P**A
发帖数: 1575 | 1 广义的讲，用宠物配种（breeding）的都可以叫breeder，包括backyard breeder，
puppy mill，版上自己家宠物不去kc，配种生崽了的也是，都可以说自己是breeder，
有没有哪里发的各种license都没啥关系。
通常正常人说的breeder，是指“靠谱的”breeder，上次贴过一个wiki的定义，相对于
利润优先的puppy mills，我们说的breeder，都不是以赚钱为主要目的的， is a
person who practices the vocation of mating carefully selected specimens of
the same breed to reproduce specific, consistently replicable qualities and
characteristics. 很多时候，这样的breeder都是纯兴趣为动力，赔钱做的。
如果只要付钱就可以随便卖出去随便别人繁殖不加控制的，那就只是商人罢了。
区别？ |
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a*********7
发帖数: 30080 | 2 文中提到的Forbien Archeolgy(Forbidden Archeology)一书, 可参见
http://en.wikipedia.org/wiki/Michael_Cremo#Forbidden_Archeology
"Forbidden Archeology has been criticized for failing to test simpler
hypotheses before proceeding to propose more complex ones (a violation of
Occam's razor) and for relying heavily on outdated evidence (often from the
19th and early 20th century).[15] Tom Morrow of the National Center for
Science Education noted that Cremo's "specimens no longer exist" and dubbed
the work pseudos |
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c**i
发帖数: 6973 | 3 Judith Martin, China's Cuddly Emissaries. Wall Street Journal, July 2, 2011
http://online.wsj.com/article/SB10001424052702
303339904576405982375338112.html?mod=WSJ_Books_LS_Books_6
book review on Henry Nicholls, The Way of the Panda: The curious history of
China's political animal. Profile Books, 2011)
Quote: "Pandas were formally discovered quite late, in 1869 * * * they had
never been mentioned or depicted in Chinese literature or art. * * * Then
one day a French missionary and collector of an... 阅读全帖 |
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N****f
发帖数: 25759 | 4 Zkss?
虽然罕有任何残留的specimens，不过汉代陶器中有不少模型传世，画
像砖上也有反映。俺倒是觉得秦汉时代的高阙望楼很有些风格。 |
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a***k
发帖数: 1038 | 5 I
The way led along upon what had once been the embankment of a railroad. But
no train had run upon it for many years. The forest on either side swelled
up the slopes of the embankment and crested across it in a green wave of
trees and bushes. The trail was as narrow as a man's body, and was no more
than a wild-animal runway.
Occasionally, a piece of rusty iron, showing through the forest-mold,
advertised that the rail and the ties still remained. In one place, a ten-
inch tree, bursting through... 阅读全帖 |
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m***o
发帖数: 1367 | 6 Trees- an illustrated identifier and encyclopedia
By Tony Russell and Catherine Cutler
Hermes House 2004 (256 pp)
Borders的减价书，$6
虽然搬家已经很痛苦了，还是忍不住把这本书带回家。书里这样介绍我一直向往的
菩提树：Bo Tree (Ficus religiosa)
It is said that the Buddha was sitting beneath a bo tree when he attained
enlightenment. This type of fig is incredibly long lived, with specimens
thought to be more than 2,000 years old. It is sacred to Buddhists and Hindus
and is regularly seen growing in the grounds of temples. A strangling
climbe |
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c*****t
发帖数: 1879 | 7 I was a bit confused by the ending actually.
The story goes like this:
Bruce was a prisoner sometime in the future. He always had this
bad dream that he saw a man getting shot and a lady screaming
and runing toward that man (repeated many times in the movie).
To reduce his sentence, he accepted an assignment to go above
ground to collect specimens. Since in year 20xx the only people
alive were living underground and all people living above the
ground had been killed by a deadly virus, this ass |
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C***r
发帖数: 759 | 8 CHAP. 5.--OF THE FRATRICIDAL ACT OF THE FOUNDER OF THE
EARTHLY CITY, AND THE CORRESPONDING CRIME OF THE
FOUNDER OF ROME.
Thus the founder of the earthly city was a fratricide.
Overcome with envy, he slew his own brother, a citizen of
the eternal city, and a sojourner on earth. So that
we cannot be surprised that this first specimen, or, as the
Greeks say, archetype of crime, should, long afterwards,
find a corresponding crime at the foundation
of that city which was destined to reign over so man |
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l*****a
发帖数: 38403 | 10 【 以下文字转载自 Belief 讨论区 】
发信人: crel (随风), 信区: Belief
标 题: Re: 关于C14探测地球生物年龄的问题
发信站: The unknown SPACE (Fri Jun 7 15:25:17 2002) WWW-POST
OK, about right on C-14.
I did some homework, and it is quite rewarding as I can kick someone's ass,
and hard, very hard.
In short, the three examples that you bring up with are quite representative
among creationists, who either lie on purpose, or use old method on purpose,
or choose inapproapriate sample on purpose, or both. They are famous lies.
They are so r... 阅读全帖 |
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l*****a
发帖数: 38403 | 13 Linguistics of Pentecostal glossolalia
William J. Samarin, a linguist from the University of Toronto, published a
thorough assessment of Pentecostal glossolalia that became a classic work on
its linguistic characteristics.[5] His assessment was based on a large
sample of glossolalia recorded in public and private Christian meetings in
Italy, Holland, Jamaica, Canada and the USA over the course of five years;
his wide range included the Puerto Ricans of the Bronx, the Snake Handlers
of the Appala... 阅读全帖 |
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J*****3
发帖数: 4298 | 14 看了，你的意思是这篇文章就能推翻进化的事实了，不是这样的吧。只是有可能推翻过
去一条线的进化的方式，而有可能进化是多条线的而已。也有可能小的那块头盖骨化石，不是
人类的，所以可能还是一条线的进化。
Fossils in Kenya Challenge Linear Evolution
“Scientists who dated and analyzed the specimens — a 1.44 million-year-old
Homo habilis and a 1.55 million-year-old Homo erectus — said their
findings challenged the conventional view that these species evolved one
after the other. Instead, they apparently lived side by side in eastern
Africa for almost half a million years.”
这个是New York Times 的报道，意思是一样的。... 阅读全帖 |
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a***g
发帖数: 2402 | 15 当然是被业界认可的。
就比如说Haarlem specimen 其实是1855年被发现的，但当初分类错误。1970被重新确
认为始祖鸟化石，并被陈列于荷兰的泰勒斯博物馆。有时间有地点。你可以说我孤陋寡
闻不知道，但反戈一击指责别人没告诉你就有点儿不够谦卑了吧。 |
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w****i
发帖数: 634 | 16 奶奶的，看我的！
The paper is due on January 10, 2003. So you should start to write the
paper.
At the same time you need to conduct more experiments on specimens
with
attachments with smaller angles. I will be back to my office either
the night
of Dec 19 or Dec 20. I would like to discuss the progress with you on
Dec 20 or
21. Let me know if you can make it.
看到了么？偶老板给偶的信！ |
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m*********r
发帖数: 59 | 17 [瓜说，这个系列是他最不爱看的。结果是，朕犹豫了一下，今后是为他
方便，把他不爱看的内容都列到这个题目下，还是把他搅糊涂，把几个标
题乱re一气？这样的犹豫之下，是朕自己先糊涂，现在要说的，究竟是不
是瓜顶不爱看的内容。]
收到朋友寄来的两本书。这个朋友在网上写一些短篇小说，不太象通常
留学生一落笔就脱不了留学生活男男女女的巢臼，让朕耳目一新。他曾经
写过一个影评，当时朕去找DVD遍寻不得，弄得他很愧疚，许愿说写影评当
给朕寄碟，写书评当寄书。这次寄来的李洱的<<花腔>>，不知道算不算是
践约。为了不败人的兴趣，朕就不说<<花腔>>为啥好看，单说朕一口气看
完了就算。
另一本是<<一个人的村庄>>，作者刘亮程，朕孤陋，不认得。这本书的英
文名写的是"The village of a man", 这叫朕想起余华<<现实一种>>。余
华来卖书的时候朕才见到英译名是<>, 这个是“一
种现实”，朕向他表示口感远不如原名<<现实一种>>。若朕来选，必得叫
<> 来传达本名的力度。“一个人的村庄”给朕的
第一印象 |
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l*******s
发帖数: 3562 | 18 先声明一下，<> 是我的版权，Mousekiller剽窃了之后就诡称现
在记性不好对此学术腐败新动向悍然拒绝承认，我保留向方肘子检举的权利。
给作品取个好名字绝对是做作家或是学者的第一要务，万万轻忽不得的。这就是为什么孔
某人要把“狗日的北大”这样明显是拾人牙慧的名字也要先注册的原因。
取名字有很多种办法，自吹自擂是最低劣的，高手都不屑于使用。好比我每天Review
paper，如果标题就叫做“High Performance”云云的，通常作者都是Student Member。
而Princeton的Fellow就会做报告名为“从信号处理的角度看通信”(Communication:
from the perspective of signal processing)，何等气魄！虽然报告听下来也许还不如
Student Member那篇文章更有新意。查约翰霍普金斯大学物理系系主任当年的博士论文的
名字就叫做“关于宇宙学” about cosmology， 我们就可以知道这位先生为什么能够出
人头地。试问可还有第二人敢于如此给自己的论文起名乎？
中 |
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m*****a
发帖数: 999 | 19 灯塔水母
Turritopsis nutricula
http://en.wikipedia.org/wiki/Turritopsis_nutricula
It is the only known case of an animal capable of reverting completely to a
sexually immature, colonial stage after having reached sexual maturity as a
solitary stage. It does this through the cell development process of
transdifferentiation.
Theoretically, this process can go on indefinitely, effectively rendering
the jellyfish biologically immortal, although, in nature, most Turritopsis,
like other medusae, are likel... 阅读全帖 |
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r*****3
发帖数: 143 | 20 Have you ever wondered whether the forensic science you’ve seen on TV is
anything like the real thing? There’s no better way to find out than to
roll up your sleeves and do it yourself. This full-color book offers advice
for setting up an inexpensive home lab, and includes more than 50 hands-on
lab sessions that deal with forensic science experiments in biology,
chemistry, and physics. You’ll learn the practical skills and fundamental
knowledge needed to pursue forensics as a lifelong hobby—or e... 阅读全帖 |
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j***n
发帖数: 23 | 21 well summarized.
The main reason of super low S/N ratio in cryoEM is due to the dose-
sensitive of biological specimen to electron beam. The speed of electron in
modern EM is close to light-speed. After short exposure, almost every
chemical bond is gone, the structure is vaporized.
If the protein behaves like rock, EM may see atomic/sub-atomic
structure easily with current technology. |
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g**********0
发帖数: 180 | 22 frankly, many hospitals in China has complete clinical data...
and some hospital has set up bank of blood and tissue specimen.
The point is that we young doctors have to do research by ourselves...
otherwise you can only work for those so called leaders... |
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s******y
发帖数: 28562 | 23 It is more complicated than you said.
First, you need to remove the wax by xylene treatment,
then, you need to rehydrate the specimen by a serial concentration of
ethanol and finally the sodium citrate buffer
Then, boil in water
Finally, ready for staining.
I suggest you to obtain the complete protocol from a IHC handbook. |
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l**n
发帖数: 109 | 24 让我们回到最开始吧，我并没有说世上不存在V形的晶体，但当grasshopper在说
clustered crytals时你在说什么“break the twinned crystal is a usual method”
。一个对crystallography不了解的人把它们混为一谈也就算了，偏偏你又在这个
thread充大牛judge每一个人。有点看不惯而已。指出你的错误，免得误导别人。
"Twinning is a crystal growth disorder in which the specimen is composed of distinct domains whose orientations differ but are related in a particular, well-defined way. Sometimes non-convex crystal morphology implicates twinning, but microscopic examination is often uninformative. Twinning usually pre |
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w********e
发帖数: 110 | 25 A postdoctoral position is available immediately in the Signal Transduction
Laboratory, Department of Gynecology and Obstetrics at St. Joseph’s
Hospital and Medical Center located in Phoenix, Arizona. Our laboratory is
focused on the study of signal transduction pathways and implications in
endocrine-related cancers, diabetes, and obesity, using cell culture system,
animal models as well as specimens from human subjects. Our long-term goal
is to discover potential therapeutic biomarkers and ne |
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l*h
发帖数: 4124 | 26 typically, if the employee works at least 17.5hrs (20hrs for other employers
) a week and has direct patient or human specimen/blood contact, the
employer buys the employee health insurance.
but for retirement benefits, hourly-paid employees typically have long
vesting period.
benefit
it is not a full time position in a hospital, the benefit will be not good
or no |
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K**********e
发帖数: 188 | 27 Gene
Volume 427, Issues 1-2, 31 December 2008, Pages 7-18
Evolution of Pacific Ocean and the Sea of Japan populations of the gobiid
species, Pterogobius elapoides and
Pterogobius zonoleucus, based on molecular and morphological analyses
Akihitoa, Akishinonomiya Fumihitob, c, Yuji Ikedad, Masahiro Aizawad,
Takashi Makinoe, 1, Yumi
Umeharae, Yoshiaki Kaif, Yuriko Nishimotob, g, Masami Hasegawab, e, h,
Tetsuji Nakaboi and Takashi
Gojoborib, e,
aThe Imperial Residence, 1-1 Chiyoda, Chiyoda-ku,... 阅读全帖 |
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p*******r
发帖数: 59 | 28 Career Opportunities at JCVI Infectious Disease (8 Positions)
The PIs did not ask me to post here, I did this just in case someone is
looking for similar positions but did not see these, and I would be happy to
see some chinese fellows to come. All the PIs are very nice.
All listed at
https://careers.jcvi.org/careers/Careers.aspx
http://www.jcvi.org/
No. 1 & No. 2 Post-Doctoral Fellows
Job Responsibilities
JCVI is looking for two Post-Doctoral Fellows to join the Infectious Disease
Group in our ... 阅读全帖 |
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w********e
发帖数: 110 | 29 A postdoctoral fellow is needed immediately in the Signal Transduction
Laboratory, Department of Gynecology and Obstetrics, in collaboration with
Dept of Neurology, Barrow Neurological Institute at St. Joseph’s Hospital
and Medical Center located in Phoenix, Arizona. The research in our
laboratories is focused on signal transduction pathways in cancers, diabetes
, and hypothalamic hamartoma (HH). Cell culture systems, animal models, as
well as specimens from human subjects are routinely used in... 阅读全帖 |
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t*d
发帖数: 1290 | 30 http://www.genomeweb.com//node/980559?hq_e=el&hq_m=1103092&hq_l
----------------------------------------
A recent study by scientists at Yale University suggests that the actual
cost of sequencing may be much higher than some current estimates indicate
since those figures may not factor in the analysis costs that are necessary
for a successful sequencing project.
In the paper, published in Genome Biology last month, Yale's Mark Gerstein
and colleagues consider costs that weren’t taken into accou... 阅读全帖 |
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l**********1
发帖数: 5204 | 31 庄只是徐福炼丹 给秦始皇炼那个长生不老丹吧?
Xiaoliang Sunney Xie
Raman Scattering is King Route to 单分子Raman scattering 光 (possible)
1)
Single DNA molecule detection in an optical trap using surface-enhanced Raman scattering
//apl.aip.org/resource/1/applab/v96/i21/p213701_s1
Abstract:
Raman spectra from single DNA molecules in their natural aqueous environment are presented. A DNA molecule that is anchored between two optically trapped dielectric beads is suspended in a solution with nanosized silver colloid particl... 阅读全帖 |
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l**********1
发帖数: 5204 | 32 Cryo-Electron Tomography
Cryo-ET is a very accurate way to determine the three-dimensional structure
of a specimen because the rapid freezing of the object and cryogenic
temperatures gives a good preservation of the structure and good time
resolution of certain processes. For example, the rapid freezing of cells
and tissues at a certain point in cellular processes can give a good
understanding of the structure and activity of those cells and tissues at a
certain point in time during that particu... 阅读全帖 |
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a*******y
发帖数: 221 | 33 是一个针对transparent一个针对opaque的specimen么
谢谢 |
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o******h
发帖数: 198 | 35 Technical Lab Manager (Clinical Trials) - in Shanghai, CHINA. Relocation
assistance available.
See job description below:
supervise the laboratory team (2 x scientists, 6 x medical technologists, 1
x Specimen Processor/SMAS Coordinator).
Good verbal and written English & Mandarin
China salary range:
Chinese national: RMB336,000 – RMB373,500 per annum, with benefits
Non-Chinese national: RMB378,000.00 – RMB415,500.00, no benefits
The salary interpretation is as follows for US candidates:
Chinese ... 阅读全帖 |
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z******7
发帖数: 1 | 36 Job Title:
Research/Laboratory Technician II, Biomedical Engineering, Washington
University in St. Louis - 25623
Full-Time
Essential Functions
This position assists with the technical and organizational aspects of
studies and experiments of a biomedical engineering laboratory.
- Perform experiments, including collecting and analyzing experimental data
(55%)
- Enter data, and maintaining data files from research experiments (20%)
- Attend lab meetings, ordering lab supplies, and handling of hazar... 阅读全帖 |
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e*******o
发帖数: 4654 | 37 Job Title:
Research/Laboratory Technician II, Biomedical Engineering, Washington
University in St. Louis - 25623
Full-Time
Essential Functions
This position assists with the technical and organizational aspects of
studies and experiments of a biomedical engineering laboratory.
- Perform experiments, including collecting and analyzing experimental data
(55%)
- Enter data, and maintaining data files from research experiments (20%)
- Attend lab meetings, ordering lab supplies, and handling of hazar... 阅读全帖 |
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l********n
发帖数: 260 | 38 揭露臧敬五在新闻媒体上的谎言——兼评其JCI论文
作者：胆大妄为
方舟子先生，
你好！本人注意到臧敬五在很多新闻媒体上对其作为通讯作者发表在JCI上
的论文加以宣传后，翻阅了这篇论文（1），发现臧敬五胆大妄为，编造谎言，
欺骗中国新闻媒体，应予以揭露。
先看二篇臧敬五在媒体上的宣传：
“骨桥蛋白”是引发类风湿性关节炎的顽凶
新华网上海３月２日电（胡德荣、仇逸）严重影响人类健康和生活质量的类
风湿性关节炎（注：rheumatoid arthritis，RA）研究获得重大突破性进展。我
国科研人员在国际上第一次阐明了骨桥蛋白（注：osteopontin ，OPN）在类风
湿性关节炎发病中的作用，发现“骨桥蛋白”是引发类风湿性关节炎的顽凶，为
治疗类风湿性关节炎找到了临床手段和药物靶点。……
http://www.stcsm.gov.cn/news/detail.asp?pid=64857
我专家突破类风湿关节炎治疗世界难题
上海分院
日前，中国科学院上海生命科学研究院健康科学中心主任臧敬五教授，带领
科研人员完成了“骨桥蛋白在类风湿性关节炎中的病理机制”研究，其论文已刊
登在3月2日出版的... 阅读全帖 |
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m******g
发帖数: 467 | 39 Nature 14.07.03
稍微了解一下：
1. Analysis of orthologous groups reveals archease and DDX1 as tRNA splicing
factors
小知识：
1. Evolutionary developmental biology: Use it or lose it
Multiple avenues to digit loss
Patterning and post-patterning modes of evolutionary digit loss in mammals
2. New specimen of Archaeopteryx provides insights into the evolution of
pennaceous feathers
Most likely display -> exaptation, later recruited for aerodynamic functions |
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c*z
发帖数: 157 | 40 手术或者biopsy出来的东西一般两种处理，
一个是最常见的泡福尔马林然后装石蜡里面，用来切片，一般这个不会frozen，4度冰
箱保存
另一个是最近才开始流行的泡oct里面snap frozen，现在越来越多有这个option, 但不
是必须做的
你说的两个biopsy是1mm直径的吧？这种一般都是横过来放石蜡里面做切片的，头几层
太小基本不能用，切到中间界面比较大了才会拿来染色，所以第一个病理报告出来的时
候一半tissue基本已经没了
医院答复不会给外面是正常的，医院转运specimens都要material transfer agreement
, 很麻烦，没人高兴给你做的
关键是，当时到底有没有存frozen的？如果没有存frozen的话，你投诉也没用；或者说
，如果你没办法举证它们有frozen, 这个就没办法了，试试别的测试吧，让收孩子的下
家想想办法
诊。 |
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b****r
发帖数: 17995 | 41 他第一个培养了这种病原体，不是找到了”新的培养方法“
11年后才有人找到 第 二 种培养沙眼衣原体的办法
50 years later the trachoma agent was cultured by
T'ang, Huang, Chang, and Wong (1957) in the yolk
sacs of fertile hens' eggs.
The technique of culture in irradiated McCoy
cells (Gordon, Harper, Quan, Treharne, Dwyer,
and Garland, 1969) provided the first alternative to
culture in yolk sac for the primary isolation of
Chlamydia.
详见
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1048360/pdf/brjvendis00078-0008
.pdf
The inclusion bodies of TRIC (t... 阅读全帖 |
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b*****b
发帖数: 14 | 42 Cutan Ocul Toxicol. 2013 Mar;32(1):46-53. doi: 10.3109/15569527.2012.705405.
Epub 2012 Aug 6.
Limitations of an ocular surface inflammatory biomarker in impression
cytology specimens.
Yafawi R1, Ko M, Sace FP, John-Baptiste A. |
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t**********t
发帖数: 141 | 43 that's not entirely true.
medical genetics MOSTLY does not interpret pathological tissue, they simply
don't
have access to the pathological tissue specimen.
tumor (both solid and blood-borne) genetics are NORMALLY interpreted by the
molecular
pathologist within the pathology department.
guidelines |
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t**********t
发帖数: 141 | 44 LOL! Of course anatomic pathologists do not follow ACMG guideline, why
should them? On the other hand, the medical geneticists do not have the
capacity to interpret tumor specimen either, they are not trained to do so.
tumor |
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T*G
发帖数: 600 | 45 我只是简单说一下为什么NGS在Molecular Pathology 现阶段应用很有限，看来你没接
触过NGS interpretation, NGS reliable的interpretation和database大多应用在
prenatal 和一些遗传疾病的检测，为了不同的reference lab 间对case 有相同的
interpretation, ACMG在业内定了disease interpretation 的guideline。"the
medical geneticists do not have the capacity to interpret tumor specimen
either" This is totally wrong, ARE U NUTS? look at how many labs are doing
tumor NGS in both academia and industry. The major difficulty is not
freaking how you get tumor samples, it is how you inte... 阅读全帖 |
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S*********e
发帖数: 127 | 46 Thanks.
1.
Recommendations for the evaluation of specimen stability for flow cytometric
testing during drug development.
Brown L, Green CL, Jones N, Stewart JJ, Fraser S, Howell K, Xu Y, Hill CG,
Wiwi CA, White WI, O'Brien PJ, Litwin V.
J Immunol Methods. 2015 Mar;418:1-8. doi: 10.1016/j.jim.2015.01.008. Epub
2015 Feb 4. Review.
PMID: 25662815
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