如果这个方法也不行的话，请问还有什么别的办法可以解决smear的问题？
谢谢各位了！ |
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b**d
发帖数: 18 | 46 公司在找有genomics背景的PhD Scientist.我们是service company,做大量的SNP/
mutation analysis.希望你熟悉 how to look for genomic sequence in the
database, PCR primer design, PCR sequencing data analysis and call mutation/
insertion/deletion etc.如果你有分子生物学的背景(mutagenesis, cloning etc)会
更好。我们希望招PhD with 0-2 years postdoc experience. |
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e*****t
发帖数: 642 | 48 我要的是whole cancer genome seq. 现在可以看到的好像只是snp （我不确定，在网
站上找不到）
在哪可以下你说的 cancer genome seq。我找不到。 |
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u*********1
发帖数: 2518 | 49 记得原来就发过问，但没得到很好的回答。这次综合下自己的一些想法，请教下各位前
辈。如果有什么说的不对或者naive的地方还请多多包涵。
虽然刚开始读phd，但其实已经感觉到压力很大。以后想做genomics，一方面是自己的
确很有兴趣，另外一方面也是想做点跨学科的东西，以后如果科研做不下去，去
industry survival的时候或许有点优势。
或者干脆说：我想像人家mass spec的人学一门“手艺”，以后有口饭吃，过上朝九晚
五的生活，养活自己一个人就可以了。而不是做postdoc太多的不确定的因素，每天都
在着急。
1.请问在industry或者大学做genomics，具体到底是做什么呢？因为大家都是把那些
sample送到公司，公司帮你做，那是不是说在公司操作illumina这样的仪器也算是一个
饭碗？（就好比做mass spec的人）我们这种biology phd training出来的可能胜任这
样的工作么？另外，拿到测序结果后的bioinformatics也算另外一种工作吧？
2.我问了身边很多做这方面的人，大部分人还是做bench work的。有的老板是请一个
bi... 阅读全帖 |
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e*****t
发帖数: 642 | 50 知道一个mRNA snp和它在genome上的位置。如何知道编码的蛋白突变位点？genome
browser，ensembl或者ncbi应该都行，但是看了半天没看出来。高手帮帮忙 |
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